Aprotinin

Revision as of 22:25, 8 August 2012 by WikiBot (talk | contribs) (Bot: Automated text replacement (-{{SIB}} + & -{{EH}} + & -{{EJ}} + & -{{Editor Help}} + & -{{Editor Join}} +))
Jump to navigation Jump to search
Aprotinin
Clinical data
Synonymstrasylol, bovine pancreatic trypsin inhibitor
Pregnancy
category
  • X
Dependence
liability		none
Routes of
administration		intravenous
ATC code
Legal status
Legal status
  • RX/POM
Pharmacokinetic data
Bioavailability100% (IV)
Identifiers
CAS Number
PubChem CID
E number{{#property:P628}}
ECHA InfoCard{{#property:P2566}}Lua error in Module:EditAtWikidata at line 36: attempt to index field 'wikibase' (a nil value).
Chemical and physical data
FormulaC284H432N84O79S7
Molar mass6511.51

WikiDoc Resources for Aprotinin

Articles

Most recent articles on Aprotinin

Most cited articles on Aprotinin

Review articles on Aprotinin

Articles on Aprotinin in N Eng J Med, Lancet, BMJ

Media

Powerpoint slides on Aprotinin

Images of Aprotinin

Photos of Aprotinin

Podcasts & MP3s on Aprotinin

Videos on Aprotinin

Evidence Based Medicine

Cochrane Collaboration on Aprotinin

Bandolier on Aprotinin

TRIP on Aprotinin

Clinical Trials

Ongoing Trials on Aprotinin at Clinical Trials.gov

Trial results on Aprotinin

Clinical Trials on Aprotinin at Google

Guidelines / Policies / Govt

US National Guidelines Clearinghouse on Aprotinin

NICE Guidance on Aprotinin

NHS PRODIGY Guidance

FDA on Aprotinin

CDC on Aprotinin

Books

Books on Aprotinin

News

Aprotinin in the news

Be alerted to news on Aprotinin

News trends on Aprotinin

Commentary

Blogs on Aprotinin

Definitions

Definitions of Aprotinin

Patient Resources / Community

Patient resources on Aprotinin

Discussion groups on Aprotinin

Patient Handouts on Aprotinin

Directions to Hospitals Treating Aprotinin

Risk calculators and risk factors for Aprotinin

Healthcare Provider Resources

Symptoms of Aprotinin

Causes & Risk Factors for Aprotinin

Diagnostic studies for Aprotinin

Treatment of Aprotinin

Continuing Medical Education (CME)

CME Programs on Aprotinin

International

Aprotinin en Espanol

Aprotinin en Francais

Business

Aprotinin in the Marketplace

Patents on Aprotinin

Experimental / Informatics

List of terms related to Aprotinin

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]


Aprotinin, also known as bovine pancreatic trypsin inhibitor, BPTI (Trasylol®, Bayer) is a protein, that is used as medication administered by injection to reduce bleeding during complex surgery, such as heart and liver surgery. Its main effect is the slowing down of fibrinolysis, the process that leads to the breakdown of blood clots. The aim in its use is to decrease the need for blood transfusions during surgery, as well as end-organ damage due to hypotension (low blood pressure) as a result of marked blood loss.

Chemistry

Aprotinin is a monomeric (single-chain) globular polypeptide derived from bovine lung tissue; it has a molecular weight of 6512 and consists of 16 different amino acids arranged in a chain of 58 amino acid residues.[1][2].

Its amino acid sequence is RPDFC LEPPY TGPCK ARIIR YFYNA KAGLC QTFVY GGCRA KRNNF KSAED CMRTC GGA.[3]

The stability of the molecule is due to the 3 disulfide bonds linking the 6 cysteine members of the chain (Cys5-Cys55, Cys14-Cys38 and Cys30-Cys51).[4] The lysine (15)-alanine (16) sequence on this strongly basic polypeptide represents the active centre.

Aprotinin is the axiomic member of the protein family of Kunitz-type serine protease inhibitors.

It is one of the most thoroughly studied proteins in terms of structure and folding pathway. BPTI was one of the first proteins to have its structure solved by NMR spectroscopy. Nevertheless, its physiological function remains unknown.

Mechanism of action

Aprotinin inhibits several serine proteases, specifically trypsin, chymotrypsin and plasmin at a concentration of about 125,000 IU/ml, and kallikrein at 300,000 IU/ml[2]. Its action on kallikrein leads to the inhibition of the formation of factor XIIa. As a result, both the intrinsic pathway of coagulation and fibrinolysis are inhibited. Its action on plasmin independently slows fibrinolysis[1].

Efficacy

In cardiac surgery with a high risk of significant blood loss, aprotinin significantly reduced bleeding, mortality and hospital stay[2]. Beneficial effects were also reported in high-risk orthopedic surgery[2]. In liver transplantation, initial reports of benefit were overshadowed by concerns about toxicity[5].

In a meta-analysis performed in 2004, transfusion requirements decreased by 39% in coronary artery bypass graft (CABG) surgery[6]. In orthopedic surgery, a decrease of blood transfusions was likewise confirmed[7].

Safety

There have been concerns about the safety of aprotinin[2]. Anaphylaxis (a severe allergic reaction) occurs at a rate of 1:200 in first-time use, but serology (measuring antibodies against aprotinin in the blood) is not carried out in practice to predict anaphylaxis risk because the correct interpretation of these tests is difficult.[2]

Thrombosis, presumably from overactive inhibition of the fibrinolytic system, may occur at a higher rate, but until 2006 there was limited evidence for this association[2][6]. Similarly, while biochemical measures of renal function were known to occasionally deteriorate, there was no evidence that this greatly influenced outcomes[2]. A study performed in cardiac surgery patients reported in 2006 showed that there was indeed a risk of acute renal failure, myocardial infarction and heart failure, as well as stroke and encephalopathy[8] The study authors recommend older antifibrinolytics (such as tranexamic acid) in which these risks were not documented[8]. The same group updated their data in 2007 and demonstrated similar findings.[9]

In September 2006, Bayer A.G. was faulted by the FDA for not revealing during testimony the existence of a commissioned retrospective study of 67,000 patients, 30,000 of whom received Trasylol and the rest other anti-fibrinolytics. The study concluded Trasylol carried greater risks. The FDA was alerted to the study by one of the researchers involved. Although the FDA issued a statement of concern they did not change their recommendation that the drug may benefit certain subpopulations of patients.[10] In a Public Health Advisory Update dated October 3, 2006, the FDA recommended that "physicians consider limiting Trasylol use to those situations in which the clinical benefit of reduced blood loss is necessary to medical management and outweighs the potential risks" and carefully monitor patients. [11]

On October 29, 2006 the Food and Drug Administration issued a warning that aprotinin may have serious kidney and cardiovascular toxicity. The producer, Bayer, reported to the FDA that additional observation studies showed that it may increase the chance for death, serious kidney damage, congestive heart failure and strokes. FDA warned clinicians to consider limiting use to those situations where the clinical benefit of reduced blood loss is essential to medical management and outweighs the potential risks.[12]

No cases of bovine spongiform encephalopathy transmission by aprotinin have been reported, although the drug was withdrawn in Italy due to fears of this [2].

In vitro use

Small amounts of aprotinin can be added to tubes of drawn blood to enable laboratory measurement of certain rapidly degraded proteins such as glucagon. In cell biology aprotinin is used as a enzyme inhibitor to prevent protein degradation during lysis or homogenizaton of cells and tissues.

History

Initially named "kallikrein inactivator", aprotinin was first isolated from cow parotid glands in 1928[13] and independently as "bovine pancreatic trypsin inhibitor" from cow pancreas in 1936.[14] It was purified from bovine lung in 1964.[15] As it inhibits pancreatic enzymes, it was initially used in the treatment for acute pancreatitis, in which destruction of the gland by its own enzymes is thought to be part of the pathogenesis.[16] Its use in major surgery commenced in the 1960s.[17]

References

  1. ↑ 1.0 1.1 Mannucci PM. Hemostatic drugs. N Engl J Med 1998;339:245-53. PMID 9673304.
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Mahdy AM, Webster NR. Perioperative systemic haemostatic agents. Br J Anaesth 2004;93:842-58. PMID 15277296.
  3. ↑ Kassell B, Radicevic M, Ansfield MJ, Laskowski Sr M. The basic trypsin inhibitor of bovine pancreas IV. The linear sequence of the 58 amino acids. Biochem Biophys Res Commun 1965;18:255-8. PMID 14282026.
  4. ↑ Kassell B, Laskowski M Sr. The basic trypsin inhibitor of bovine pancreas. V. The disulfide linkages. Biochem Biophys Res Commun 1965;20:463-8. PMID 5860161.
  5. ↑ Xia VW, Steadman RH. Antifibrinolytics in orthotopic liver transplantation: current status and controversies. Liver Transpl 2005;11:10-8. PMID 15690531.
  6. ↑ 6.0 6.1 Sedrakyan A, Treasure T, Elefteriades JA. Effect of aprotinin on clinical outcomes in coronary artery bypass graft surgery: a systematic review and meta-analysis of randomized clinical trials. J Thorac Cardiovasc Surg 2004;128:442-8. PMID 15354106.
  7. ↑ Shiga T, Wajima Z, Inoue T, Sakamoto A. Aprotinin in major orthopedic surgery: a systematic review of randomized controlled trials. Anesth Analg 2005;101:1602-7. PMID 16301226.
  8. ↑ 8.0 8.1 Mangano DT, Tudor IC, Dietzel C; Multicenter Study of Perioperative Ischemia Research Group; Ischemia Research and Education Foundation. The risk associated with aprotinin in cardiac surgery. N Engl J Med 2006;354:353-65. PMID 16436767.
  9. ↑ Mangano D, Miao Y, Vuylsteke A, Tudor I, Juneja R, Filipescu D, Hoeft A, Fontes M, Hillel Z, Ott E, Titov T, Dietzel C, Levin J (2007). "Mortality associated with aprotinin during 5 years following coronary artery bypass graft surgery". JAMA. 297 (5): 471–9. PMID 17284697.
  10. ↑ Gardiner Harris. F.D.A. says Bayer Failed to Reveal Drug Risk Study. New York Times; September 30, 2006
  11. ↑ Trasylol Public Health Advisory Update; October 3, 2006
  12. ↑ U.S. Food and Drug Administration. "Information for Healthcare Professionals; Aprotinin (marketed as Trasylol)". Retrieved 2006-10-30.
  13. ↑ Kraut H, Frey EK, Bauer E. Z Physiol Chem 1928;175:97-114.
  14. ↑ Kunitz M, Northrup J. Isolation from beef pancreas of crystalline trypsinogen, trypsin, trypsin inhibitor, and an inhibitor trypsin compound. J Gen Physiol 1936;19:991-1007. PDF.
  15. ↑ Kraut H, Bhargava N. Versuche zur Isolierung des Kallikrein-Inaktivators aus Rinderlunge and seine Identifizierung mit dem Inaktivator aus Rinderparotis. Z Physiol Chem 1964;328:231-7. PMID 14330402.
  16. ↑ Nugent FW, Warren KW, Jonasson H, Garciadeparedes G. Early experience with trasylol in the treatment of acute pancreatitis. South Med J 1964;57:1317-21. PMID 14195953.
  17. ↑ Tice DA, Worth Jr MH, Clauss RH, Reed GH. The inhibition of trasylol of fibrinolytic activity associated with cardiovascular operations. Surg Gynecol Obstet 1964;119:71-4. PMID 14179354.

External links

de:Aprotinin


Template:WikiDoc Sources

Retrieved from "https://www.wikidoc.org/index.php?title=Aprotinin&oldid=668937"