Aspergillosis laboratory findings: Difference between revisions
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__NOTOC__ | __NOTOC__ | ||
{{Aspergillosis}} | {{Aspergillosis}} | ||
{{CMG}}; {{AE}} {{HL}} | {{CMG}}; {{AE}} {{HL}}; {{YD}}; {{SSK}} | ||
==Overview== | ==Overview== | ||
Laboratory findings consistent with the diagnosis of aspergillosis include positive aspergillus antibody test, positive [[galactomannan]] test, | PCR is the method of choice for the diagnosis of aspergillosis. A single negative [[PCR]] result is sufficient to rule out aspergillosis, whereas two positive test results are required to confirm the diagnosis. Laboratory findings consistent with the diagnosis of aspergillosis include positive aspergillus antibody test, positive [[galactomannan]] test, elevated concentration of [[IgE]] [[immunoglobulin]], and positive culture from [[sputum]] or [[bronchoalveolar lavage]].<ref name="wiki">Aspergillosis. Wikipedia (2015) https://en.wikipedia.org/wiki/Aspergillosis#Diagnosis Accessed on February, 8 2016</ref><ref name="pmid21177400">{{cite journal| author=Cuenca-Estrella M, Bassetti M, Lass-Flörl C, Rácil Z, Richardson M, Rogers TR| title=Detection and investigation of invasive mould disease. | journal=J Antimicrob Chemother | year= 2011 | volume= 66 Suppl 1 | issue= | pages= i15-24 | pmid=21177400 | doi=10.1093/jac/dkq438 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=21177400 }} </ref><ref name="pmid18384212">{{cite journal| author=Kradin RL, Mark EJ| title=The pathology of pulmonary disorders due to Aspergillus spp. | journal=Arch Pathol Lab Med | year= 2008 | volume= 132 | issue= 4 | pages= 606-14 | pmid=18384212 | doi=10.1043/1543-2165(2008)132[606:TPOPDD]2.0.CO;2 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=18384212 }} </ref> | ||
==Laboratory Findings== | |||
==Polymerase Chain Reaction== | |||
*PCR is the method of choice for the diagnosis of ''Aspergillosis''. | |||
* A single negative [[PCR]] result is sufficient to rule out a diagnosis of a probable invasive aspergillosis.<ref name="pmid19179225">{{cite journal| author=Mengoli C, Cruciani M, Barnes RA, Loeffler J, Donnelly JP| title=Use of PCR for diagnosis of invasive aspergillosis: systematic review and meta-analysis. | journal=Lancet Infect Dis | year= 2009 | volume= 9 | issue= 2 | pages= 89-96 | pmid=19179225 | doi=10.1016/S1473-3099(09)70019-2 | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=19179225 }} </ref> | |||
* While, two positive test results are required to confirm a diagnosis of aspergillosis. | |||
==Other Laboratory Findings== | |||
====Complete Blood Count==== | ====Complete Blood Count==== | ||
* [[Eosinophilia]] | |||
====Electrophoresis==== | ====Electrophoresis==== | ||
* Elevated concentration of serum [[immunoglobulin]] E ([[IgE]]) | |||
====Biomarkers==== | ====Biomarkers==== | ||
====Culture | * Positive [[galactomannan]] assay | ||
* Positive beta-D-glucan assay | |||
====Culture==== | |||
* Positive culture and staining from [[sputum]] or from [[bronchoalveolar lavage]] (BAL) | |||
* Culture can be performed on a variety of [[sterile]] specimens. [[Aspergillus]] spp. typically appear as rapidly growing [[mold]]s that are visible 1-3 days following incubation. | |||
* Patients with invasive aspergillosis may have negative cultures. | |||
* | |||
==References== | ==References== | ||
{{reflist|2}} | {{reflist|2}} | ||
[[Category:Fungal diseases]] | [[Category:Fungal diseases]] | ||
Latest revision as of 17:01, 18 September 2017
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Haytham Allaham, M.D. [2]; Yazan Daaboul, M.D.; Serge Korjian M.D.
Overview
PCR is the method of choice for the diagnosis of aspergillosis. A single negative PCR result is sufficient to rule out aspergillosis, whereas two positive test results are required to confirm the diagnosis. Laboratory findings consistent with the diagnosis of aspergillosis include positive aspergillus antibody test, positive galactomannan test, elevated concentration of IgE immunoglobulin, and positive culture from sputum or bronchoalveolar lavage.[1][2][3]
Polymerase Chain Reaction
- PCR is the method of choice for the diagnosis of Aspergillosis.
- A single negative PCR result is sufficient to rule out a diagnosis of a probable invasive aspergillosis.[4]
- While, two positive test results are required to confirm a diagnosis of aspergillosis.
Other Laboratory Findings
Complete Blood Count
Electrophoresis
- Elevated concentration of serum immunoglobulin E (IgE)
Biomarkers
- Positive galactomannan assay
- Positive beta-D-glucan assay
Culture
- Positive culture and staining from sputum or from bronchoalveolar lavage (BAL)
- Culture can be performed on a variety of sterile specimens. Aspergillus spp. typically appear as rapidly growing molds that are visible 1-3 days following incubation.
- Patients with invasive aspergillosis may have negative cultures.
References
- ↑ Aspergillosis. Wikipedia (2015) https://en.wikipedia.org/wiki/Aspergillosis#Diagnosis Accessed on February, 8 2016
- ↑ Cuenca-Estrella M, Bassetti M, Lass-Flörl C, Rácil Z, Richardson M, Rogers TR (2011). "Detection and investigation of invasive mould disease". J Antimicrob Chemother. 66 Suppl 1: i15–24. doi:10.1093/jac/dkq438. PMID 21177400.
- ↑ Kradin RL, Mark EJ (2008). "The pathology of pulmonary disorders due to Aspergillus spp". Arch Pathol Lab Med. 132 (4): 606–14. doi:10.1043/1543-2165(2008)132[606:TPOPDD]2.0.CO;2. PMID 18384212.
- ↑ Mengoli C, Cruciani M, Barnes RA, Loeffler J, Donnelly JP (2009). "Use of PCR for diagnosis of invasive aspergillosis: systematic review and meta-analysis". Lancet Infect Dis. 9 (2): 89–96. doi:10.1016/S1473-3099(09)70019-2. PMID 19179225.