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<!-- The PBB_Controls template provides controls for Protein Box Bot, please see Template:PBB_Controls for details. -->
{{Infobox_gene}}
{{PBB_Controls
'''Mannose-binding lectin''' ('''MBL'''), also called '''mannan-binding lectin''' or '''mannan-binding protein''' ('''MBP'''), is a [[lectin]] that is instrumental in [[innate immunity]]<ref>{{cite journal  |vauthors=Fraser IP, Koziel H, Ezekowitz RA |title=The serum mannose-binding protein and the macrophage mannose receptor are pattern recognition molecules that link innate and adaptive immunity. |journal=Semin. Immunol. |volume=10 |issue= 5 |pages= 363–72 |year= 1998 |pmid= 9799711 |doi= 10.1006/smim.1998.0141 }}</ref><ref>{{cite journal  |vauthors=Worthley DL, Bardy PG, Mullighan CG |title=Mannose-binding lectin: biology and clinical implications. |journal=Internal medicine journal |volume=35 |issue= 9 |pages= 548–55 |year= 2005 |pmid= 16105157 |doi= 10.1111/j.1445-5994.2005.00908.x }}</ref> as an opsonin and via the [[lectin pathway]].
| update_page = yes
| require_manual_inspection = no
| update_protein_box = yes
| update_summary = no
| update_citations = yes
}}


<!-- The GNF_Protein_box is automatically maintained by Protein Box Bot. See Template:PBB_Controls to Stop updates. -->
==Structure==
{{GNF_Protein_box
MBL has an oligomeric structure (400-700 kDa), built of subunits that contain three presumably identical peptide chains of about 30 kDa each.
| image = PBB_Protein_MBL2_image.jpg
 
| image_source = [[Protein_Data_Bank|PDB]] rendering based on 1hup.
Although MBL can form several oligomeric forms, there are indications that dimers and trimers are biologically active as an opsonin and at least a tetramer form is needed for activation of complement.<ref name="pmid7634089">{{cite journal |vauthors=Sheriff S, Chang CY, Ezekowitz RA | title = Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple alpha-helical coiled-coil | journal = Nat. Struct. Biol. | volume = 1 | issue = 11 | pages = 789–94 |date=November 1994 | pmid = 7634089 | doi = 10.1038/nsb1194-789| url = | issn = }}</ref>
| PDB = {{PDB2|1hup}}
 
| Name = Mannose-binding lectin (protein C) 2, soluble (opsonic defect)
===Genes and polymorphisms===
| HGNCid = 6922
Human MBL2 gene is located on chromosome 10q11.2-q21.<ref name="pmid2477486">{{cite journal |vauthors=Sastry K, Herman GA, Day L, Deignan E, Bruns G, Morton CC, Ezekowitz RA | title = The human mannose-binding protein gene. Exon structure reveals its evolutionary relationship to a human pulmonary surfactant gene and localization to chromosome 10 | journal = J. Exp. Med. | volume = 170 | issue = 4 | pages = 1175–89 |date=October 1989 | pmid = 2477486 | pmc = 2189467 | doi = 10.1084/jem.170.4.1175}}</ref> Mice have two homologous genes, but in human the first of them was lost. A low level expression of an MBL1 pseudogene 1 (MBL1P1) was detected in liver. The pseudogene encodes a truncated 51-amino acid protein that is homologous to the MBLA isoform in rodents and some primates.<ref name="pmid9501312">{{cite journal |vauthors=Guo N, Mogues T, Weremowicz S, Morton CC, Sastry KN | title = The human ortholog of rhesus mannose-binding protein-A gene is an expressed pseudogene that localizes to chromosome 10 | journal = Mamm. Genome | volume = 9 | issue = 3 | pages = 246–9 |date=March 1998 | pmid = 9501312 | doi = 10.1007/s003359900735}}</ref>
| Symbol = MBL2
 
| AltSymbols =; COLEC1; HSMBPC; MBL; MBP; MBP1; MGC116832; MGC116833
Structural mutations in exon 1 of the human MBL2 gene, at codon 52 (Arg to Cys, allele D), codon 54 (Gly to Asp, allele B) and codon 57 (Gly to Glu, allele C), also independently reduce the level of functional serum MBL by disrupting the collagenous structure of the protein.<ref name="Seyfarth">{{cite journal |vauthors=Seyfarth J, Garred P, Madsen HO | title = The ‘involution’ of mannose-binding lectin | journal = Human Molecular Genetics | volume = 14 | pages = 2859–69 | year = 2005 | pmid = 16115813 | doi = 10.1093/hmg/ddi318 | issue=19}}</ref> Furthermore, several nucleotide substitutions in the promoter region of the MBL2 gene at position −550 (H/L polymorphism), −221 (X/Y polymorphism) and −427, −349, −336, del (−324 to −329), −70 and +4 (P/Q polymorphisms) affect the MBL serum concentration. Both the frequency of structural mutations and the promoter polymorphisms that are in strong linkage disequilibrium vary among ethnic groups resulting in seven major haplotypes: HYPA, LYQA, LYPA, LXPA, LYPB, LYQC and HYPD. Differences in the distribution of these haplotypes are the major cause of interracial variations in MBL serum levels. Both HYPA and LYQA are high-producing haplotypes, LYPA intermediate-producing haplotype and LXPA low-producing haplotype, whereas LYPB, LYQC and HYPD are defective haplotypes, which cause a severe MBL deficiency.<ref name="OMIM_614372">{{OMIM|614372|mannose-binding protein deficiency}}</ref>
| OMIM = 154545
| ECnumber =
| Homologene = 88328
| MGIid = 96924
| GeneAtlas_image1 = PBB_GE_MBL2_207256_at_tn.png
| Function = {{GNF_GO|id=GO:0005102 |text = receptor binding}} {{GNF_GO|id=GO:0005509 |text = calcium ion binding}} {{GNF_GO|id=GO:0005529 |text = sugar binding}} {{GNF_GO|id=GO:0005537 |text = mannose binding}}
| Component = {{GNF_GO|id=GO:0005615 |text = extracellular space}} {{GNF_GO|id=GO:0005737 |text = cytoplasm}} {{GNF_GO|id=GO:0016020 |text = membrane}}
| Process = {{GNF_GO|id=GO:0001867 |text = complement activation, lectin pathway}} {{GNF_GO|id=GO:0006817 |text = phosphate transport}} {{GNF_GO|id=GO:0006958 |text = complement activation, classical pathway}} {{GNF_GO|id=GO:0006979 |text = response to oxidative stress}}
| Orthologs = {{GNF_Ortholog_box
    | Hs_EntrezGene = 4153
    | Hs_Ensembl = ENSG00000165471
    | Hs_RefseqProtein = NP_000233
    | Hs_RefseqmRNA = NM_000242
    | Hs_GenLoc_db =
    | Hs_GenLoc_chr = 10
    | Hs_GenLoc_start = 54195146
    | Hs_GenLoc_end = 54201466
    | Hs_Uniprot = P11226
    | Mm_EntrezGene = 17195
    | Mm_Ensembl = ENSMUSG00000024863
    | Mm_RefseqmRNA = NM_010776
    | Mm_RefseqProtein = NP_034906
    | Mm_GenLoc_db =
    | Mm_GenLoc_chr = 19
    | Mm_GenLoc_start = 30298939
    | Mm_GenLoc_end = 30305678
    | Mm_Uniprot = Q3UEK1
  }}
}}
'''Mannose binding lectin''' (MBL), also named [[mannose]]- or [[mannan]]-binding protein (MBP), is an important factor in [[innate immunity]].  


==Function==
Both MBL2 and MBL1P1 genes has been repeatedly hit throughout evolution of primates. The latter silenced eventually by mutations in the glycine residues of the collagen-like region. It has been selectively turned off during evolution through the same molecular mechanisms causing the MBL2 variant alleles in man, suggesting an evolutionary selection for low-producing MBL genes.<ref name="Seyfarth" />
MBL belongs to the class of collectins in the [[C-type]] [[lectin]] superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host.  


MBL recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including [[bacteria]], [[viruses]], [[protozoa]] and [[fungi]].  
===Posttranslational modifications===
In rat [[hepatocyte]]s, MBL is synthesized in the [[rough endoplasmic reticulum]]. While in [[Golgi apparatus|Golgi]], it undergoes two distinct [[posttranslational modification]]s and is assembled into high molecular weight multimeric complexes. The modifications produce MBL in multiple forms of slightly various molecular masses and pI from 5.7 to 6.2.<ref name="Colley">{{cite journal |vauthors=Colley KJ, Baenziger JU | title = Identification of the posttranslational modifications of the core-specific lectin. The core-specific lectin contains hydroxyproline, hydroxylysine, and glucosylgalactosylhydroxylysine residues | journal = J Biol Chem | volume = 262 | pages = 10290–5 | year = 1987 | pmid = 3611062 | doi = | url = | issn = | issue=21}}</ref> Proteolytic cleavage also resulted in removal of the 20-aa N-terminal signal peptide,<ref>{{cite web |url=https://www.ncbi.nlm.nih.gov/protein/NP_000233.1 |title=Mannose-binding protein C precursor [Homo sapiens] |format= |work= |accessdate=2012-01-03}}</ref> and hydroxylation and glycosylation were also detected.<ref name="Colley" /> Some cysteine residues can be converted to dehydroalanin.<ref>{{cite journal |vauthors=Jensen PH, Laursen I, Matthiesen F, Højrup P | title = Posttranslational modifications in human plasma MBL and human recombinant MBL | journal =  Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics  | volume = 1774 | pages = 335–44. | year = 2007 | pmid =  | doi = 10.1016/j.bbapap.2006.12.008 | url = | issn = }}</ref>


Binding of MBL to a [[micro-organism]] results in activation of the [[lectin pathway]] of the [[complement system]].
==Function==
MBL belongs to the class of [[collectin]]s in the [[C-type lectin|C-type]] [[lectin]] superfamily, whose function appears to be [[Pattern recognition receptor|pattern recognition]] in the first line of defense in the pre-immune host. MBL recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including [[bacteria]], [[viruses]], [[protozoa]] and [[fungi]]. Binding of MBL to a [[micro-organism]] results in activation of the [[lectin pathway]] of the [[complement system]].


==Structure==
Another important function of MBL is that this molecule binds [[Senescence|senescent]]<ref>{{cite journal |vauthors=Tomaiuolo R, Ruocco A, Salapete C, Carru C, Baggio G, Franceschi C, Zinellu A, Vaupel J, Bellia C, Lo Sasso B, Ciaccio M, Castaldo G, Deiana L | title = Activity of mannose-binding lectin (MBL) in centenarians | journal = Aging Cell | volume = 11| issue = 3| pages = 394–400|date=March 2012 | pmid = 22239660 | doi = 10.1111/j.1474-9726.2012.00793.x }}</ref> and [[Apoptosis|apoptotic]] cells and enhances [[phagocytosis|engulfment]] of whole, intact apoptotic cells, as well as cell debris by [[phagocyte]]s.<ref name="pmid11560994">{{cite journal |vauthors=Ogden CA, deCathelineau A, Hoffmann PR, Bratton D, Ghebrehiwet B, Fadok VA, Henson PM | title = C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells | journal = J. Exp. Med. | volume = 194 | issue = 6 | pages = 781–95 |date=September 2001 | pmid = 11560994 | pmc = 2195958 | doi = 10.1084/jem.194.6.781 }}</ref><ref name="pmid15749852">{{cite journal |vauthors=Stuart LM, Takahashi K, Shi L, Savill J, Ezekowitz RA | title = Mannose-binding lectin-deficient mice display defective apoptotic cell clearance but no autoimmune phenotype | journal = J. Immunol. | volume = 174 | issue = 6 | pages = 3220–6 |date=March 2005 | pmid = 15749852 | doi = 10.4049/jimmunol.174.6.3220}}</ref>
MBL has an oligomeric structure (400-700 kDa), built of subunits that contain three identical peptide chains of 32 kDa each.  


Although MBL can form several oligomeric forms, there are indications that dimers and trimers are not biologically active and at least a tetramer form is needed for activation of complement.
===Activation===
The complement system can be activated through three pathways: [[Classical complement pathway|the classical pathway]], [[Alternative complement pathway|the alternative pathway]], and the [[lectin pathway]]. One way that the most-recently discovered lectin pathway is activated is through mannose-binding lectin protein. MBL binds to carbohydrates (to be specific, D-mannose and L-fucose residues) found on the surfaces of many pathogens.


==Activation==
For example, MBL has been shown to bind to:
The complement system can be activated through three pathways [[Classical complement pathway|the classical pathway]], [[Alternative complement pathway|the alternative pathway]], and the mannose-binding (MB) [[Mannan-binding lectin pathway|lectin pathway]]. The most-recently discovered mannose-binding lectin pathway activates complement through the mannose-binding lectin protein. MBL binds to carbohydrates (specifically Mannose and Fucose residues) found on the surface of many pathogens.
* [[yeast]]s such as [[Candida albicans]]<ref>{{cite journal |last1=Choteau |first1=L |last2=Parny |first2=M |last3=François |first3=N |last4=Bertin |first4=B |last5=Fumery |first5=M |last6=Dubuquoy |first6=L |last7=Takahashi |first7=K |last8=Colombel |first8=J-F |last9=Jouault |first9=T |last10=Poulain |first10=D |last11=Sendid |first11=B |last12=Jawhara |first12=S |title=Role of mannose-binding lectin in intestinal homeostasis and fungal elimination |journal=Mucosal Immunology |date=7 October 2015 |volume=9 |issue=3 |pages=767–776 |doi=10.1038/mi.2015.100 |url=https://www.nature.com/articles/mi2015100 |language=En |issn=1933-0219}}</ref><ref name="pmid20097424">{{cite journal |vauthors=de Jong MA, Vriend LE, Theelen B, Taylor ME, Fluitsma D, Boekhout T, Geijtenbeek TB | title = C-type lectin Langerin is a beta-glucan receptor on human Langerhans cells that recognizes opportunistic and pathogenic fungi | journal = Mol. Immunol. | volume = 47 | issue = 6 | pages = 1216–25 |date=March 2010 | pmid = 20097424 | pmc = 2837148 | doi = 10.1016/j.molimm.2009.12.016 }}</ref>
* [[viruses]] such as [[HIV]]<ref name="pmid15488604">{{cite journal |vauthors=Ji X, Gewurz H, Spear GT | title = Mannose binding lectin (MBL) and HIV | journal = Mol. Immunol. | volume = 42 | issue = 2 | pages = 145–52 |date=February 2005 | pmid = 15488604 | doi = 10.1016/j.molimm.2004.06.015 }}</ref> and [[influenza A]]
* many [[bacteria]], including [[Salmonella]]  and [[Streptococci]]
* [[parasite]]s like [[Leishmania]]


For example, MBL has been show to bind to:
===Complexes===
* [[yeast]]s such as [[Candida albicans]]
MBL in the blood is complexed with (bound to) another protein, a serine protease called MASP (MBL-associated serine protease). There are three MASPs: MASP-1, MASP-2 and MASP-3, which have protease domains. There are also sMAP (also called MAp19) and MAp44, which do not have protease domains and are thought to be regulatory molecules of MASPs. MASPs also form complexes with ficolins, which are similar to MBL functionally and structurally with the exception that ficolins recognize their targets through fibrinogen-like domains, unlike MBL.
* [[viruses]] such as [[HIV]] and [[influenza A]]
* many [[bacteria]] including [[Salmonella]] and [[Streptococci]]
* [[parasite]]s like Leishmania


==Complexes==
In order to activate the complement system when MBL binds to its target (for example, mannose on the surface of a bacterium), the MASP protein functions to cleave the blood protein [[Complement component 4|C4]] into C4a and C4b. The C4b fragments can then bind to the surface of the bacterium, and initiate the formation of a [[C3-convertase]].
MBL in the blood is complexed with (bound to) another protein, a [[serine protease]] called [[MASP-2 (protein)|MASP-2]] (MBL-associated serine protease).  


In order to activate the complement system when MBL binds to its target (for example, mannose on the surface of a bacterium), the MASP protein functions to cleave the blood protein [[Complement component 4|C4]] into C4a and C4b. The C4b fragments can then bind to the surface of the bacterium, and initiate the formation of a [[C3 convertase]].  
The subsequent [[complement cascade]] catalyzed by C3-convertase results in creating a [[membrane attack complex]], which causes lysis of the pathogen as well as altered-self in the context of apoptotic and necrotic cells.


The subsequent [[complement cascade]] catalyzed by C3 convertase results in creating a [[membrane attack complex]], which causes lysis of the pathogen that MBL bound to.
MBL/MASP-1 complex also has thrombin-like activity (thrombin clots fibrin to initiate blood clots).  Mice that genetically lack MBL or MASP-1/3 (but not MASP-2/sMAP) have prolonged bleeding time in experimental injury models, although mice are seen to be normal if there is no insult to the body.


==Clinical significance==
==Clinical significance==
It is produced in the [[liver]] as a response to infection, and is part of many other factors termed [[acute phase protein]]s.
It is produced in the [[liver]] as a response to infection, and is part of many other factors termed [[acute phase protein]]s.<ref>{{cite journal | last1 = Herpers | first1 = B L | last2 = Endeman | first2 = H | last3 = de Jong | first3 = B A W | last4 = de Jongh | first4 = B M | last5 = Grutters | first5 = J C | last6 = Biesma | first6 = D H | last7 = vam Velzen-Blad | first7 = H | date = Jun 2009 | title = Acute-phase responsiveness of mannose-binding lectin in community-acquired pneumonia is highly dependent upon MBL2 genotypes | url = | journal = Clin Exp Immunol | volume = 156 | issue = 3| pages = 488–94 | doi = 10.1111/j.1365-2249.2009.03929.x | pmid = 19438602 | pmc=2691978}}</ref> Expression and function in other organs were also suggested.<ref name="pmid17072973">{{cite journal |vauthors=Worthley DL, Bardy PG, Gordon DL, Mullighan CG | title = Mannose-binding lectin and maladies of the bowel and liver | journal = World J. Gastroenterol. | volume = 12 | issue = 40 | pages = 6420–8 |date=October 2006 | pmid = 17072973 | doi = | url = | issn = }}</ref>
The three structural polymorphisms of exon 1 have been reported to cause susceptibility to various common infections, including [[meningococcal disease]].<ref name=HibberdML1999>{{Cite journal | last1 = Hibberd | first1 = M. L. | last2 = Sumiya | first2 = M. | last3 = Summerfield | first3 = J. A. | last4 = Booy | first4 = R. | last5 = Levin | first5 = M. | title = Association of variants of the gene for mannose-binding lectin with susceptibility to meningococcal disease | doi = 10.1016/S0140-6736(98)08350-0 | journal = The Lancet | volume = 353 | issue = 9158 | pages = 1049 | year = 1999 | pmid =  | pmc = }}</ref><ref name=FaberK2007>{{Cite journal | last1 = Faber | first1 = J. | last2 = Schuessler | first2 = T. | last3 = Finn | first3 = A. | last4 = Murdoch | first4 = C. | last5 = Zenz | first5 = W. | last6 = Habermehl | first6 = P. | last7 = Meyer | first7 = C. U. | last8 = Zabel | first8 = B. U. | last9 = Schmitt | first9 = H. J. | last10 = Zepp | doi = 10.1097/01.inf.0000256751.76218.7c | first10 = F. | last11 = Knuf | first11 = M. | title = Age-Dependent Association of Human Mannose-Binding Lectin Mutations with Susceptibility to Invasive Meningococcal Disease in Childhood | journal = The Pediatric Infectious Disease Journal | volume = 26 | issue = 3 | pages = 243–246 | year = 2007 | pmid =  17484222| pmc = }}</ref> However, evidence has been presented that suggests no harmful effect of these variants with regard to mengingococcal disease.<ref name=BradleyDT2012>{{Cite journal | last1 = Bradley | first1 = D. T. | last2 = Bourke | first2 = T. W. | last3 = Fairley | first3 = D. J. | last4 = Borrow | first4 = R. | last5 = Shields | first5 = M. D. | last6 = Young | first6 = I. S. | last7 = Zipfel | first7 = P. F. | last8 = Hughes | first8 = A. E. | title = Genetic susceptibility to invasive meningococcal disease: MBL2 structural polymorphisms revisited in a large case-control study and a systematic review | doi = 10.1111/j.1744-313X.2012.01095.x | journal = International Journal of Immunogenetics | pages = no | year = 2012 | pmid =  | pmc = }}</ref>


==External links==
==External links==
Line 83: Line 48:


==References==
==References==
{{reflist|2}}
{{reflist|colwidth=35em}}
 
==Further reading==
{{refbegin | 2}}
*Sheriff, S.,  Chang, C.Y.,  Ezekowitz, R.A.  (1994) Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple alpha-helical coiled-coil.  Nat.Struct.Biol.  1: 789-794
{{PBB_Further_reading
| citations =
*{{cite journal  | author=Fraser IP, Koziel H, Ezekowitz RA |title=The serum mannose-binding protein and the macrophage mannose receptor are pattern recognition molecules that link innate and adaptive immunity. |journal=Semin. Immunol. |volume=10 |issue= 5 |pages= 363-72 |year= 1998 |pmid= 9799711 |doi= 10.1006/smim.1998.0141 }}
*{{cite journal  | author=Ji X, Gewurz H, Spear GT |title=Mannose binding lectin (MBL) and HIV. |journal=Mol. Immunol. |volume=42 |issue= 2 |pages= 145-52 |year= 2005 |pmid= 15488604 |doi= 10.1016/j.molimm.2004.06.015 }}
*{{cite journal  | author=Worthley DL, Bardy PG, Mullighan CG |title=Mannose-binding lectin: biology and clinical implications. |journal=Internal medicine journal |volume=35 |issue= 9 |pages= 548-55 |year= 2005 |pmid= 16105157 |doi= 10.1111/j.1445-5994.2005.00908.x }}
*{{cite journal  | author=Worthley DL, Bardy PG, Gordon DL, Mullighan CG |title=Mannose-binding lectin and maladies of the bowel and liver. |journal=World J. Gastroenterol. |volume=12 |issue= 40 |pages= 6420-8 |year= 2007 |pmid= 17072973 |doi=  }}
}}
{{refend}}
 


{{PDB Gallery|geneid=4153}}
{{Lectins}}
{{Complement system}}


{{Complement system}}
[[Category:Immune system]]
[[Category:Immune system]]
 
[[Category:Collectins]]
[[pl:Białko wiążące mannozę]]
[[Category:Blood proteins]]
{{WikiDoc Sources}}
[[Category:Human proteins]]
[[Category:Lectins]]

Latest revision as of 20:25, 13 January 2019

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Mannose-binding lectin (MBL), also called mannan-binding lectin or mannan-binding protein (MBP), is a lectin that is instrumental in innate immunity[1][2] as an opsonin and via the lectin pathway.

Structure

MBL has an oligomeric structure (400-700 kDa), built of subunits that contain three presumably identical peptide chains of about 30 kDa each.

Although MBL can form several oligomeric forms, there are indications that dimers and trimers are biologically active as an opsonin and at least a tetramer form is needed for activation of complement.[3]

Genes and polymorphisms

Human MBL2 gene is located on chromosome 10q11.2-q21.[4] Mice have two homologous genes, but in human the first of them was lost. A low level expression of an MBL1 pseudogene 1 (MBL1P1) was detected in liver. The pseudogene encodes a truncated 51-amino acid protein that is homologous to the MBLA isoform in rodents and some primates.[5]

Structural mutations in exon 1 of the human MBL2 gene, at codon 52 (Arg to Cys, allele D), codon 54 (Gly to Asp, allele B) and codon 57 (Gly to Glu, allele C), also independently reduce the level of functional serum MBL by disrupting the collagenous structure of the protein.[6] Furthermore, several nucleotide substitutions in the promoter region of the MBL2 gene at position −550 (H/L polymorphism), −221 (X/Y polymorphism) and −427, −349, −336, del (−324 to −329), −70 and +4 (P/Q polymorphisms) affect the MBL serum concentration. Both the frequency of structural mutations and the promoter polymorphisms that are in strong linkage disequilibrium vary among ethnic groups resulting in seven major haplotypes: HYPA, LYQA, LYPA, LXPA, LYPB, LYQC and HYPD. Differences in the distribution of these haplotypes are the major cause of interracial variations in MBL serum levels. Both HYPA and LYQA are high-producing haplotypes, LYPA intermediate-producing haplotype and LXPA low-producing haplotype, whereas LYPB, LYQC and HYPD are defective haplotypes, which cause a severe MBL deficiency.[7]

Both MBL2 and MBL1P1 genes has been repeatedly hit throughout evolution of primates. The latter silenced eventually by mutations in the glycine residues of the collagen-like region. It has been selectively turned off during evolution through the same molecular mechanisms causing the MBL2 variant alleles in man, suggesting an evolutionary selection for low-producing MBL genes.[6]

Posttranslational modifications

In rat hepatocytes, MBL is synthesized in the rough endoplasmic reticulum. While in Golgi, it undergoes two distinct posttranslational modifications and is assembled into high molecular weight multimeric complexes. The modifications produce MBL in multiple forms of slightly various molecular masses and pI from 5.7 to 6.2.[8] Proteolytic cleavage also resulted in removal of the 20-aa N-terminal signal peptide,[9] and hydroxylation and glycosylation were also detected.[8] Some cysteine residues can be converted to dehydroalanin.[10]

Function

MBL belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding of MBL to a micro-organism results in activation of the lectin pathway of the complement system.

Another important function of MBL is that this molecule binds senescent[11] and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.[12][13]

Activation

The complement system can be activated through three pathways: the classical pathway, the alternative pathway, and the lectin pathway. One way that the most-recently discovered lectin pathway is activated is through mannose-binding lectin protein. MBL binds to carbohydrates (to be specific, D-mannose and L-fucose residues) found on the surfaces of many pathogens.

For example, MBL has been shown to bind to:

Complexes

MBL in the blood is complexed with (bound to) another protein, a serine protease called MASP (MBL-associated serine protease). There are three MASPs: MASP-1, MASP-2 and MASP-3, which have protease domains. There are also sMAP (also called MAp19) and MAp44, which do not have protease domains and are thought to be regulatory molecules of MASPs. MASPs also form complexes with ficolins, which are similar to MBL functionally and structurally with the exception that ficolins recognize their targets through fibrinogen-like domains, unlike MBL.

In order to activate the complement system when MBL binds to its target (for example, mannose on the surface of a bacterium), the MASP protein functions to cleave the blood protein C4 into C4a and C4b. The C4b fragments can then bind to the surface of the bacterium, and initiate the formation of a C3-convertase.

The subsequent complement cascade catalyzed by C3-convertase results in creating a membrane attack complex, which causes lysis of the pathogen as well as altered-self in the context of apoptotic and necrotic cells.

MBL/MASP-1 complex also has thrombin-like activity (thrombin clots fibrin to initiate blood clots). Mice that genetically lack MBL or MASP-1/3 (but not MASP-2/sMAP) have prolonged bleeding time in experimental injury models, although mice are seen to be normal if there is no insult to the body.

Clinical significance

It is produced in the liver as a response to infection, and is part of many other factors termed acute phase proteins.[17] Expression and function in other organs were also suggested.[18] The three structural polymorphisms of exon 1 have been reported to cause susceptibility to various common infections, including meningococcal disease.[19][20] However, evidence has been presented that suggests no harmful effect of these variants with regard to mengingococcal disease.[21]

External links

References

  1. ↑ Fraser IP, Koziel H, Ezekowitz RA (1998). "The serum mannose-binding protein and the macrophage mannose receptor are pattern recognition molecules that link innate and adaptive immunity". Semin. Immunol. 10 (5): 363–72. doi:10.1006/smim.1998.0141. PMID 9799711.
  2. ↑ Worthley DL, Bardy PG, Mullighan CG (2005). "Mannose-binding lectin: biology and clinical implications". Internal medicine journal. 35 (9): 548–55. doi:10.1111/j.1445-5994.2005.00908.x. PMID 16105157.
  3. ↑ Sheriff S, Chang CY, Ezekowitz RA (November 1994). "Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple alpha-helical coiled-coil". Nat. Struct. Biol. 1 (11): 789–94. doi:10.1038/nsb1194-789. PMID 7634089.
  4. ↑ Sastry K, Herman GA, Day L, Deignan E, Bruns G, Morton CC, Ezekowitz RA (October 1989). "The human mannose-binding protein gene. Exon structure reveals its evolutionary relationship to a human pulmonary surfactant gene and localization to chromosome 10". J. Exp. Med. 170 (4): 1175–89. doi:10.1084/jem.170.4.1175. PMC 2189467. PMID 2477486.
  5. ↑ Guo N, Mogues T, Weremowicz S, Morton CC, Sastry KN (March 1998). "The human ortholog of rhesus mannose-binding protein-A gene is an expressed pseudogene that localizes to chromosome 10". Mamm. Genome. 9 (3): 246–9. doi:10.1007/s003359900735. PMID 9501312.
  6. ↑ 6.0 6.1 Seyfarth J, Garred P, Madsen HO (2005). "The 'involution' of mannose-binding lectin". Human Molecular Genetics. 14 (19): 2859–69. doi:10.1093/hmg/ddi318. PMID 16115813.
  7. ↑ Online Mendelian Inheritance in Man (OMIM) mannose-binding protein deficiency -614372
  8. ↑ 8.0 8.1 Colley KJ, Baenziger JU (1987). "Identification of the posttranslational modifications of the core-specific lectin. The core-specific lectin contains hydroxyproline, hydroxylysine, and glucosylgalactosylhydroxylysine residues". J Biol Chem. 262 (21): 10290–5. PMID 3611062.
  9. ↑ "Mannose-binding protein C precursor [Homo sapiens]". Retrieved 2012-01-03.
  10. ↑ Jensen PH, Laursen I, Matthiesen F, Højrup P (2007). "Posttranslational modifications in human plasma MBL and human recombinant MBL". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1774: 335–44. doi:10.1016/j.bbapap.2006.12.008.
  11. ↑ Tomaiuolo R, Ruocco A, Salapete C, Carru C, Baggio G, Franceschi C, Zinellu A, Vaupel J, Bellia C, Lo Sasso B, Ciaccio M, Castaldo G, Deiana L (March 2012). "Activity of mannose-binding lectin (MBL) in centenarians". Aging Cell. 11 (3): 394–400. doi:10.1111/j.1474-9726.2012.00793.x. PMID 22239660.
  12. ↑ Ogden CA, deCathelineau A, Hoffmann PR, Bratton D, Ghebrehiwet B, Fadok VA, Henson PM (September 2001). "C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells". J. Exp. Med. 194 (6): 781–95. doi:10.1084/jem.194.6.781. PMC 2195958. PMID 11560994.
  13. ↑ Stuart LM, Takahashi K, Shi L, Savill J, Ezekowitz RA (March 2005). "Mannose-binding lectin-deficient mice display defective apoptotic cell clearance but no autoimmune phenotype". J. Immunol. 174 (6): 3220–6. doi:10.4049/jimmunol.174.6.3220. PMID 15749852.
  14. ↑ Choteau, L; Parny, M; François, N; Bertin, B; Fumery, M; Dubuquoy, L; Takahashi, K; Colombel, J-F; Jouault, T; Poulain, D; Sendid, B; Jawhara, S (7 October 2015). "Role of mannose-binding lectin in intestinal homeostasis and fungal elimination". Mucosal Immunology. 9 (3): 767–776. doi:10.1038/mi.2015.100. ISSN 1933-0219.
  15. ↑ de Jong MA, Vriend LE, Theelen B, Taylor ME, Fluitsma D, Boekhout T, Geijtenbeek TB (March 2010). "C-type lectin Langerin is a beta-glucan receptor on human Langerhans cells that recognizes opportunistic and pathogenic fungi". Mol. Immunol. 47 (6): 1216–25. doi:10.1016/j.molimm.2009.12.016. PMC 2837148. PMID 20097424.
  16. ↑ Ji X, Gewurz H, Spear GT (February 2005). "Mannose binding lectin (MBL) and HIV". Mol. Immunol. 42 (2): 145–52. doi:10.1016/j.molimm.2004.06.015. PMID 15488604.
  17. ↑ Herpers, B L; Endeman, H; de Jong, B A W; de Jongh, B M; Grutters, J C; Biesma, D H; vam Velzen-Blad, H (Jun 2009). "Acute-phase responsiveness of mannose-binding lectin in community-acquired pneumonia is highly dependent upon MBL2 genotypes". Clin Exp Immunol. 156 (3): 488–94. doi:10.1111/j.1365-2249.2009.03929.x. PMC 2691978. PMID 19438602.
  18. ↑ Worthley DL, Bardy PG, Gordon DL, Mullighan CG (October 2006). "Mannose-binding lectin and maladies of the bowel and liver". World J. Gastroenterol. 12 (40): 6420–8. PMID 17072973.
  19. ↑ Hibberd, M. L.; Sumiya, M.; Summerfield, J. A.; Booy, R.; Levin, M. (1999). "Association of variants of the gene for mannose-binding lectin with susceptibility to meningococcal disease". The Lancet. 353 (9158): 1049. doi:10.1016/S0140-6736(98)08350-0.
  20. ↑ Faber, J.; Schuessler, T.; Finn, A.; Murdoch, C.; Zenz, W.; Habermehl, P.; Meyer, C. U.; Zabel, B. U.; Schmitt, H. J.; Zepp, F.; Knuf, M. (2007). "Age-Dependent Association of Human Mannose-Binding Lectin Mutations with Susceptibility to Invasive Meningococcal Disease in Childhood". The Pediatric Infectious Disease Journal. 26 (3): 243–246. doi:10.1097/01.inf.0000256751.76218.7c. PMID 17484222.
  21. ↑ Bradley, D. T.; Bourke, T. W.; Fairley, D. J.; Borrow, R.; Shields, M. D.; Young, I. S.; Zipfel, P. F.; Hughes, A. E. (2012). "Genetic susceptibility to invasive meningococcal disease: MBL2 structural polymorphisms revisited in a large case-control study and a systematic review". International Journal of Immunogenetics: no. doi:10.1111/j.1744-313X.2012.01095.x.
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