Prostacyclin receptor: Difference between revisions

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imported>Ser Amantio di Nicolao
 
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{{Infobox_gene}}
{{Infobox_gene}}
The '''Prostacyclin receptor ''', also termed the '''prostaglandin I2 receptor''' or just '''IP''', is a [[receptor (biochemistry)|receptor]] belonging to the [[prostaglandin receptor|prostaglandin]] (PG) group of receptors. IP binds to and mediates the biological actions of [[prostacyclin]] (also termed Prostaglandin I<sub>2</sub>, PGI<sub>2</sub>, or when used as a drug, epoprostenol). IP is encoded in humans by the ''PTGIR'' [[gene]]. While possessing many functions as defined in animal model studies, the major clinical relevancy of IP is as a powerful vasodilator: stimulators of IP are used to treat severe and even life-threatening diseases involving pathological [[vasoconstriction]].
The '''Prostacyclin receptor ''', also termed the '''prostaglandin I2 receptor''' or just '''IP''', is a [[receptor (biochemistry)|receptor]] belonging to the [[prostaglandin receptor|prostaglandin (PG) group of receptors]]. IP binds to and mediates the biological actions of [[prostacyclin]] (also termed Prostaglandin I<sub>2</sub>, PGI<sub>2</sub>, or when used as a drug, epoprostenol). IP is encoded in humans by the ''PTGIR'' [[gene]]. While possessing many functions as defined in animal model studies, the major clinical relevancy of IP is as a powerful vasodilator: stimulators of IP are used to treat severe and even life-threatening diseases involving pathological [[vasoconstriction]].
 
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== Gene ==
== Gene ==
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== Expression ==
== Expression ==
IP is most highly expressed in brain and [[thymus]] and is readily detected in most other tissues. It is found throughout the vascular network on [[endothelium]] and [[smooth muscle]] cells.<ref name="ncbi.nlm.nih.gov"/><ref name="pmid25541289">{{cite journal | vauthors = Claar D, Hartert TV, Peebles RS | title = The role of prostaglandins in allergic lung inflammation and asthma | journal = Expert Review of Respiratory Medicine | volume = 9 | issue = 1 | pages = 55–72 | year = 2015 | pmid = 25541289 | pmc = 4380345 | doi = 10.1586/17476348.2015.992783 | url = }}</ref>
IP is most highly expressed in brain and [[thymus]] and is readily detected in most other tissues. It is found throughout the vascular network on [[endothelium]] and [[smooth muscle]] cells.<ref name="ncbi.nlm.nih.gov"/><ref name="pmid25541289">{{cite journal | vauthors = Claar D, Hartert TV, Peebles RS | title = The role of prostaglandins in allergic lung inflammation and asthma | journal = Expert Review of Respiratory Medicine | volume = 9 | issue = 1 | pages = 55–72 | date = February 2015 | pmid = 25541289 | pmc = 4380345 | doi = 10.1586/17476348.2015.992783 }}</ref>


== Ligands ==
== Ligands ==
=== Activating ligands ===
=== Activating ligands ===
Standard [[prostanoid]]s have the following relative efficacies as [[Ligand (biochemistry)|receptor ligands]] in binding to and activating IP: PGI<sub>2</sub>>>[[PGD2]]=[[PGE2]]=[[PGF2 alpha|PGF2α]]>[[TXA2]]. In typical binding studies, PGI<sub>2</sub> has one-half of its maximal binding capacity and cell-stimulating actions at ~1 [[Molar concentration#Units|nanomolar]] whereas the other prostaglandins are >50-fold to 100-fold weaker than this. However, PGI<sub>2</sub> is very unstable, spontaneously converting to a far less active derivative [[Prostacyclin#degradation|6-keto-PGF1 alpha]] within 1 minute of its formation. This instability makes defining the exact affinity of PGI<sub>2</sub> for IP difficult. It also makes it important to have stable synthetic analogs of PGI<sub>2</sub> for clinical usage. The most potent of these [[receptor agonists]] for binding to and activating IP are [[iloprost]], taprostene, and esuberaprost which have [[Dissociation Constant|K<sub>d</sub>]] values (i.e. concentrations which bind to half of available IP receptors) in the low nana[[Mole (unit)|mole]]/liter range (http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=345/).<ref name="pmid21508345">{{cite journal | vauthors = Ricciotti E, FitzGerald GA | title = Prostaglandins and inflammation | journal = Arteriosclerosis, Thrombosis, and Vascular Biology | volume = 31 | issue = 5 | pages = 986–1000 | year = 2011 | pmid = 21508345 | pmc = 3081099 | doi = 10.1161/ATVBAHA.110.207449 | url = }}</ref>
Standard [[prostanoid]]s have the following relative efficacies as [[Ligand (biochemistry)|receptor ligands]] in binding to and activating IP: PGI<sub>2</sub>>>[[PGD2]]=[[PGE2]]=[[PGF2 alpha|PGF2α]]>[[TXA2]]. In typical binding studies, PGI<sub>2</sub> has one-half of its maximal binding capacity and cell-stimulating actions at ~1 [[Molar concentration#Units|nanomolar]] whereas the other prostaglandins are >50-fold to 100-fold weaker than this. However, PGI<sub>2</sub> is very unstable, spontaneously converting to a far less active derivative [[Prostacyclin#degradation|6-keto-PGF1 alpha]] within 1 minute of its formation. This instability makes defining the exact affinity of PGI<sub>2</sub> for IP difficult. It also makes it important to have stable synthetic analogs of PGI<sub>2</sub> for clinical usage. The most potent of these [[receptor agonists]] for binding to and activating IP are [[iloprost]], taprostene, and esuberaprost which have [[Dissociation Constant|K<sub>d</sub>]] values (i.e. concentrations which bind to half of available IP receptors) in the low nana[[Mole (unit)|mole]]/liter range (http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=345/).<ref name="pmid21508345">{{cite journal | vauthors = Ricciotti E, FitzGerald GA | title = Prostaglandins and inflammation | journal = Arteriosclerosis, Thrombosis, and Vascular Biology | volume = 31 | issue = 5 | pages = 986–1000 | date = May 2011 | pmid = 21508345 | pmc = 3081099 | doi = 10.1161/ATVBAHA.110.207449 }}</ref>


=== Inhibiting ligands ===
=== Inhibiting ligands ===
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== Mechanism of cell activation ==
== Mechanism of cell activation ==
IP is classified as a relaxant type of prostenoid receptor based on its ability, upon activation, to relax certain pre-contracted smooth muscle preparations and smooth muscle-containing tissues such as those of pulmonary arteries and veins.<ref name="pmid18709530">{{cite journal | vauthors = Matsuoka T, Narumiya S | title = The roles of prostanoids in infection and sickness behaviors | journal = Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy | volume = 14 | issue = 4 | pages = 270–8 | year = 2008 | pmid = 18709530 | doi = 10.1007/s10156-008-0622-3 | url = }}</ref> When bound to PGI<sub>2</sub> or other of its agonists, IP stimulates one or more of three types of [[G protein]] complexes, depending on cell type: '''a)''' [[Gs alpha subunit]]-[[G beta-gamma complex|Gβγ]] complexes which release Gs that then stimulates [[adenyl cyclase]] to raise intracellular levels of [[Cyclic adenosine monophosphate#Role in eukaryotic cells|cAMP]] and thereby activate cAMP-regulated protein kinases A-dependent [[cell signal]]ing pathways (see [[protein kinase A#Function|PKA]]); '''b)''' [[Gq alpha subunit]]-Gβγ complexes which release Gq that then stimulates other cell signaling pathways (e.g. [[phospholipase C]]/[[Inositol trisphosphate|IP3]]/cell [[Calcium in biology|Ca<sup>2+</sup>]] mobilization/[[diacylglycerol]]/[[protein kinase C]]s, [[calmodulin]]-modulated [[myosin light chain kinase]], [[RAF]]/[[Mitogen-activated protein kinase kinase|MEK]]/[[Mitogen-activated protein kinase]]s, PKC/Ca<sup>2+</sup>/[[Calcineurin]]/[[Nuclear factor of activated T-cells]]; and [[Epidermal growth factor|EGF]] cellular receptors; and '''c)''' [[Gi alpha subunit]]-Giβγ) complexes which releases Gi that then simulates [[phospholipase C]] to cleave phosphatidylinositol triphosphate into [[inositol triphosphate]] that raises intracellular CaCa<sup>2</sup> levels thereby regulating [[Calcium signaling]] pathways and [[diacylglycerol]] that activates certain [[protein kinase C]] enzymes )that phosphorylate and thereby regulate target proteins involved in cell signaling (see [[Protein kinase C#Function]]). Studies suggest that stimulation of Gsβγ complexes is required for activation of the Gqβγ- and Giβγ-dependent pathways.<ref name="pmid21508345"/><ref name="pmid18946232">{{cite journal | vauthors = Oguma T, Asano K, Ishizaka A | title = Role of prostaglandin D(2) and its receptors in the pathophysiology of asthma | journal = Allergology International | volume = 57 | issue = 4 | pages = 307–12 | year = 2008 | pmid = 18946232 | doi = 10.2332/allergolint.08-RAI-0033 | url = }}</ref><ref name="pmid21752876">{{cite journal | vauthors = Woodward DF, Jones RL, Narumiya S | title = International Union of Basic and Clinical Pharmacology. LXXXIII: classification of prostanoid receptors, updating 15 years of progress | journal = Pharmacological Reviews | volume = 63 | issue = 3 | pages = 471–538 | year = 2011 | pmid = 21752876 | doi = 10.1124/pr.110.003517 | url = }}</ref><ref name="pmid27940058">{{cite journal | vauthors = Moreno JJ | title = Eicosanoid receptors: Targets for the treatment of disrupted intestinal epithelial homeostasis | journal = European Journal of Pharmacology | volume = 796 | issue = | pages = 7–19 | year = 2017 | pmid = 27940058 | doi = 10.1016/j.ejphar.2016.12.004 | url = }}</ref> In certain cells, activation of FP also stimulates [[G12/G13 alpha subunits|G<sup>12</sup>/G<sup>13</sup>]]-Gβγ G proteins to activate the [[Rho family of GTPases]] signaling proteins and [[Gi alpha subunit|Gi]]-Gβγ G proteins to activate[[Raf kinase|Raf]]/MEK/mitogen-activated kinase pathways.
IP is classified as a relaxant type of prostenoid receptor based on its ability, upon activation, to relax certain pre-contracted smooth muscle preparations and smooth muscle-containing tissues such as those of pulmonary arteries and veins.<ref name="pmid18709530">{{cite journal | vauthors = Matsuoka T, Narumiya S | title = The roles of prostanoids in infection and sickness behaviors | journal = Journal of Infection and Chemotherapy | volume = 14 | issue = 4 | pages = 270–8 | date = August 2008 | pmid = 18709530 | doi = 10.1007/s10156-008-0622-3 }}</ref> When bound to PGI<sub>2</sub> or other of its agonists, IP stimulates one or more of three types of [[G protein]] complexes, depending on cell type: '''a)''' [[Gs alpha subunit]]-[[G beta-gamma complex|Gβγ]] complexes which release Gs that then stimulates [[adenyl cyclase]] to raise intracellular levels of [[Cyclic adenosine monophosphate#Role in eukaryotic cells|cAMP]] and thereby activate cAMP-regulated protein kinases A-dependent [[cell signal]]ing pathways (see [[protein kinase A#Function|PKA]]); '''b)''' [[Gq alpha subunit]]-Gβγ complexes which release Gq that then stimulates other cell signaling pathways (e.g. [[phospholipase C]]/[[Inositol trisphosphate|IP3]]/cell [[Calcium in biology|Ca<sup>2+</sup>]] mobilization/[[diacylglycerol]]/[[protein kinase C]]s, [[calmodulin]]-modulated [[myosin light chain kinase]], [[RAF]]/[[Mitogen-activated protein kinase kinase|MEK]]/[[Mitogen-activated protein kinase]]s, PKC/Ca<sup>2+</sup>/[[Calcineurin]]/[[Nuclear factor of activated T-cells]]; and [[Epidermal growth factor|EGF]] cellular receptors; and '''c)''' [[Gi alpha subunit]]-Giβγ) complexes which releases Gi that then simulates [[phospholipase C]] to cleave phosphatidylinositol triphosphate into [[inositol triphosphate]] that raises intracellular CaCa<sup>2</sup> levels thereby regulating [[Calcium signaling]] pathways and [[diacylglycerol]] that activates certain [[protein kinase C]] enzymes )that phosphorylate and thereby regulate target proteins involved in cell signaling (see [[Protein kinase C#Function]]). Studies suggest that stimulation of Gsβγ complexes is required for activation of the Gqβγ- and Giβγ-dependent pathways.<ref name="pmid21508345"/><ref name="pmid18946232">{{cite journal | vauthors = Oguma T, Asano K, Ishizaka A | title = Role of prostaglandin D(2) and its receptors in the pathophysiology of asthma | journal = Allergology International | volume = 57 | issue = 4 | pages = 307–12 | date = December 2008 | pmid = 18946232 | doi = 10.2332/allergolint.08-RAI-0033 }}</ref><ref name="pmid21752876">{{cite journal | vauthors = Woodward DF, Jones RL, Narumiya S | title = International Union of Basic and Clinical Pharmacology. LXXXIII: classification of prostanoid receptors, updating 15 years of progress | journal = Pharmacological Reviews | volume = 63 | issue = 3 | pages = 471–538 | date = September 2011 | pmid = 21752876 | doi = 10.1124/pr.110.003517 }}</ref><ref name="pmid27940058">{{cite journal | vauthors = Moreno JJ | title = Eicosanoid receptors: Targets for the treatment of disrupted intestinal epithelial homeostasis | journal = European Journal of Pharmacology | volume = 796 | issue = | pages = 7–19 | date = February 2017 | pmid = 27940058 | doi = 10.1016/j.ejphar.2016.12.004 }}</ref> In certain cells, activation of FP also stimulates [[G12/G13 alpha subunits|G<sup>12</sup>/G<sup>13</sup>]]-Gβγ G proteins to activate the [[Rho family of GTPases]] signaling proteins and [[Gi alpha subunit|Gi]]-Gβγ G proteins to activate[[Raf kinase|Raf]]/MEK/mitogen-activated kinase pathways.


== Functions ==
== Functions ==
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=== Platelets ===
=== Platelets ===
IP [[gene knockout]] mice (i.e. IP(-/-) mice) exhibit increased tendency to [[thrombosis]] in response to experimentally-induced [[Endothelium]], a result which appears to reflect, at least in part, the loss of IP's anti-platelet activity.<ref name="pmid26980701">{{cite journal | vauthors = Stitham J, Hwa J | title = Prostacyclin, Atherothrombosis and Diabetes Mellitus: Physiologic and Clinical Considerations | journal = Current Molecular Medicine | volume = 16 | issue = 4 | pages = 328–42 | year = 2016 | pmid = 26980701 | doi = | url = }}</ref><ref name="pmid10508233">{{cite journal | vauthors = Narumiya S, Sugimoto Y, Ushikubi F | title = Prostanoid receptors: structures, properties, and functions | journal = Physiological Reviews | volume = 79 | issue = 4 | pages = 1193–226 | year = 1999 | pmid = 10508233 | doi = | url = }}</ref> IP activation of animal and human [[platelets]] inhibits their [[Platelet#aggregation|aggreatation]] response and as one consequence of this inhibition of platelet-dependent [[blood clotting]]. The PGI<sub>2</sub>-IP axis along with the production of [[Nitric Oxide#Functions|nitric oxide]], acting together additively and potentially synergistically, are powerful and physiological negative regulators of platelet function and thereby blood clotting in humans. Studies suggest that the <sub>2</sub>-IP axis is impaired in patients with a tendency to develop pathological [[thrombosis]] such as occurs in obesity, diabetes, and [[coronary artery disease]].<ref name="pmid21752876"/><ref name="pmid27358171">{{cite journal | vauthors = Procter NE, Hurst NL, Nooney VB, Imam H, De Caterina R, Chirkov YY, Horowitz JD | title = New Developments in Platelet Cyclic Nucleotide Signalling: Therapeutic Implications | journal = Cardiovascular Drugs and Therapy | volume = 30 | issue = 5 | pages = 505–513 | year = 2016 | pmid = 27358171 | doi = 10.1007/s10557-016-6671-4 | url = }}</ref>
IP [[gene knockout]] mice (i.e. IP(-/-) mice) exhibit increased tendency to [[thrombosis]] in response to experimentally-induced [[Endothelium]], a result which appears to reflect, at least in part, the loss of IP's anti-platelet activity.<ref name="pmid26980701">{{cite journal | vauthors = Stitham J, Hwa J | title = Prostacyclin, Atherothrombosis and Diabetes Mellitus: Physiologic and Clinical Considerations | journal = Current Molecular Medicine | volume = 16 | issue = 4 | pages = 328–42 | year = 2016 | pmid = 26980701 | doi = 10.2174/1566524016666160316150728}}</ref><ref name="pmid10508233">{{cite journal | vauthors = Narumiya S, Sugimoto Y, Ushikubi F | title = Prostanoid receptors: structures, properties, and functions | journal = Physiological Reviews | volume = 79 | issue = 4 | pages = 1193–226 | date = October 1999 | pmid = 10508233 | doi = 10.1152/physrev.1999.79.4.1193 }}</ref> IP activation of animal and human [[platelets]] inhibits their [[Platelet#aggregation|aggreatation]] response and as one consequence of this inhibition of platelet-dependent [[blood clotting]]. The PGI<sub>2</sub>-IP axis along with the production of [[Nitric Oxide#Functions|nitric oxide]], acting together additively and potentially synergistically, are powerful and physiological negative regulators of platelet function and thereby blood clotting in humans. Studies suggest that the <sub>2</sub>-IP axis is impaired in patients with a tendency to develop pathological [[thrombosis]] such as occurs in obesity, diabetes, and [[coronary artery disease]].<ref name="pmid21752876"/><ref name="pmid27358171">{{cite journal | vauthors = Procter NE, Hurst NL, Nooney VB, Imam H, De Caterina R, Chirkov YY, Horowitz JD | title = New Developments in Platelet Cyclic Nucleotide Signalling: Therapeutic Implications | journal = Cardiovascular Drugs and Therapy | volume = 30 | issue = 5 | pages = 505–513 | date = October 2016 | pmid = 27358171 | doi = 10.1007/s10557-016-6671-4 }}</ref>


=== Cardiovascular system ===
=== Cardiovascular system ===
IP activation stimulates the dilation of arteries and veins in various animal models as well as in humans. It increases the blood flow through, for example, the pulmonary, coronary, retinal and [[choroid]] circulation. Inhaled PGI<sub>2</sub> causes a modest fall in [[diastolic]] and small fall in systolic blood pressure in humans. This action involves IP's ability to relax vascular smooth muscle and is considered to be one of the fundamental functions of IP receptors. Furthermore, IP(-/-) mice on a high salt diet develop significantly higher levels of [[hypertension]], cardiac fibrosis, and cardiac [[Cardiomegaly|hypertrophy]] than control mice. The vasodilating and, perhaps, platelet-inhibiting effects of IP receptors likely underlie its ability suppress hypertension and protect tissues such as the heart in this model as well as the heart, brain, and gastrointestinal tract in various animal models of [[Ischemia|ischemic]] injury.<ref name="pmid21752876"/> Indeed, IP agonists are used to treat patients pathological [[vasoconstriction]] diseases.<ref name="pmid23850788">{{cite journal | vauthors = Benyahia C, Boukais K, Gomez I, Silverstein A, Clapp L, Fabre A, Danel C, Leséche G, Longrois D, Norel X | title = A comparative study of PGI2 mimetics used clinically on the vasorelaxation of human pulmonary arteries and veins, role of the DP-receptor | journal = Prostaglandins & Other Lipid Mediators | volume = 107 | issue = | pages = 48–55 | year = 2013 | pmid = 23850788 | doi = 10.1016/j.prostaglandins.2013.07.001 | url = }}</ref> The injection of IP activators into the skin of rodents increases local capillary permeability and swelling; IP(-/-) mice fail to show this increased capillary permeability and swelling in response not only to IP activators but also in a model of carrageenan- or [[bradykinin]]-induced paw edema. IP antagonists likewise reduce experimentally-induced capillary permeability and swelling in rats. This actions is also considered a physiological function of IP receptors,<ref name="pmid21508345"/><ref name="pmid21752876"/> but can contribute to the toxicity of IP activators in patients by inducing, for example, life-threatening [[pulmonary edema]].<ref name="pmid23850788"/>
IP activation stimulates the dilation of arteries and veins in various animal models as well as in humans. It increases the blood flow through, for example, the pulmonary, coronary, retinal and [[choroid]] circulation. Inhaled PGI<sub>2</sub> causes a modest fall in [[diastolic]] and small fall in systolic blood pressure in humans. This action involves IP's ability to relax vascular smooth muscle and is considered to be one of the fundamental functions of IP receptors. Furthermore, IP(-/-) mice on a high salt diet develop significantly higher levels of [[hypertension]], cardiac fibrosis, and cardiac [[Cardiomegaly|hypertrophy]] than control mice. The vasodilating and, perhaps, platelet-inhibiting effects of IP receptors likely underlie its ability suppress hypertension and protect tissues such as the heart in this model as well as the heart, brain, and gastrointestinal tract in various animal models of [[Ischemia|ischemic]] injury.<ref name="pmid21752876"/> Indeed, IP agonists are used to treat patients pathological [[vasoconstriction]] diseases.<ref name="pmid23850788">{{cite journal | vauthors = Benyahia C, Boukais K, Gomez I, Silverstein A, Clapp L, Fabre A, Danel C, Leséche G, Longrois D, Norel X | title = A comparative study of PGI2 mimetics used clinically on the vasorelaxation of human pulmonary arteries and veins, role of the DP-receptor | journal = Prostaglandins & Other Lipid Mediators | volume = 107 | issue = | pages = 48–55 | date = December 2013 | pmid = 23850788 | doi = 10.1016/j.prostaglandins.2013.07.001 }}</ref> The injection of IP activators into the skin of rodents increases local capillary permeability and swelling; IP(-/-) mice fail to show this increased capillary permeability and swelling in response not only to IP activators but also in a model of carrageenan- or [[bradykinin]]-induced paw edema. IP antagonists likewise reduce experimentally-induced capillary permeability and swelling in rats. This actions is also considered a physiological function of IP receptors,<ref name="pmid21508345"/><ref name="pmid21752876"/> but can contribute to the toxicity of IP activators in patients by inducing, for example, life-threatening [[pulmonary edema]].<ref name="pmid23850788"/>


IP activators inhibit the adherence of circulating platelets and leukocytes adherence to vascular endothelium thereby blocking their entry into sites of tissue disturbance. The activators also inhibit vascular smooth muscle cells from proliferation by blocking these cells' [[cell cycle|growth cycle]] and triggering their [[apoptosis]] (i.e. cell death]]. These actions, along with its anti-inflammatory effects, may underlie the ability of IP gene knockout in an ApoE(−/−) mosue model to cause an accelerated rate of developing atherosclerosis.<ref name="pmid21508345"/>
IP activators inhibit the adherence of circulating platelets and leukocytes adherence to vascular endothelium thereby blocking their entry into sites of tissue disturbance. The activators also inhibit vascular smooth muscle cells from proliferation by blocking these cells' [[cell cycle|growth cycle]] and triggering their [[apoptosis]] (i.e. cell death]]. These actions, along with its anti-inflammatory effects, may underlie the ability of IP gene knockout in an ApoE(−/−) mosue model to cause an accelerated rate of developing atherosclerosis.<ref name="pmid21508345"/>
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== Clinical significance ==
== Clinical significance ==
=== Toxicity ===
=== Toxicity ===
IP receptor agonists, particularly when used intravenously, have been associated with the rapid development of pulmonary edema, hypotension, bleeding due to inhibition of platelet aggregation, and tachycardia.<ref name="pmid26438636">{{cite journal | vauthors = McGinn K, Reichert M | title = A Comparison of Inhaled Nitric Oxide Versus Inhaled Epoprostenol for Acute Pulmonary Hypertension Following Cardiac Surgery | journal = The Annals of Pharmacotherapy | volume = 50 | issue = 1 | pages = 22–6 | year = 2016 | pmid = 26438636 | doi = 10.1177/1060028015608865 | url = }}</ref><ref name="pmid26294418">{{cite journal | vauthors = Searcy RJ, Morales JR, Ferreira JA, Johnson DW | title = The role of inhaled prostacyclin in treating acute respiratory distress syndrome | journal = Therapeutic Advances in Respiratory Disease | volume = 9 | issue = 6 | pages = 302–12 | year = 2015 | pmid = 26294418 | doi = 10.1177/1753465815599345 | url = }}</ref> Clinical use of these agonists is contraindicated in patients suffering many conditions. For example, the IP agonist [[iloprost#Important safety information|iloprost]] is contraindicated in patients with unstable [[angina]]; decompensated [[cardiac failure]] (unless under close medical supervision); severe [[cardiac arrhythmias]]; congenital or acquired [[heart valve]] defects; increased risk of bleeding; a history of [[myocardial infarction]] in the past 6 months; or a history of cerebrovascular events (e.g. stroke) within 3 months.
IP receptor agonists, particularly when used intravenously, have been associated with the rapid development of pulmonary edema, hypotension, bleeding due to inhibition of platelet aggregation, and tachycardia.<ref name="pmid26438636">{{cite journal | vauthors = McGinn K, Reichert M | title = A Comparison of Inhaled Nitric Oxide Versus Inhaled Epoprostenol for Acute Pulmonary Hypertension Following Cardiac Surgery | journal = The Annals of Pharmacotherapy | volume = 50 | issue = 1 | pages = 22–6 | date = January 2016 | pmid = 26438636 | doi = 10.1177/1060028015608865 }}</ref><ref name="pmid26294418">{{cite journal | vauthors = Searcy RJ, Morales JR, Ferreira JA, Johnson DW | title = The role of inhaled prostacyclin in treating acute respiratory distress syndrome | journal = Therapeutic Advances in Respiratory Disease | volume = 9 | issue = 6 | pages = 302–12 | date = December 2015 | pmid = 26294418 | doi = 10.1177/1753465815599345 }}</ref> Clinical use of these agonists is contraindicated in patients suffering many conditions. For example, the IP agonist [[iloprost#Important safety information|iloprost]] is contraindicated in patients with unstable [[angina]]; decompensated [[cardiac failure]] (unless under close medical supervision); severe [[cardiac arrhythmias]]; congenital or acquired [[heart valve]] defects; increased risk of bleeding; a history of [[myocardial infarction]] in the past 6 months; or a history of cerebrovascular events (e.g. stroke) within 3 months.


=== Vasoconstriction ===
=== Vasoconstriction ===
IP receptor agonists are front-line drugs to treat [[pulmonary hypertension]]. Major drugs in this category include PGI<sub>2</sub> itself (i.e. [[epoprostenol]]), [[iloprost]], [[treprostinil]], and [[beraprost]] with epoprostenol being favored in some studies.<ref name="pmid26438636"/><ref name="pmid26825901">{{cite journal | vauthors = Zhang H, Li X, Huang J, Li H, Su Z, Wang J | title = Comparative Efficacy and Safety of Prostacyclin Analogs for Pulmonary Arterial Hypertension: A Network Meta-Analysis | journal = Medicine | volume = 95 | issue = 4 | pages = e2575 | year = 2016 | pmid = 26825901 | pmc = 5291571 | doi = 10.1097/MD.0000000000002575 | url = }}</ref><ref name="pmid28096285">{{cite journal | vauthors = Sitbon O, Vonk Noordegraaf A | title = Epoprostenol and pulmonary arterial hypertension: 20 years of clinical experience | journal = European Respiratory Review | volume = 26 | issue = 143 | pages = | year = 2017 | pmid = 28096285 | doi = 10.1183/16000617.0055-2016 | url = }}</ref> However, newly developed IP agonists with favorable pharmacological features such as [[Selexipag]] have been granted by the [[US FDA]] [[Orphan Drug]] status for the treatment of pulmonary hypertension. IP agonists are also to treat severe vasoconstriction in [[Raynaud's disease]], Raynaud's disease-like syndromes, and [[scleroderma]].<ref name="pmid25673314">{{cite journal | vauthors = Poredos P, Poredos P | title = Raynaud's Syndrome: a neglected disease | journal = International Angiology : a Journal of the International Union of Angiology | volume = 35 | issue = 2 | pages = 117–21 | year = 2016 | pmid = 25673314 | doi = | url = }}</ref><ref name="pmid28018840">{{cite journal | vauthors = Young A, Namas R, Dodge C, Khanna D | title = Hand Impairment in Systemic Sclerosis: Various Manifestations and Currently Available Treatment | journal = Current Treatment Options in Rheumatology | volume = 2 | issue = 3 | pages = 252–269 | year = 2016 | pmid = 28018840 | doi = 10.1007/s40674-016-0052-9 | url = }}</ref> Epoprostenol causes improvements in hemodynamic parameters and oxygenation in patients suffering the [[acute respiratory distress syndrome]] but due to the limited number of randomized clinical trials and  lack of studies investigating mortality, its use cannot be recommended as standard of care for this disease and should be reserved for those refractory to traditional therapies.<ref name="pmid26294418"/> A [[meta-analysis]] of 18 clinical trials on the use of prostanoids including prinicpally IP receptor agonists on patients with sever lower limb peripheral artery disease due to diverse causes found that these drugs may reduce the extent of limb tissue that needed to be amputated.  However, the studies did not support extensive use of prostanoids in patients with critical limb ischemia as an adjunct to revascularization or as an alternative to major amputation in cases which cannot undergo revascularization.<ref name="pmid26516035">{{cite journal | vauthors = Vitale V, Monami M, Mannucci E | title = Prostanoids in patients with peripheral arterial disease: A meta-analysis of placebo-controlled randomized clinical trials | journal = Journal of Diabetes and Its Complications | volume = 30 | issue = 1 | pages = 161–6 | year = 2016 | pmid = 26516035 | doi = 10.1016/j.jdiacomp.2015.09.006 | url = }}</ref>
IP receptor agonists are front-line drugs to treat [[pulmonary hypertension]]. Major drugs in this category include PGI<sub>2</sub> itself (i.e. [[epoprostenol]]), [[iloprost]], [[treprostinil]], and [[beraprost]] with epoprostenol being favored in some studies.<ref name="pmid26438636"/><ref name="pmid26825901">{{cite journal | vauthors = Zhang H, Li X, Huang J, Li H, Su Z, Wang J | title = Comparative Efficacy and Safety of Prostacyclin Analogs for Pulmonary Arterial Hypertension: A Network Meta-Analysis | journal = Medicine | volume = 95 | issue = 4 | pages = e2575 | date = January 2016 | pmid = 26825901 | pmc = 5291571 | doi = 10.1097/MD.0000000000002575 }}</ref><ref name="pmid28096285">{{cite journal | vauthors = Sitbon O, Vonk Noordegraaf A | title = Epoprostenol and pulmonary arterial hypertension: 20 years of clinical experience | journal = European Respiratory Review | volume = 26 | issue = 143 | date = January 2017 | pmid = 28096285 | doi = 10.1183/16000617.0055-2016 | page=160055}}</ref> However, newly developed IP agonists with favorable pharmacological features such as [[Selexipag]] have been granted by the [[US FDA]] [[Orphan Drug]] status for the treatment of pulmonary hypertension. IP agonists are also to treat severe vasoconstriction in [[Raynaud's disease]], Raynaud's disease-like syndromes, and [[scleroderma]].<ref name="pmid25673314">{{cite journal | vauthors = Poredos P, Poredos P | title = Raynaud's Syndrome: a neglected disease | journal = International Angiology | volume = 35 | issue = 2 | pages = 117–21 | date = April 2016 | pmid = 25673314 | doi = }}</ref><ref name="pmid28018840">{{cite journal | vauthors = Young A, Namas R, Dodge C, Khanna D | title = Hand Impairment in Systemic Sclerosis: Various Manifestations and Currently Available Treatment | journal = Current Treatment Options in Rheumatology | volume = 2 | issue = 3 | pages = 252–269 | date = September 2016 | pmid = 28018840 | doi = 10.1007/s40674-016-0052-9 | pmc=5176259}}</ref> Epoprostenol causes improvements in hemodynamic parameters and oxygenation in patients suffering the [[acute respiratory distress syndrome]] but due to the limited number of randomized clinical trials and  lack of studies investigating mortality, its use cannot be recommended as standard of care for this disease and should be reserved for those refractory to traditional therapies.<ref name="pmid26294418"/> A [[meta-analysis]] of 18 clinical trials on the use of prostanoids including prinicpally IP receptor agonists on patients with sever lower limb peripheral artery disease due to diverse causes found that these drugs may reduce the extent of limb tissue that needed to be amputated.  However, the studies did not support extensive use of prostanoids in patients with critical limb ischemia as an adjunct to revascularization or as an alternative to major amputation in cases which cannot undergo revascularization.<ref name="pmid26516035">{{cite journal | vauthors = Vitale V, Monami M, Mannucci E | title = Prostanoids in patients with peripheral arterial disease: A meta-analysis of placebo-controlled randomized clinical trials | journal = Journal of Diabetes and Its Complications | volume = 30 | issue = 1 | pages = 161–6 | year = 2016 | pmid = 26516035 | doi = 10.1016/j.jdiacomp.2015.09.006 }}</ref>


=== Thrombotic diseases ===
=== Thrombotic diseases ===
IP receptor agonists have been used to treat [[Thromboangiitis obliterans]], a disease involving blood clotting and inflammation of the small and medium-sized arteries and veins in the hands and feet.<ref name="pmid26967103">{{cite journal | vauthors = Cacione DG, Macedo CR, Baptista-Silva JC | title = Pharmacological treatment for Buerger's disease | journal = The Cochrane Database of Systematic Reviews | volume = 3 | issue = | pages = CD011033 | year = 2016 | pmid = 26967103 | doi = 10.1002/14651858.CD011033.pub3 | url = }}</ref>
IP receptor agonists have been used to treat [[Thromboangiitis obliterans]], a disease involving blood clotting and inflammation of the small and medium-sized arteries and veins in the hands and feet.<ref name="pmid26967103">{{cite journal | vauthors = Cacione DG, Macedo CR, Baptista-Silva JC | title = Pharmacological treatment for Buerger's disease | journal = The Cochrane Database of Systematic Reviews | volume = 3 | issue = | pages = CD011033 | date = March 2016 | pmid = 26967103 | doi = 10.1002/14651858.CD011033.pub3 }}</ref>


=== Genomic studies ===
=== Genomic studies ===
An adenine (A) to cytosine (C) [[synonymous substitution]] at base 984 (i.e. A984C) in exon 3 of ''PTGIR''' is the most frequent [[single nucleotide polymorphism]] (SNP) variant in a sampling of Japanese. This variant was associated with an increase in platelet activation responses in vitro and an increase in incidence of [[cerebral ischemia]]. Two other synonymous SNP variants, V53V and S328S, in ''PTGIR'' in an Italian population study were associated with enhanced platelet activation response and deep vein thrombosis.<ref name="pmid27708579">{{cite journal | vauthors = Cornejo-García JA, Perkins JR, Jurado-Escobar R, García-Martín E, Agúndez JA, Viguera E, Pérez-Sánchez N, Blanca-López N | title = Pharmacogenomics of Prostaglandin and Leukotriene Receptors | journal = Frontiers in Pharmacology | volume = 7 | issue = | pages = 316 | year = 2016 | pmid = 27708579 | pmc = 5030812 | doi = 10.3389/fphar.2016.00316 | url = }}</ref> The rare SNP variant 795C of 794T in the ''PTGIR'' gene is associated with an increased incidence of [[Aspirin-induced asthma]] and a greater percentage fall in the [[forced expiratory volume]] response of airways to inhalation of an aspirin like compound (lysine-acetyl salicylic acid) in a Korean population sample.<ref name="pmid15898979">{{cite journal | vauthors = Kim SH, Choi JH, Park HS, Holloway JW, Lee SK, Park CS, Shin HD | title = Association of thromboxane A2 receptor gene polymorphism with the phenotype of acetyl salicylic acid-intolerant asthma | journal = Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology | volume = 35 | issue = 5 | pages = 585–90 | year = 2005 | pmid = 15898979 | doi = 10.1111/j.1365-2222.2005.02220.x | url = }}</ref><ref name="pmid27990118">{{cite journal | vauthors = Thompson MD, Capra V, Clunes MT, Rovati GE, Stankova J, Maj MC, Duffy DL | title = Cysteinyl Leukotrienes Pathway Genes, Atopic Asthma and Drug Response: From Population Isolates to Large Genome-Wide Association Studies | journal = Frontiers in Pharmacology | volume = 7 | issue = | pages = 299 | year = 2016 | pmid = 27990118 | pmc = 5131607 | doi = 10.3389/fphar.2016.00299 | url = }}</ref>
An adenine (A) to cytosine (C) [[synonymous substitution]] at base 984 (i.e. A984C) in exon 3 of ''PTGIR''' is the most frequent [[single nucleotide polymorphism]] (SNP) variant in a sampling of Japanese. This variant was associated with an increase in platelet activation responses in vitro and an increase in incidence of [[cerebral ischemia]]. Two other synonymous SNP variants, V53V and S328S, in ''PTGIR'' in an Italian population study were associated with enhanced platelet activation response and deep vein thrombosis.<ref name="pmid27708579">{{cite journal | vauthors = Cornejo-García JA, Perkins JR, Jurado-Escobar R, García-Martín E, Agúndez JA, Viguera E, Pérez-Sánchez N, Blanca-López N | title = Pharmacogenomics of Prostaglandin and Leukotriene Receptors | journal = Frontiers in Pharmacology | volume = 7 | issue = | pages = 316 | year = 2016 | pmid = 27708579 | pmc = 5030812 | doi = 10.3389/fphar.2016.00316 }}</ref> The rare SNP variant 795C of 794T in the ''PTGIR'' gene is associated with an increased incidence of [[Aspirin-induced asthma]] and a greater percentage fall in the [[forced expiratory volume]] response of airways to inhalation of an aspirin like compound (lysine-acetyl salicylic acid) in a Korean population sample.<ref name="pmid15898979">{{cite journal | vauthors = Kim SH, Choi JH, Park HS, Holloway JW, Lee SK, Park CS, Shin HD | title = Association of thromboxane A2 receptor gene polymorphism with the phenotype of acetyl salicylic acid-intolerant asthma | journal = Clinical and Experimental Allergy | volume = 35 | issue = 5 | pages = 585–90 | date = May 2005 | pmid = 15898979 | doi = 10.1111/j.1365-2222.2005.02220.x }}</ref><ref name="pmid27990118">{{cite journal | vauthors = Thompson MD, Capra V, Clunes MT, Rovati GE, Stankova J, Maj MC, Duffy DL | title = Cysteinyl Leukotrienes Pathway Genes, Atopic Asthma and Drug Response: From Population Isolates to Large Genome-Wide Association Studies | journal = Frontiers in Pharmacology | volume = 7 | issue = | pages = 299 | year = 2016 | pmid = 27990118 | pmc = 5131607 | doi = 10.3389/fphar.2016.00299 }}</ref>


==See also==
== See also ==
* ''PTGIR'' gene (https://www.wikigenes.org/e/gene/e/5739.html)
* ''PTGIR'' gene (https://www.wikigenes.org/e/gene/e/5739.html)
* [[Prostacyclin|PGI<sub>2</sub>]]
* [[Prostacyclin|PGI<sub>2</sub>]]
Line 62: Line 60:
* [[Eicosanoid receptor]]
* [[Eicosanoid receptor]]


==References==
== References ==
{{reflist|30em}}
{{reflist|30em}}


==Further reading==
== Further reading ==
{{Refbegin|35em}}
{{Refbegin|35em}}
{{PBB_Further_reading
* {{cite journal | vauthors = Coleman RA, Smith WL, Narumiya S | title = International Union of Pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes | journal = Pharmacological Reviews | volume = 46 | issue = 2 | pages = 205–29 | date = June 1994 | pmid = 7938166 | doi =  }}
| citations =
* {{cite journal | vauthors = Rauvala H, Peng HB | title = HB-GAM (heparin-binding growth-associated molecule) and heparin-type glycans in the development and plasticity of neuron-target contacts | journal = Progress in Neurobiology | volume = 52 | issue = 2 | pages = 127–44 | date = June 1997 | pmid = 9185236 | doi = 10.1016/S0301-0082(97)00007-5 }}
*{{cite journal |vauthors=Coleman RA, Smith WL, Narumiya S |title=International Union of Pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes |journal=Pharmacol. Rev. |volume=46 |issue= 2 |pages= 205–29 |year= 1994 |pmid= 7938166 |doi=  }}
* {{cite journal | vauthors = Smyth EM, FitzGerald GA | title = Human prostacyclin receptor | journal = Vitamins and Hormones | volume = 65 | issue =  | pages = 149–65 | year = 2003 | pmid = 12481546 | doi = 10.1016/S0083-6729(02)65063-0 | isbn = 978-0-12-709865-4 | series = Vitamins & Hormones }}
*{{cite journal |vauthors=Rauvala H, Peng HB |title=HB-GAM (heparin-binding growth-associated molecule) and heparin-type glycans in the development and plasticity of neuron-target contacts |journal=Prog. Neurobiol. |volume=52 |issue= 2 |pages= 127–44 |year= 1997 |pmid= 9185236 |doi=10.1016/S0301-0082(97)00007-5 }}
* {{cite journal | vauthors = Boie Y, Rushmore TH, Darmon-Goodwin A, Grygorczyk R, Slipetz DM, Metters KM, Abramovitz M | title = Cloning and expression of a cDNA for the human prostanoid IP receptor | journal = The Journal of Biological Chemistry | volume = 269 | issue = 16 | pages = 12173–8 | date = April 1994 | pmid = 7512962 | doi =  }}
*{{cite journal |vauthors=Smyth EM, FitzGerald GA |title=Human prostacyclin receptor |journal=Vitam. Horm. |volume=65 |issue=  |pages= 149–65 |year= 2003 |pmid= 12481546 |doi=10.1016/S0083-6729(02)65063-0 | series=Vitamins & Hormones  | isbn=978-0-12-709865-4 }}
* {{cite journal | vauthors = Katsuyama M, Sugimoto Y, Namba T, Irie A, Negishi M, Narumiya S, Ichikawa A | title = Cloning and expression of a cDNA for the human prostacyclin receptor | journal = FEBS Letters | volume = 344 | issue = 1 | pages = 74–8 | date = May 1994 | pmid = 7514139 | doi = 10.1016/0014-5793(94)00355-6 }}
*{{cite journal   |vauthors=Boie Y, Rushmore TH, Darmon-Goodwin A, etal |title=Cloning and expression of a cDNA for the human prostanoid IP receptor |journal=J. Biol. Chem. |volume=269 |issue= 16 |pages= 12173–8 |year= 1994 |pmid= 7512962 |doi=  }}
* {{cite journal | vauthors = Ogawa Y, Tanaka I, Inoue M, Yoshitake Y, Isse N, Nakagawa O, Usui T, Itoh H, Yoshimasa T, Narumiya S | title = Structural organization and chromosomal assignment of the human prostacyclin receptor gene | journal = Genomics | volume = 27 | issue = 1 | pages = 142–8 | date = May 1995 | pmid = 7665161 | doi = 10.1006/geno.1995.1016 }}
*{{cite journal   |vauthors=Katsuyama M, Sugimoto Y, Namba T, etal |title=Cloning and expression of a cDNA for the human prostacyclin receptor |journal=FEBS Lett. |volume=344 |issue= 1 |pages= 74–8 |year= 1994 |pmid= 7514139 |doi=10.1016/0014-5793(94)00355-6 }}
* {{cite journal | vauthors = Duncan AM, Anderson LL, Funk CD, Abramovitz M, Adam M | title = Chromosomal localization of the human prostanoid receptor gene family | journal = Genomics | volume = 25 | issue = 3 | pages = 740–2 | date = February 1995 | pmid = 7759114 | doi = 10.1016/0888-7543(95)80022-E }}
*{{cite journal   |vauthors=Ogawa Y, Tanaka I, Inoue M, etal |title=Structural organization and chromosomal assignment of the human prostacyclin receptor gene |journal=Genomics |volume=27 |issue= 1 |pages= 142–8 |year= 1995 |pmid= 7665161 |doi=10.1006/geno.1995.1016 }}
* {{cite journal | vauthors = Nakagawa O, Tanaka I, Usui T, Harada M, Sasaki Y, Itoh H, Yoshimasa T, Namba T, Narumiya S, Nakao K | title = Molecular cloning of human prostacyclin receptor cDNA and its gene expression in the cardiovascular system | journal = Circulation | volume = 90 | issue = 4 | pages = 1643–7 | date = October 1994 | pmid = 7923647 | doi = 10.1161/01.cir.90.4.1643 }}
*{{cite journal   |vauthors=Duncan AM, Anderson LL, Funk CD, etal |title=Chromosomal localization of the human prostanoid receptor gene family |journal=Genomics |volume=25 |issue= 3 |pages= 740–2 |year= 1995 |pmid= 7759114 |doi=10.1016/0888-7543(95)80022-E }}
* {{cite journal | vauthors = Bonaldo MF, Lennon G, Soares MB | title = Normalization and subtraction: two approaches to facilitate gene discovery | journal = Genome Research | volume = 6 | issue = 9 | pages = 791–806 | date = September 1996 | pmid = 8889548 | doi = 10.1101/gr.6.9.791 }}
*{{cite journal   |vauthors=Nakagawa O, Tanaka I, Usui T, etal |title=Molecular cloning of human prostacyclin receptor cDNA and its gene expression in the cardiovascular system |journal=Circulation |volume=90 |issue= 4 |pages= 1643–7 |year= 1994 |pmid= 7923647 |doi= 10.1161/01.cir.90.4.1643}}
* {{cite journal | vauthors = Sasaki Y, Takahashi T, Tanaka I, Nakamura K, Okuno Y, Nakagawa O, Narumiya S, Nakao K | title = Expression of prostacyclin receptor in human megakaryocytes | journal = Blood | volume = 90 | issue = 3 | pages = 1039–46 | date = August 1997 | pmid = 9242534 | doi =  }}
*{{cite journal |vauthors=Bonaldo MF, Lennon G, Soares MB |title=Normalization and subtraction: two approaches to facilitate gene discovery |journal=Genome Res. |volume=6 |issue= 9 |pages= 791–806 |year= 1997 |pmid= 8889548 |doi=10.1101/gr.6.9.791 }}
* {{cite journal | vauthors = Fisch A, Tobusch K, Veit K, Meyer J, Darius H | title = Prostacyclin receptor desensitization is a reversible phenomenon in human platelets | journal = Circulation | volume = 96 | issue = 3 | pages = 756–60 | date = August 1997 | pmid = 9264479 | doi = 10.1161/01.cir.96.3.756 }}
*{{cite journal   |vauthors=Sasaki Y, Takahashi T, Tanaka I, etal |title=Expression of prostacyclin receptor in human megakaryocytes |journal=Blood |volume=90 |issue= 3 |pages= 1039–46 |year= 1997 |pmid= 9242534 |doi=  }}
* {{cite journal | vauthors = Smyth EM, Li WH, FitzGerald GA | title = Phosphorylation of the prostacyclin receptor during homologous desensitization. A critical role for protein kinase c | journal = The Journal of Biological Chemistry | volume = 273 | issue = 36 | pages = 23258–66 | date = September 1998 | pmid = 9722557 | doi = 10.1074/jbc.273.36.23258 }}
*{{cite journal   |vauthors=Fisch A, Tobusch K, Veit K, etal |title=Prostacyclin receptor desensitization is a reversible phenomenon in human platelets |journal=Circulation |volume=96 |issue= 3 |pages= 756–60 |year= 1997 |pmid= 9264479 |doi= 10.1161/01.cir.96.3.756}}
* {{cite journal | vauthors = Kömhoff M, Lesener B, Nakao K, Seyberth HW, Nüsing RM | title = Localization of the prostacyclin receptor in human kidney | journal = Kidney International | volume = 54 | issue = 6 | pages = 1899–908 | date = December 1998 | pmid = 9853255 | doi = 10.1046/j.1523-1755.1998.00213.x }}
*{{cite journal |vauthors=Smyth EM, Li WH, FitzGerald GA |title=Phosphorylation of the prostacyclin receptor during homologous desensitization. A critical role for protein kinase c |journal=J. Biol. Chem. |volume=273 |issue= 36 |pages= 23258–66 |year= 1998 |pmid= 9722557 |doi=10.1074/jbc.273.36.23258 }}
* {{cite journal | vauthors = Hayes JS, Lawler OA, Walsh MT, Kinsella BT | title = The prostacyclin receptor is isoprenylated. Isoprenylation is required for efficient receptor-effector coupling | journal = The Journal of Biological Chemistry | volume = 274 | issue = 34 | pages = 23707–18 | date = August 1999 | pmid = 10446129 | doi = 10.1074/jbc.274.34.23707 }}
*{{cite journal   |vauthors=Kömhoff M, Lesener B, Nakao K, etal |title=Localization of the prostacyclin receptor in human kidney |journal=Kidney Int. |volume=54 |issue= 6 |pages= 1899–908 |year= 1999 |pmid= 9853255 |doi= 10.1046/j.1523-1755.1998.00213.x }}
* {{cite journal | vauthors = Smyth EM, Austin SC, Reilly MP, FitzGerald GA | title = Internalization and sequestration of the human prostacyclin receptor | journal = The Journal of Biological Chemistry | volume = 275 | issue = 41 | pages = 32037–45 | date = October 2000 | pmid = 10889200 | doi = 10.1074/jbc.M003873200 }}
*{{cite journal |vauthors=Hayes JS, Lawler OA, Walsh MT, Kinsella BT |title=The prostacyclin receptor is isoprenylated. Isoprenylation is required for efficient receptor-effector coupling |journal=J. Biol. Chem. |volume=274 |issue= 34 |pages= 23707–18 |year= 1999 |pmid= 10446129 |doi=10.1074/jbc.274.34.23707 }}
* {{cite journal | vauthors = Lawler OA, Miggin SM, Kinsella BT | title = Protein kinase A-mediated phosphorylation of serine 357 of the mouse prostacyclin receptor regulates its coupling to G(s)-, to G(i)-, and to G(q)-coupled effector signaling | journal = The Journal of Biological Chemistry | volume = 276 | issue = 36 | pages = 33596–607 | date = September 2001 | pmid = 11443126 | doi = 10.1074/jbc.M104434200 }}
*{{cite journal |vauthors=Smyth EM, Austin SC, Reilly MP, FitzGerald GA |title=Internalization and sequestration of the human prostacyclin receptor |journal=J. Biol. Chem. |volume=275 |issue= 41 |pages= 32037–45 |year= 2000 |pmid= 10889200 |doi= 10.1074/jbc.M003873200 }}
* {{cite journal | vauthors = Zhang Z, Austin SC, Smyth EM | title = Glycosylation of the human prostacyclin receptor: role in ligand binding and signal transduction | journal = Molecular Pharmacology | volume = 60 | issue = 3 | pages = 480–7 | date = September 2001 | pmid = 11502878 | doi =  }}
*{{cite journal |vauthors=Lawler OA, Miggin SM, Kinsella BT |title=Protein kinase A-mediated phosphorylation of serine 357 of the mouse prostacyclin receptor regulates its coupling to G(s)-, to G(i)-, and to G(q)-coupled effector signaling |journal=J. Biol. Chem. |volume=276 |issue= 36 |pages= 33596–607 |year= 2001 |pmid= 11443126 |doi= 10.1074/jbc.M104434200 }}
* {{cite journal | vauthors = Fortier I, Patry C, Lora M, Samadfan R, de Brum-Fernandes AJ | title = Immunohistochemical localization of the prostacyclin receptor (IP) human bone | journal = Prostaglandins, Leukotrienes, and Essential Fatty Acids | volume = 65 | issue = 2 | pages = 79–83 | date = August 2001 | pmid = 11545623 | doi = 10.1054/plef.2001.0292 }}
*{{cite journal |vauthors=Zhang Z, Austin SC, Smyth EM |title=Glycosylation of the human prostacyclin receptor: role in ligand binding and signal transduction |journal=Mol. Pharmacol. |volume=60 |issue= 3 |pages= 480–7 |year= 2001 |pmid= 11502878 |doi=  }}
*{{cite journal   |vauthors=Fortier I, Patry C, Lora M, etal |title=Immunohistochemical localization of the prostacyclin receptor (IP) human bone |journal=Prostaglandins Leukot. Essent. Fatty Acids |volume=65 |issue= 2 |pages= 79–83 |year= 2001 |pmid= 11545623 |doi= 10.1054/plef.2001.0292 }}
}}
{{Refend}}
{{Refend}}


==External links==
== External links ==
*{{cite web | url = http://www.iuphar-db.org/GPCR/ReceptorDisplayForward?receptorID=2426 | title = Prostanoid Receptors: IP<sub>1</sub> | accessdate = | date = | format = | work = IUPHAR Database of Receptors and Ion Channels | publisher = International Union of Basic and Clinical Pharmacology}}
* {{cite web | url = http://www.iuphar-db.org/GPCR/ReceptorDisplayForward?receptorID=2426 | title = Prostanoid Receptors: IP<sub>1</sub> | accessdate = | date = | format = | work = IUPHAR Database of Receptors and Ion Channels | publisher = International Union of Basic and Clinical Pharmacology}}


{{NLM content}}
{{NLM content}}
{{G protein-coupled receptors}}
{{G protein-coupled receptors}}
{{Prostanoidergics}}
{{Prostanoidergics}}
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[[Category:G protein coupled receptors]]
[[Category:G protein-coupled receptors]]

Latest revision as of 18:01, 24 September 2018

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The Prostacyclin receptor , also termed the prostaglandin I2 receptor or just IP, is a receptor belonging to the prostaglandin (PG) group of receptors. IP binds to and mediates the biological actions of prostacyclin (also termed Prostaglandin I2, PGI2, or when used as a drug, epoprostenol). IP is encoded in humans by the PTGIR gene. While possessing many functions as defined in animal model studies, the major clinical relevancy of IP is as a powerful vasodilator: stimulators of IP are used to treat severe and even life-threatening diseases involving pathological vasoconstriction.

Gene

The PTGIR gene is located on human chromosome 19 at position q13.32 (i.e. 19q13.32), contains 6 exons, and codes for a G protein coupled receptor (GPCR) of the rhodopsin-like receptor family, Subfamily A14 (see rhodopsin-like receptors#Subfamily A14).[1]

Expression

IP is most highly expressed in brain and thymus and is readily detected in most other tissues. It is found throughout the vascular network on endothelium and smooth muscle cells.[1][2]

Ligands

Activating ligands

Standard prostanoids have the following relative efficacies as receptor ligands in binding to and activating IP: PGI2>>PGD2=PGE2=PGF2α>TXA2. In typical binding studies, PGI2 has one-half of its maximal binding capacity and cell-stimulating actions at ~1 nanomolar whereas the other prostaglandins are >50-fold to 100-fold weaker than this. However, PGI2 is very unstable, spontaneously converting to a far less active derivative 6-keto-PGF1 alpha within 1 minute of its formation. This instability makes defining the exact affinity of PGI2 for IP difficult. It also makes it important to have stable synthetic analogs of PGI2 for clinical usage. The most potent of these receptor agonists for binding to and activating IP are iloprost, taprostene, and esuberaprost which have Kd values (i.e. concentrations which bind to half of available IP receptors) in the low nanamole/liter range (http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=345/).[3]

Inhibiting ligands

Several synthetic compounds bind to, but do not activate, IP and thereby inhibit its activation by the activating ligands just described. These receptor antagonists include RO1138452, RO3244794, TG6-129, and BAY-73-1449, all of which have Kd values for IP at or beneath low nanomol/liter levels (http://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=345/).

Mechanism of cell activation

IP is classified as a relaxant type of prostenoid receptor based on its ability, upon activation, to relax certain pre-contracted smooth muscle preparations and smooth muscle-containing tissues such as those of pulmonary arteries and veins.[4] When bound to PGI2 or other of its agonists, IP stimulates one or more of three types of G protein complexes, depending on cell type: a) Gs alpha subunit-Gβγ complexes which release Gs that then stimulates adenyl cyclase to raise intracellular levels of cAMP and thereby activate cAMP-regulated protein kinases A-dependent cell signaling pathways (see PKA); b) Gq alpha subunit-Gβγ complexes which release Gq that then stimulates other cell signaling pathways (e.g. phospholipase C/IP3/cell Ca2+ mobilization/diacylglycerol/protein kinase Cs, calmodulin-modulated myosin light chain kinase, RAF/MEK/Mitogen-activated protein kinases, PKC/Ca2+/Calcineurin/Nuclear factor of activated T-cells; and EGF cellular receptors; and c) Gi alpha subunit-Giβγ) complexes which releases Gi that then simulates phospholipase C to cleave phosphatidylinositol triphosphate into inositol triphosphate that raises intracellular CaCa2 levels thereby regulating Calcium signaling pathways and diacylglycerol that activates certain protein kinase C enzymes )that phosphorylate and thereby regulate target proteins involved in cell signaling (see Protein kinase C#Function). Studies suggest that stimulation of Gsβγ complexes is required for activation of the Gqβγ- and Giβγ-dependent pathways.[3][5][6][7] In certain cells, activation of FP also stimulates G12/G13-Gβγ G proteins to activate the Rho family of GTPases signaling proteins and Gi-Gβγ G proteins to activateRaf/MEK/mitogen-activated kinase pathways.

Functions

Studies using animals genetically engineered to lack FP and examining the actions of EP4 receptor agonists in animals as well as animal and human tissues indicate that this receptor serves various functions. It has been regarded as the most successful therapeutic target among the 9 prostanoid receptors.[6]

Platelets

IP gene knockout mice (i.e. IP(-/-) mice) exhibit increased tendency to thrombosis in response to experimentally-induced Endothelium, a result which appears to reflect, at least in part, the loss of IP's anti-platelet activity.[8][9] IP activation of animal and human platelets inhibits their aggreatation response and as one consequence of this inhibition of platelet-dependent blood clotting. The PGI2-IP axis along with the production of nitric oxide, acting together additively and potentially synergistically, are powerful and physiological negative regulators of platelet function and thereby blood clotting in humans. Studies suggest that the 2-IP axis is impaired in patients with a tendency to develop pathological thrombosis such as occurs in obesity, diabetes, and coronary artery disease.[6][10]

Cardiovascular system

IP activation stimulates the dilation of arteries and veins in various animal models as well as in humans. It increases the blood flow through, for example, the pulmonary, coronary, retinal and choroid circulation. Inhaled PGI2 causes a modest fall in diastolic and small fall in systolic blood pressure in humans. This action involves IP's ability to relax vascular smooth muscle and is considered to be one of the fundamental functions of IP receptors. Furthermore, IP(-/-) mice on a high salt diet develop significantly higher levels of hypertension, cardiac fibrosis, and cardiac hypertrophy than control mice. The vasodilating and, perhaps, platelet-inhibiting effects of IP receptors likely underlie its ability suppress hypertension and protect tissues such as the heart in this model as well as the heart, brain, and gastrointestinal tract in various animal models of ischemic injury.[6] Indeed, IP agonists are used to treat patients pathological vasoconstriction diseases.[11] The injection of IP activators into the skin of rodents increases local capillary permeability and swelling; IP(-/-) mice fail to show this increased capillary permeability and swelling in response not only to IP activators but also in a model of carrageenan- or bradykinin-induced paw edema. IP antagonists likewise reduce experimentally-induced capillary permeability and swelling in rats. This actions is also considered a physiological function of IP receptors,[3][6] but can contribute to the toxicity of IP activators in patients by inducing, for example, life-threatening pulmonary edema.[11]

IP activators inhibit the adherence of circulating platelets and leukocytes adherence to vascular endothelium thereby blocking their entry into sites of tissue disturbance. The activators also inhibit vascular smooth muscle cells from proliferation by blocking these cells' growth cycle and triggering their apoptosis (i.e. cell death]]. These actions, along with its anti-inflammatory effects, may underlie the ability of IP gene knockout in an ApoE(−/−) mosue model to cause an accelerated rate of developing atherosclerosis.[3] [6]

Inflammation

Mouse studies indicate that the PGI2-IP axis activates cellular signaling pathways that tend to suppress allergic inflammation. The axis inhibits bone marrow-derived dendritic cells (i.e. antigen-presenting cells that process antigen material, present it on their surfaces for delivery to T cells, and otherwise regulate innate and adaptive immune system responses) from producing pro-inflammatory cytokines (e.g. IL-12, TNF-alpha, IL-1-alpha, and IL-6) while stimulating them to increase production of the anti-inflammatory cytokine, IL-10. IP receptor activation of these cells also blocks their lipopolysaccharide-stimulated expression of pro-inflammatory cell surface proteins (i.e. CD86, CD40, and MHC class II molecules) that are critical for developing adaptive immune responses. IL receptor-activated bone marrow-derived dendritic cells showed a greatly reduced ability to stimulate the proliferation of T helper cell as well as the ability of these cells to produce pro-allergic cytokines (i.e. IL-5 and IL-13)s. In a mouse model of allergic inflammation, PGI2 reduced the maturation and migration of lung mature dendritic cells to Mediastinal lymph nodes while increasing the egress of immature dendritic cells away from the lung. These effects resulted in a decrease in allergen-induced responses of the cells mediating allergic reactivity, TH-2 cells. These IP-induced responses likely contribute to its apparent function in inhibiting certain mouse inflammation responses as exemplified by the failure of IP receptor deficient mice to develop full lung airway allergic responses to ovalbumin in a model of allergic inflammation.[3][2]

In human studies, PGI2 failed to alter bronchoconstriction responses to allergen but did protect against exercise-induced and ultrasonic water-induced bronchoconstriction in asthmatic patients. It also caused bronchodilation in two asthmatic patients. However, these studies were done before the availability of potent and selective IP agonists. These agonists might produce more effective inhibitor results on airways allergic diseases but their toxicity (e.g. pulmonary edema, hypotension) has tended to restrict there study in asthmatic patients.[2]

IP receptors also appear involved in suppressing non-allergic inflammatory responses. IP receptor-deficient mice exhibit a reduction in the extent and progression of inflammation in a model of collagen-induced arthritis. This effect may result from regulating the expression of arthritis-related, pro-inflammatory genes (i.e. those for IL-6, VEGF-A, and RANKL).[4][6] On the other hand, IP receptors may serve to promote non-allergic inflammatory responses: IP receptor-deficient mice exhibited increased lung inflammation in a model of bleomycin-induced pulmonary fibrosis while mice made to over-express the PGI2-forming enzyme, Prostacyclin synthase, in their airway epithelial cells were protected against lung injury in this model.[2]

Pain perception

IP(-/-) mice exhibit little or no writhing responses in an acetic acid-induced pain model. The mouse IP receptor also appears to be involved in the development of heat-induced hyperalgesia. These and further studies using IP receptor antagonists in rats indicate that IP receptors on pain-perceiving sensory neurons of the dorsal root ganglia as well as on certain neurons in the spinal cord transmit signals for pain, particularly pain triggered by inflammation.[3][6]

Clinical significance

Toxicity

IP receptor agonists, particularly when used intravenously, have been associated with the rapid development of pulmonary edema, hypotension, bleeding due to inhibition of platelet aggregation, and tachycardia.[12][13] Clinical use of these agonists is contraindicated in patients suffering many conditions. For example, the IP agonist iloprost is contraindicated in patients with unstable angina; decompensated cardiac failure (unless under close medical supervision); severe cardiac arrhythmias; congenital or acquired heart valve defects; increased risk of bleeding; a history of myocardial infarction in the past 6 months; or a history of cerebrovascular events (e.g. stroke) within 3 months.

Vasoconstriction

IP receptor agonists are front-line drugs to treat pulmonary hypertension. Major drugs in this category include PGI2 itself (i.e. epoprostenol), iloprost, treprostinil, and beraprost with epoprostenol being favored in some studies.[12][14][15] However, newly developed IP agonists with favorable pharmacological features such as Selexipag have been granted by the US FDA Orphan Drug status for the treatment of pulmonary hypertension. IP agonists are also to treat severe vasoconstriction in Raynaud's disease, Raynaud's disease-like syndromes, and scleroderma.[16][17] Epoprostenol causes improvements in hemodynamic parameters and oxygenation in patients suffering the acute respiratory distress syndrome but due to the limited number of randomized clinical trials and lack of studies investigating mortality, its use cannot be recommended as standard of care for this disease and should be reserved for those refractory to traditional therapies.[13] A meta-analysis of 18 clinical trials on the use of prostanoids including prinicpally IP receptor agonists on patients with sever lower limb peripheral artery disease due to diverse causes found that these drugs may reduce the extent of limb tissue that needed to be amputated. However, the studies did not support extensive use of prostanoids in patients with critical limb ischemia as an adjunct to revascularization or as an alternative to major amputation in cases which cannot undergo revascularization.[18]

Thrombotic diseases

IP receptor agonists have been used to treat Thromboangiitis obliterans, a disease involving blood clotting and inflammation of the small and medium-sized arteries and veins in the hands and feet.[19]

Genomic studies

An adenine (A) to cytosine (C) synonymous substitution at base 984 (i.e. A984C) in exon 3 of PTGIR' is the most frequent single nucleotide polymorphism (SNP) variant in a sampling of Japanese. This variant was associated with an increase in platelet activation responses in vitro and an increase in incidence of cerebral ischemia. Two other synonymous SNP variants, V53V and S328S, in PTGIR in an Italian population study were associated with enhanced platelet activation response and deep vein thrombosis.[20] The rare SNP variant 795C of 794T in the PTGIR gene is associated with an increased incidence of Aspirin-induced asthma and a greater percentage fall in the forced expiratory volume response of airways to inhalation of an aspirin like compound (lysine-acetyl salicylic acid) in a Korean population sample.[21][22]

See also

References

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Further reading

  • Coleman RA, Smith WL, Narumiya S (June 1994). "International Union of Pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes". Pharmacological Reviews. 46 (2): 205–29. PMID 7938166.
  • Rauvala H, Peng HB (June 1997). "HB-GAM (heparin-binding growth-associated molecule) and heparin-type glycans in the development and plasticity of neuron-target contacts". Progress in Neurobiology. 52 (2): 127–44. doi:10.1016/S0301-0082(97)00007-5. PMID 9185236.
  • Smyth EM, FitzGerald GA (2003). "Human prostacyclin receptor". Vitamins and Hormones. Vitamins & Hormones. 65: 149–65. doi:10.1016/S0083-6729(02)65063-0. ISBN 978-0-12-709865-4. PMID 12481546.
  • Boie Y, Rushmore TH, Darmon-Goodwin A, Grygorczyk R, Slipetz DM, Metters KM, Abramovitz M (April 1994). "Cloning and expression of a cDNA for the human prostanoid IP receptor". The Journal of Biological Chemistry. 269 (16): 12173–8. PMID 7512962.
  • Katsuyama M, Sugimoto Y, Namba T, Irie A, Negishi M, Narumiya S, Ichikawa A (May 1994). "Cloning and expression of a cDNA for the human prostacyclin receptor". FEBS Letters. 344 (1): 74–8. doi:10.1016/0014-5793(94)00355-6. PMID 7514139.
  • Ogawa Y, Tanaka I, Inoue M, Yoshitake Y, Isse N, Nakagawa O, Usui T, Itoh H, Yoshimasa T, Narumiya S (May 1995). "Structural organization and chromosomal assignment of the human prostacyclin receptor gene". Genomics. 27 (1): 142–8. doi:10.1006/geno.1995.1016. PMID 7665161.
  • Duncan AM, Anderson LL, Funk CD, Abramovitz M, Adam M (February 1995). "Chromosomal localization of the human prostanoid receptor gene family". Genomics. 25 (3): 740–2. doi:10.1016/0888-7543(95)80022-E. PMID 7759114.
  • Nakagawa O, Tanaka I, Usui T, Harada M, Sasaki Y, Itoh H, Yoshimasa T, Namba T, Narumiya S, Nakao K (October 1994). "Molecular cloning of human prostacyclin receptor cDNA and its gene expression in the cardiovascular system". Circulation. 90 (4): 1643–7. doi:10.1161/01.cir.90.4.1643. PMID 7923647.
  • Bonaldo MF, Lennon G, Soares MB (September 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
  • Sasaki Y, Takahashi T, Tanaka I, Nakamura K, Okuno Y, Nakagawa O, Narumiya S, Nakao K (August 1997). "Expression of prostacyclin receptor in human megakaryocytes". Blood. 90 (3): 1039–46. PMID 9242534.
  • Fisch A, Tobusch K, Veit K, Meyer J, Darius H (August 1997). "Prostacyclin receptor desensitization is a reversible phenomenon in human platelets". Circulation. 96 (3): 756–60. doi:10.1161/01.cir.96.3.756. PMID 9264479.
  • Smyth EM, Li WH, FitzGerald GA (September 1998). "Phosphorylation of the prostacyclin receptor during homologous desensitization. A critical role for protein kinase c". The Journal of Biological Chemistry. 273 (36): 23258–66. doi:10.1074/jbc.273.36.23258. PMID 9722557.
  • Kömhoff M, Lesener B, Nakao K, Seyberth HW, Nüsing RM (December 1998). "Localization of the prostacyclin receptor in human kidney". Kidney International. 54 (6): 1899–908. doi:10.1046/j.1523-1755.1998.00213.x. PMID 9853255.
  • Hayes JS, Lawler OA, Walsh MT, Kinsella BT (August 1999). "The prostacyclin receptor is isoprenylated. Isoprenylation is required for efficient receptor-effector coupling". The Journal of Biological Chemistry. 274 (34): 23707–18. doi:10.1074/jbc.274.34.23707. PMID 10446129.
  • Smyth EM, Austin SC, Reilly MP, FitzGerald GA (October 2000). "Internalization and sequestration of the human prostacyclin receptor". The Journal of Biological Chemistry. 275 (41): 32037–45. doi:10.1074/jbc.M003873200. PMID 10889200.
  • Lawler OA, Miggin SM, Kinsella BT (September 2001). "Protein kinase A-mediated phosphorylation of serine 357 of the mouse prostacyclin receptor regulates its coupling to G(s)-, to G(i)-, and to G(q)-coupled effector signaling". The Journal of Biological Chemistry. 276 (36): 33596–607. doi:10.1074/jbc.M104434200. PMID 11443126.
  • Zhang Z, Austin SC, Smyth EM (September 2001). "Glycosylation of the human prostacyclin receptor: role in ligand binding and signal transduction". Molecular Pharmacology. 60 (3): 480–7. PMID 11502878.
  • Fortier I, Patry C, Lora M, Samadfan R, de Brum-Fernandes AJ (August 2001). "Immunohistochemical localization of the prostacyclin receptor (IP) human bone". Prostaglandins, Leukotrienes, and Essential Fatty Acids. 65 (2): 79–83. doi:10.1054/plef.2001.0292. PMID 11545623.

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.