Centromere protein B

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External IDsGeneCards: [1]
RefSeq (mRNA)



RefSeq (protein)



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Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. In humans, centromere protein B is encoded by the CENPB gene.[1][2][3]


Centromere protein B is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Clinical significance

Centromere protein B is a potential biomarker of small-cell lung cancer.[4]

See also


  1. "Entrez Gene: centromere protein B".
  2. Sugimoto K, Yata H, Himeno M (July 1993). "Mapping of the human CENP-B gene to chromosome 20 and the CENP-C gene to chromosome 12 by a rapid cycle DNA amplification procedure". Genomics. 17 (1): 240–2. doi:10.1006/geno.1993.1312. PMID 8406460.
  3. Ando S, Yang H, Nozaki N, Okazaki T, Yoda K (April 2002). "CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells". Molecular and Cellular Biology. 22 (7): 2229–41. doi:10.1128/MCB.22.7.2229-2241.2002. PMC 133672. PMID 11884609.
  4. Briasoulis E, Kamposioras K, Tzovaras V, Pafitanis G, Kostoula A, Mavridis A, Pavlidis N (May 2008). "CENP-B specific anti-centromere autoantibodies heralding small-cell lung cancer. A case study and review of the literature". Lung Cancer. 60 (2): 302–6. doi:10.1016/j.lungcan.2007.09.014. PMID 17980453.

Further reading

  • Saxena A, Wong LH, Kalitsis P, Earle E, Shaffer LG, Choo KH (September 2002). "Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc". Human Molecular Genetics. 11 (19): 2319–29. doi:10.1093/hmg/11.19.2319. PMID 12217960.
  • Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (April 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
  • Orthaus S, Biskup C, Hoffmann B, Hoischen C, Ohndorf S, Benndorf K, Diekmann S (January 2008). "Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells". Chembiochem. 9 (1): 77–92. doi:10.1002/cbic.200700358. PMID 18072184.
  • Tawaramoto MS, Park SY, Tanaka Y, Nureki O, Kurumizaka H, Yokoyama S (December 2003). "Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution". The Journal of Biological Chemistry. 278 (51): 51454–61. doi:10.1074/jbc.M310388200. PMID 14522975.
  • Tanaka Y, Tachiwana H, Yoda K, Masumoto H, Okazaki T, Kurumizaka H, Yokoyama S (December 2005). "Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences". The Journal of Biological Chemistry. 280 (50): 41609–18. doi:10.1074/jbc.M509666200. PMID 16183641.
  • "Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls". Nature. 447 (7145): 661–78. June 2007. doi:10.1038/nature05911. PMC 2719288. PMID 17554300.
  • Tanaka Y, Kurumizaka H, Yokoyama S (January 2005). "CpG methylation of the CENP-B box reduces human CENP-B binding". The FEBS Journal. 272 (1): 282–9. doi:10.1111/j.1432-1033.2004.04406.x. PMID 15634350.
  • Sowa ME, Bennett EJ, Gygi SP, Harper JW (July 2009). "Defining the human deubiquitinating enzyme interaction landscape". Cell. 138 (2): 389–403. doi:10.1016/j.cell.2009.04.042. PMC 2716422. PMID 19615732.
  • Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabási AL, Vidal M, Zoghbi HY (May 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.
  • Lomonte P, Morency E (February 2007). "Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0". FEBS Letters. 581 (4): 658–62. doi:10.1016/j.febslet.2007.01.027. PMID 17258208.
  • Suzuki N, Nakano M, Nozaki N, Egashira S, Okazaki T, Masumoto H (February 2004). "CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization". The Journal of Biological Chemistry. 279 (7): 5934–46. doi:10.1074/jbc.M306477200. PMID 14612452.
  • Hellwig D, Münch S, Orthaus S, Hoischen C, Hemmerich P, Diekmann S (August 2008). "Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B". Journal of Biophotonics. 1 (3): 245–54. doi:10.1002/jbio.200810014. PMID 19412974.
  • Robitaille G, Hénault J, Christin MS, Senécal JL, Raymond Y (November 2007). "The nuclear autoantigen CENP-B displays cytokine-like activities toward vascular smooth muscle cells". Arthritis and Rheumatism. 56 (11): 3814–26. doi:10.1002/art.22972. PMID 17968937.
  • Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K (February 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase". Genes to Cells. 9 (2): 105–20. doi:10.1111/j.1365-2443.2004.00705.x. PMID 15009096.
  • Perdoni F, Bottone MG, Soldani C, Veneroni P, Alpini C, Pellicciari C, Scovassi AI (August 2009). "Distribution of centromeric proteins and PARP-1 during mitosis and apoptosis". Annals of the New York Academy of Sciences. 1171: 32–7. doi:10.1111/j.1749-6632.2009.04729.x. PMID 19723035.
  • Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (January 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
  • Robitaille G, Christin MS, Clément I, Senécal JL, Raymond Y (September 2009). "Nuclear autoantigen CENP-B transactivation of the epidermal growth factor receptor via chemokine receptor 3 in vascular smooth muscle cells". Arthritis and Rheumatism. 60 (9): 2805–16. doi:10.1002/art.24765. PMID 19714638.

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.