TAT box gene transcriptions: Difference between revisions

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(Created page with "{{AE}} Henry A. Hoff "Some other ''cis''-acting elements, such as pyrimidine boxes (GGTTTT) and TAT boxes (TATCCAT), are usually present in the vicinity of the GARE sequence...")
 
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==TAT box (Yang) samplings==
{{main|Model samplings}}
Copying a responsive elements consensus sequence TATAAAA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or one between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence TATAAAA (starting with SuccessablesTATY.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
# negative strand, negative direction, looking for TATAAAA, 1, TATAAAA at 2853.
# positive strand, negative direction, looking for TATAAAA, 2, TATAAAA at 222, TATAAAA at 183.
# positive strand, positive direction, looking for TATAAAA, 0.
# negative strand, positive direction, looking for TATAAAA, 0.
# complement, negative strand, negative direction, looking for ATATTTT, 2, ATATTTT at 222, ATATTTT at 183.
# complement, positive strand, negative direction, looking for ATATTTT, 1, ATATTTT at 2853.
# complement, positive strand, positive direction, looking for ATATTTT, 0.
# complement, negative strand, positive direction, looking for ATATTTT, 0.
# inverse complement, negative strand, negative direction, looking for TTTTATA, 2, TTTTATA at 2869, TTTTATA at 1740.
# inverse complement, positive strand, negative direction, looking for TTTTATA, 1, TTTTATA at 219.
# inverse complement, positive strand, positive direction, looking for TTTTATA, 0.
# inverse complement, negative strand, positive direction, looking for TTTTATA, 0.
# inverse negative strand, negative direction, looking for AAAATAT, 1, AAAATAT at 219.
# inverse positive strand, negative direction, looking for AAAATAT, 2, AAAATAT at 2869, AAAATAT at 1740.
# inverse positive strand, positive direction, looking for AAAATAT, 0.
# inverse negative strand, positive direction, looking for AAAATAT, 0.
===TATY UTRs===
{{main|UTR promoter gene transcriptions}}
Negative strand, negative direction: TTTTATA at 2869, TATAAAA at 2853.
===TATY distal promoters===
{{main|Distal promoter gene transcriptions}}
Negative strand, negative direction: TTTTATA at 1740.
Positive strand, negative direction: TATAAAA at 222, TTTTATA at 219, TATAAAA at 183.
==TAT box (Fan) samplings==
{{main|Model samplings}}
Copying a responsive elements consensus sequence TATCCAT and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence TATCCAT (starting with SuccessablesTATF.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
# negative strand, negative direction, looking for TATCCAT, 0.
# positive strand, negative direction, looking for TATCCAT, 0.
# positive strand, positive direction, looking for TATCCAT, 0.
# negative strand, positive direction, looking for TATCCAT, 0.
# complement, negative strand, negative direction, looking for ATAGGTA, 0.
# complement, positive strand, negative direction, looking for ATAGGTA, 0.
# complement, positive strand, positive direction, looking for ATAGGTA, 0.
# complement, negative strand, positive direction, looking for ATAGGTA, 0.
# inverse complement, negative strand, negative direction, looking for ATGGATA, 1, ATGGATA at 2996.
# inverse complement, positive strand, negative direction, looking for ATGGATA, 0.
# inverse complement, positive strand, positive direction, looking for ATGGATA, 0.
# inverse complement, negative strand, positive direction, looking for ATGGATA, 0.
# inverse negative strand, negative direction, looking for TACCTAT, 0.
# inverse positive strand, negative direction, looking for TACCTAT, 1, TACCTAT at 2996.
# inverse positive strand, positive direction, looking for TACCTAT, 0.
# inverse negative strand, positive direction, looking for TACCTAT, 0.
===TATF UTRs===
{{main|UTR promoter gene transcriptions}}
Negative strand, negative direction: ATGGATA at 2996.
==Acknowledgements==
The content on this page was first contributed by: Henry A. Hoff.


==See also==
==See also==
{{div col|colwidth=20em}}
{{div col|colwidth=20em}}
* [[GA responsive complex gene transcription laboratory]]
* [[A1BG gene transcription core promoters]]
* [[A1BG gene transcriptions]]
* [[A1BG regulatory elements and regions]]
* [[A1BG response element gene transcriptions]]
* [[A1BG response element negative results]]
* [[A1BG response element positive results]]
* [[Complex locus A1BG and ZNF497]]
{{Div col end}}
{{Div col end}}


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[[Category:Gene transcription lectures]]
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Revision as of 04:46, 3 March 2021

Associate Editor(s)-in-Chief: Henry A. Hoff

"Some other cis-acting elements, such as pyrimidine boxes (GGTTTT) and TAT boxes (TATCCAT), are usually present in the vicinity of the GARE sequence of genes regulated by GA in cereal aleurone cells (Gubler and Jacobsen 1992; Cercos et al. 1999; Tsuji et al. 2006)."[1]

"Nucleotide and deduced amino acid of HMW-GS gene 1Bx23 [contains the] putative TAT box [TATAAAA] and CCACC sequence [CCAATT and CCAT] under double lines."[2]

"In Proteobacteria this consensus Tat box is best defined as Ser-Arg-Arg-Xaa-Phe-Leu-Lys where the arginine residues are almost invariant, the other amino acids are found at a frequency in excess of 50% and Xaa is a polar amino acid (Berks, 1996; Stanley et al., 2000). The corresponding sequence for plant chloroplast Tat substrates is Arg-Arg-Xaa-Hyd-Leu/Met where Hyd is a hydrophobic amino acid (Peltier et al., 2000). In both cases this Tat or twin-arginine consensus motif is located at the amino-terminal side of the n-region/h-region boundary [...]."[3]

TAT box (Yang) samplings

Copying a responsive elements consensus sequence TATAAAA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or one between ZSCAN22 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence TATAAAA (starting with SuccessablesTATY.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for TATAAAA, 1, TATAAAA at 2853.
  2. positive strand, negative direction, looking for TATAAAA, 2, TATAAAA at 222, TATAAAA at 183.
  3. positive strand, positive direction, looking for TATAAAA, 0.
  4. negative strand, positive direction, looking for TATAAAA, 0.
  5. complement, negative strand, negative direction, looking for ATATTTT, 2, ATATTTT at 222, ATATTTT at 183.
  6. complement, positive strand, negative direction, looking for ATATTTT, 1, ATATTTT at 2853.
  7. complement, positive strand, positive direction, looking for ATATTTT, 0.
  8. complement, negative strand, positive direction, looking for ATATTTT, 0.
  9. inverse complement, negative strand, negative direction, looking for TTTTATA, 2, TTTTATA at 2869, TTTTATA at 1740.
  10. inverse complement, positive strand, negative direction, looking for TTTTATA, 1, TTTTATA at 219.
  11. inverse complement, positive strand, positive direction, looking for TTTTATA, 0.
  12. inverse complement, negative strand, positive direction, looking for TTTTATA, 0.
  13. inverse negative strand, negative direction, looking for AAAATAT, 1, AAAATAT at 219.
  14. inverse positive strand, negative direction, looking for AAAATAT, 2, AAAATAT at 2869, AAAATAT at 1740.
  15. inverse positive strand, positive direction, looking for AAAATAT, 0.
  16. inverse negative strand, positive direction, looking for AAAATAT, 0.

TATY UTRs

Negative strand, negative direction: TTTTATA at 2869, TATAAAA at 2853.

TATY distal promoters

Negative strand, negative direction: TTTTATA at 1740.

Positive strand, negative direction: TATAAAA at 222, TTTTATA at 219, TATAAAA at 183.

TAT box (Fan) samplings

Copying a responsive elements consensus sequence TATCCAT and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence TATCCAT (starting with SuccessablesTATF.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for TATCCAT, 0.
  2. positive strand, negative direction, looking for TATCCAT, 0.
  3. positive strand, positive direction, looking for TATCCAT, 0.
  4. negative strand, positive direction, looking for TATCCAT, 0.
  5. complement, negative strand, negative direction, looking for ATAGGTA, 0.
  6. complement, positive strand, negative direction, looking for ATAGGTA, 0.
  7. complement, positive strand, positive direction, looking for ATAGGTA, 0.
  8. complement, negative strand, positive direction, looking for ATAGGTA, 0.
  9. inverse complement, negative strand, negative direction, looking for ATGGATA, 1, ATGGATA at 2996.
  10. inverse complement, positive strand, negative direction, looking for ATGGATA, 0.
  11. inverse complement, positive strand, positive direction, looking for ATGGATA, 0.
  12. inverse complement, negative strand, positive direction, looking for ATGGATA, 0.
  13. inverse negative strand, negative direction, looking for TACCTAT, 0.
  14. inverse positive strand, negative direction, looking for TACCTAT, 1, TACCTAT at 2996.
  15. inverse positive strand, positive direction, looking for TACCTAT, 0.
  16. inverse negative strand, positive direction, looking for TACCTAT, 0.

TATF UTRs

Negative strand, negative direction: ATGGATA at 2996.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

See also

References

  1. Liu-Min Fan, Xiaoyan Feng, Yu Wang and Xing Wang Deng (2007). "Gibberellin Signal Transduction in Rice". Journal of Integrative Plant Biology. 49 (6): 731−741. doi:10.1111/j.1744-7909.2007.00511.x. Retrieved 16 October 2018.
  2. Zu‐Jun Yang, Guang‐Rong Li, Huan‐Lin Shu, Chang Liu, Juan Feng, Zhi‐Jian Chang and Zheng‐Long Ren (31 August 2006). "Molecular characterization of high molecular weight glutenin subunit allele 1Bx23 in common wheat introduced from hexaploid triticale". Hereditas. 143 (December): 159–166. doi:10.1111/j.2006.0018-0661.01948.x. Retrieved 17 October 2018.
  3. Ben C. Berks, Tracy Palmer and Frank Sargent (2004). "The Tat protein translocation pathway and its role in microbial physiology" (PDF). Advances in Microbial Physiology. 47: 187–254. doi:10.1016/S0065-2911(03)47004-5. Retrieved 17 October 2018.

External links