Vhr1p gene transcriptions

Associate Editor(s)-in-Chief: Henry A. Hoff

"Transcription of the Saccharomyces cerevisiae vitamin H transporter gene VHT1 is enhanced by low extracellular biotin."^[1]

"Vhr1p [is] a transcriptional regulator of VHT1 (VHR1 (YIL056w)".^[1]

"Deletion and linker scanning analyses of the VHT1 promoter revealed the palindromic 18-nucleotide sequence AATCA-N₈-TGAYT as the vitamin H-responsive element."^[1]

Human genes

Gene expressions

Vhr1p upstream activator sequence (UAS) occurs in the promoters of VHT1, BIO5 Saccharomyces cerevisiae genes.^[2] Its inducer is biotin where it responds to low biotin concentrations.^[2]

Interactions

Consensus sequences

AATCA-N₈-TGA(C/T)T is the UAS sequence for the transcription factor Vhr1p.^[2]

Binding site for

Hypotheses

A1BG has no regulatory elements in either promoter.
A1BG is not transcribed by a regulatory element.
No regulatory element participates in the transcription of A1BG.

VHR samplings

Copying a responsive elements consensus sequence AATCA-N₈-TGA(C/T)T and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence AATCA-N₈-TGA(C/T)T (starting with SuccessablesVHR.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

negative strand, negative direction, looking for AATCANNNNNNNNTGA(C/T)T, 0.
positive strand, negative direction, looking for AATCANNNNNNNNTGA(C/T)T, 0.
positive strand, positive direction, looking for AATCANNNNNNNNTGA(C/T)T, 0.
negative strand, positive direction, looking for AATCANNNNNNNNTGA(C/T)T, 0.
complement, negative strand, negative direction, looking for TTAGTNNNNNNNNACT(A/G)A, 0.
complement, positive strand, negative direction, looking for TTAGTNNNNNNNNACT(A/G)A, 0.
complement, positive strand, positive direction, looking for TTAGTNNNNNNNNACT(A/G)A, 0.
complement, negative strand, positive direction, looking for TTAGTNNNNNNNNACT(A/G)A, 0.
inverse complement, negative strand, negative direction, looking for A(A/G)TCANNNNNNNNTGATT, 0.
inverse complement, positive strand, negative direction, looking for A(A/G)TCANNNNNNNNTGATT, 0.
inverse complement, positive strand, positive direction, looking for A(A/G)TCANNNNNNNNTGATT, 0.
inverse complement, negative strand, positive direction, looking for A(A/G)TCANNNNNNNNTGATT, 0.
inverse negative strand, negative direction, looking for T(C/T)AGTNNNNNNNNACTAA, 0.
inverse positive strand, negative direction, looking for T(C/T)AGTNNNNNNNNACTAA, 0.
inverse positive strand, positive direction, looking for T(C/T)AGTNNNNNNNNACTAA, 0.
inverse negative strand, positive direction, looking for T(C/T)AGTNNNNNNNNACTAA, 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

References

↑ ^1.0 ^1.1 ^1.2 Matthias Weider, Agnes Machnik, Franz Klebl and Norbert Sauer (12 May 2006). "Vhr1p, a New Transcription Factor from Budding Yeast, Regulates Biotin-dependent Expression of VHT1 and BIO5". Journal of Biological Chemistry. 281 (19): P13513–P13524. doi:10.1074/jbc.M512158200. Retrieved 8 March 2021.
↑ ^2.0 ^2.1 ^2.2 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

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