Cbf1 regulatory factor gene transcriptions

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Associate Editor(s)-in-Chief: Henry A. Hoff

"Cbf1 is a GRF that binds the palindromic E-box motif (CACGTG) and utilizes DNA shape to discriminate between potential binding sites (Gordan et al. 2013). [...] Like the other GRFs, almost all (88% of 113) in vivo Cbf1-bound promoter sites were also detected in vitro [...]. There were also a substantial number of low occupancy “in vitro-only sites,” typically in ORFs [...]."[1]

Human genes

Consensus sequences

"Previous studies have shown that Cbf1 prefers to bind E-boxes with a “T” at the 5′ end of the E-box (Zhou and O'Shea 2011) [TCACGTG]. This manifests as a specific DNA shape flanking both sides of the palindromic core motif (Gordan et al. 2013). Our PB-exo experiments confirmed these preferences for Cbf1 in DNA sequence (Supplemental Fig. S11E) and DNA shape readout [...]. [...] While the discriminatory DNA shape (and DNA sequence) information at one end of the motif is sufficient to support binding, at the strongly bound Cbf1 motifs it is enriched at both ends ([...] with a “T” on the 5′ and “A” on the 3′ end)."[1]

Likely consensus sequences: (TCACGTGA).[1]

Samplings

Copying the consensus Cbf1: 3'-TCACGTGA-5' and putting the sequence in "⌘F" finds no locations or zero for these sequences respectively between ZSCAN22 or ZNF497 and A1BG as can be found by the computer programs.

See also

References

  1. 1.0 1.1 1.2

External links