Trichomoniasis laboratory findings: Difference between revisions
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== Laboratory Findings == | == Laboratory Findings == | ||
Trichomoniasis is diagnosed by visually observing the [[trichomonads]] via a microscope. In women, the examiner collects the specimen during a pelvic examination by inserting a [[Speculum (medical)|speculum]] into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. | Trichomoniasis is diagnosed by visually observing the [[trichomonads]] via a microscope. In women, the examiner collects the specimen during a pelvic examination by inserting a [[Speculum (medical)|speculum]] into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor [[Sensitivity and specificity|sensitivity]] of the tests; in 2011 a study reported a more reliable test.<ref name=Andrea&Chapin2011>{{cite journal| author=Andrea SB, Chapin KC| title=Comparison of Aptima Trichomonas vaginalis Transcription-Mediated Amplification Assay and BD Affirm VPIII for Detection of T. vaginalis in Symptomatic Women: Performance Parameters and Epidemiological Implications. | journal=J Clin Microbiol | year= 2011 | volume= 49 | issue= 3 | pages= 866–9 | pmid=21248097 | doi=10.1128/JCM.02367-10 | pmc= | url= | laysummary = http://www.eurekalert.org/pub_releases/2011-03/l-amd030811.php}}</ref> | ||
In men, wet preparation is insensitive, and culture testing of urethral swab, urine, and semen is required for optimal sensitivity. No FDA-cleared PCR test for T. vaginalis is available in the United States, but such testing might be available from commercial laboratories that have developed their own PCR tests. [http://www.cdc.gov/std/trichomonas/STDFact-Trichomoniasis.htm][http://www.cdc.gov/std/treatment/2006/vaginal-discharge.htm#vagdis3] | In men, wet preparation is insensitive, and culture testing of urethral swab, urine, and semen is required for optimal sensitivity. No FDA-cleared PCR test for T. vaginalis is available in the United States, but such testing might be available from commercial laboratories that have developed their own PCR tests. [http://www.cdc.gov/std/trichomonas/STDFact-Trichomoniasis.htm][http://www.cdc.gov/std/treatment/2006/vaginal-discharge.htm#vagdis3] | ||
Revision as of 19:29, 20 December 2012
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Overview
Diagnosis of vaginal trichomoniasis is usually performed by microscopy of vaginal secretions, but this method has a sensitivity of only approximately 60%–70% and requires immediate evaluation of wet preparation slide for optimal results.
Laboratory Findings
Trichomoniasis is diagnosed by visually observing the trichomonads via a microscope. In women, the examiner collects the specimen during a pelvic examination by inserting a speculum into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor sensitivity of the tests; in 2011 a study reported a more reliable test.[1]
In men, wet preparation is insensitive, and culture testing of urethral swab, urine, and semen is required for optimal sensitivity. No FDA-cleared PCR test for T. vaginalis is available in the United States, but such testing might be available from commercial laboratories that have developed their own PCR tests. [2][3]
Two trophozoites of Trichomonas vaginalis obtained from in vitro culture. Smear was stained with Giemsa.[4]
References
- ↑ Andrea SB, Chapin KC (2011). "Comparison of Aptima Trichomonas vaginalis Transcription-Mediated Amplification Assay and BD Affirm VPIII for Detection of T. vaginalis in Symptomatic Women: Performance Parameters and Epidemiological Implications". J Clin Microbiol. 49 (3): 866–9. doi:10.1128/JCM.02367-10. PMID 21248097. Lay summary.