Alpha-amylase conserved element gene transcriptions

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Associate Editor(s)-in-Chief: Henry A. Hoff

"A few genes were also noticed with the shoot (GATAatGATG) and root (TGACGTCA) specific elements, cell cycle regulation (CCCAACGGT), seed-specific element (CATGCATG) and alpha-amylase conserved elements (TATCCATCCATCC). The presence of a significant number of the regulatory elements suggests strong participation of the U-box E3 gene family in plant development and responses under abiotic and biotic stress conditions. The data also supports the active involvement of the U-box E3 gene family in hormonal regulatory pathways."[1]

Consensus sequences

TATCCATCCATCC[1]

Hypotheses

  1. A1BG has no A boxes in either promoter.
  2. A1BG is not transcribed by an ATA box.
  3. AGCE1 does not participate in the transcription of A1BG.

Alpha-amylase conserved element samplings

Copying a responsive elements consensus sequence TATCCA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence TATCCATCCATCC (starting with SuccessablesAAC.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for 5'-TATCCATCCATCC-3', 0.
  2. negative strand, positive direction, looking for 5'-TATCCATCCATCC-3', 0.
  3. positive strand, negative direction, looking for 5'-TATCCATCCATCC-3', 0.
  4. positive strand, positive direction, looking for 5'-TATCCATCCATCC-3', 0.
  5. complement, negative strand, negative direction, looking for 5'-ATAGGTAGGTAGG-3', 0.
  6. complement, negative strand, positive direction, looking for 5'-ATAGGTAGGTAGG-3', 0.
  7. complement, positive strand, negative direction, looking for 5'-ATAGGTAGGTAGG-3', 0.
  8. complement, positive strand, positive direction, looking for 5'-ATAGGTAGGTAGG-3', 0.
  9. inverse complement, negative strand, negative direction, looking for 5'-GGATGGATGGATA-3', 0.
  10. inverse complement, negative strand, positive direction, looking for 5'-GGATGGATGGATA-3', 0.
  11. inverse complement, positive strand, negative direction, looking for 5'-GGATGGATGGATA-3', 0.
  12. inverse complement, positive strand, positive direction, looking for 5'-GGATGGATGGATA-3', 0.
  13. inverse negative strand, negative direction, looking for 5'-CCTACCTACCTAT-3', 0.
  14. inverse negative strand, positive direction, looking for 5'-CCTACCTACCTAT-3', 0.
  15. inverse positive strand, negative direction, looking for 5'-CCTACCTACCTAT-3', 0.
  16. inverse positive strand, positive direction, looking for 5'-CCTACCTACCTAT-3', 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

See also

References

  1. 1.0 1.1 Bhaskar Sharma & Joemar Taganna (12 June 2020). "Genome-wide analysis of the U-box E3 ubiquitin ligase enzyme gene family in tomato". Scientific Reports. 10 (9581). doi:10.1038/s41598-020-66553-1. PMID 32533036 Check |pmid= value (help). Retrieved 27 August 2020.

External links