Concentration
WikiDoc Resources for Concentration 
Articles 

Most recent articles on Concentration Most cited articles on Concentration 
Media 
Powerpoint slides on Concentration 
Evidence Based Medicine 
Clinical Trials 
Ongoing Trials on Concentration at Clinical Trials.gov Trial results on Concentration Clinical Trials on Concentration at Google

Guidelines / Policies / Govt 
US National Guidelines Clearinghouse on Concentration NICE Guidance on Concentration

Books 
News 
Commentary 
Definitions 
Patient Resources / Community 
Patient resources on Concentration Discussion groups on Concentration Patient Handouts on Concentration Directions to Hospitals Treating Concentration Risk calculators and risk factors for Concentration

Healthcare Provider Resources 
Causes & Risk Factors for Concentration 
Continuing Medical Education (CME) 
International 

Business 
Experimental / Informatics 
EditorInChief: C. Michael Gibson, M.S., M.D. [1]
Overview
In chemistry, concentration is the measure of how much of a given substance there is mixed with another substance. This can apply to any sort of chemical mixture, but most frequently the concept is limited to homogeneous solutions, where it refers to the amount of solute in a substance.
To concentrate a solution, one must add more solute, or reduce the amount of solvent (for instance, by selective evaporation). By contrast, to dilute a solution, one must add more solvent, or reduce the amount of solute.
Unless two substances are fully miscible there exists a concentration at which no further solute will dissolve in a solution. At this point, the solution is said to be saturated. If additional solute is added to a saturated solution, it will not dissolve (except in certain circumstances, when supersaturation may occur). Instead, phase separation will occur, leading to either coexisting phases or a suspension. The point of saturation depends on many variables such as ambient temperature and the precise chemical nature of the solvent and solute.
Analytical concentration includes all the forms of that substance in the solution.
Qualitative description
Often in informal, nontechnical language, concentration is described in a qualitative way, through the use of adjectives such as "dilute" or "weak" for solutions of relatively low concentration and of others like "concentrated" or "strong" for solutions of relatively high concentration. Those terms relate the amount of a substance in a mixture to the observable intensity of effects or properties caused by that substance. For example, a practical rule is that the more concentrated a chromatic solution is, the more intensely colored it is.
Quantitative notation
For scientific or technical applications, a qualitative account of concentration is almost never sufficient, therefore quantitative measures are needed to describe concentration. There are a number of different ways to quantitatively express concentration; the most common are listed below. They are based on mass or volume or both. Depending on what they are based on it is not always trivial to convert one measure to the other, because knowledge of the density might be needed to do so. At times this information may not be available, particularly if the temperature varies.
Mass versus volume
Some units of concentration particularly the most popular one (molarity) require knowledge of a substance's volume, which in contrast to mass is the variable depending on ambient temperature and pressure. In fact (partial) molar volume can even be a function of concentration itself. This is why volumes are not necessarily completely additive when two liquids are added and mixed. Volumebased measures for concentration are therefore not to be recommended for nondilute solutions or problems where relatively large differences in temperature are encountered (e.g. for phase diagrams).
Unless otherwise stated, all the following measurements of volume are assumed to be at a standard state temperature and pressure (for example 25 degrees Celsius at 1 atmosphere or 101.325 kPa). The measurement of mass does not require such restrictions.
Mass can be determined at a precision of < 0.2 mg on a routine basis with an analytical balance and more precise instruments exist. Both solids and liquids are easily quantified by weighing.
The volume of a liquid is usually determined by calibrated glassware such as burettes and volumetric flasks. For very small volumes precision syringes are available. The use of graduated beakers and cylinders is not recommended as their indication of volume is mostly for decorative rather than quantitative purposes. The volume of solids, particularly of powders, is often difficult to measure, which is why mass is the more usual measure. For gases the opposite is true, the volume of a gas can be measured in a gas burette, if care is taken to control the pressure. The mass is not so easy to measure due to buoyancy effects.
Molarity
 See also: Molar solution
Molarity (mol/L, molar, or (obsolete) M) denotes the number of moles of a given substance per liter of solution. M = mol/L. For instance:
 Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \frac{2.0 \text{ moles of dissolved particles}}{4.0 \text{ liters of liquid}} = \text{ solution of 0.5 mol/L}
Such a solution may be described as "0.50 molar." It must be emphasized that a 0.5 molar solution contains 0.5 moles of solute in 1.0 liter of solution. This is not equivalent to 1.0 liter of solvent. A 0.5 mol/L solution will contain either slightly more or slightly less than 1 liter of solvent because the process of dissolution causes the volume of the liquid to increase or decrease.
Following the SI system of units, the National Institute of Standards and Technology, the United States authority on measurement, considers the term molarity and the unit symbol M to be obsolete, and suggests instead the 'amountofsubstance concentration' (c) with units mol/m^{3} or other units used alongside the SI such as mol/L^{[1]}. This recommendation has not been universally implemented in academia yet.
Preparation of a solution of known molarity involves adding an accurately weighed amount of solute to a volumetric flask, adding some solvent to dissolve it, then adding more solvent to fill to the volume mark.
When discussing the molarity of minute concentrations, such as in a lot of pharmacological research, molarity is expressed in millimolars (mmol/L, mM, 1 thousandth of a molar), micromolars (μmol/L, μM, 1 millionth of a molar) or nanomolars (nmol/L, nM, 1 billionth of a molar).
Although molarity is by far the most commonly used measure for concentration, particularly for dilute aqueous solutions, it does suffer from a number of disadvantages. Masses can be determined with great precision as balances are often very precise. Determining volume is often not as precise. In addition, a volume of a liquid changes with temperature so that the molarity also changes without adding or removing any mass. For nondilute solutions another problem is that the molar volume of a substance is itself a function of concentration so that volume is not strictly additive.
Molality
Molality (mol/kg, molal, or m) denotes the number of moles of a given substance per kilogram of solvent (not solution). For instance: adding 1.0 moles of dissolved particles to 2.0 kilograms of solvent constitutes a solution with a molality of 0.5 mol/kg. Such a solution may be described as "0.5 molal". The term molal solution is used as a shorthand for a "one molal solution", i.e. a solution which contains one mole of the solute per 1000 grams of the solvent.
Following the SI system of units, the National Institute of Standards and Technology, the United States authority on measurement, considers the unit symbol m to be obsolete, and suggests instead the term 'molality of substance B' (m_{B}) with units mol/kg or a related unit of the SI^{[2]}. This recommendation has not been universally implemented in academia yet.
Note that molality is sometimes represented by the symbol (m), while molarity by the symbol (M). The two symbols are not meant to be confused, and should not be used as symbols for units. The SI unit for molality is mol/kg. (The unit m means meter.)
The determination of molality only requires a good balance, because the masses of both solvent and solute can be obtained by weighing. Using a balance is often more precise than working with volumetric flasks burettes and pipettes. Another advantage of molality is that it does not change with the temperature as it deals with the mass of solvent, rather than the volume of solution. Volume typically increases with increase in temperature resulting in decrease in molarity. Molality of a solution is always constant irrespective of the physical conditions like temperature and pressure.
In a dilute aqueous solution near room temperature and standard atmospheric pressure, the molarity and molality will be very similar in value. This is because 1 kg of water roughly corresponds to a volume of 1 L at these conditions, and because the solution is dilute, the addition of the solute makes a negligible impact on the volume of the solution.
However, in all other conditions, this is usually not the case.
Mole fraction
The mole fraction Χ, (also called molar fraction) denotes the number of moles of solute as a proportion of the total number of moles in a solution. For instance: 1 mole of solute dissolved in 9 moles of solvent has a mole fraction of 1/10 or 0.1. Mole fractions are dimensionless quantities. (The mole percentage or molar percentage, denoted "mol %" and equal to 100% times the mole fraction, is sometimes quoted instead of the mole fraction.)
This measure is used very frequently in the construction of phase diagrams. It has a number of advantages:
 the measure is not temperature dependent (such as molarity) and does not require knowledge of the densities of the phase(s) involved
 a mixture of known mole fraction can be prepared by weighing off the appropriate masses of the constituents
 the measure is symmetrical: in the mole fractions Χ=0.1 and Χ=0.9, the roles of 'solvent' and 'solute' are reversed.
As both mole fractions and molality are only based on the masses of the components it is easy to convert between these measures. This is not true for molarity, which requires knowledge of the density.
Mass percentage
Mass percentage denotes the mass of a substance in a mixture as a percentage of the mass of the entire mixture. For instance: if a bottle contains 40 grams of ethanol and 60 grams of water, then it contains 40% ethanol by mass. Commercial concentrated aqueous reagents such as acids and bases are often labeled in concentrations of weight percentage with the specific gravity also listed. In older texts and references this is sometimes referred to as weightweight percentage (abbreviated as w/w). In water pollution chemistry, a common term of measuring total mass percentage of dissolved solids in an aqueous medium is total dissolved solids.
Massvolume percentage
Massvolume percentage, (sometimes referred to as weightvolume percentage or percent weight per volume and often abbreviated as % m/v or % w/v) describes the mass of the solute in g per 100 mL of the resulting solution. Massvolume percentage is often used for solutions made from a solid solute dissolved in a liquid. For example, a 40% w/v sugar solution contains 40 g of sugar per 100 mL of resulting solution.
Massvolume ratio
Often used in medicine and pharmacology, a ratio of the weight of a drug dissolved in a volume of water, is presented as, grams of solute : mL of water. Practitioners use the term "dilution" when referring to this arcane unit. The most ubiquitous example is epinephrine solutions where a 1:100,000 solution has 1 g epinephrine in 100,000 mL water. This is equivalent to 0.01 g/L epinephrine solution.
When the volume considered is a gas a specific approach is needed: the gas' pressure and temperature conditions must be considered. A typical use is in air pollution emission quantification. It is very common to find values such as 50 g/Nm^{3} or 50 g/m^{3}N. The "N" before or after the cubic meter indicates that the gas is under the Standard conditions for temperature and pressure.
Volumevolume percentage
Volumevolume percentage (sometimes referred to as percent volume per volume and abbreviated as % v/v) describes the volume of the solute in mL per 100 mL of the resulting solution. This is most useful when a liquid  liquid solution is being prepared. For example, a 40% v/v ethanol solution contains 40 mL ethanol per 100 mL total volume.
Normality
Normality highlights the chemical nature of salts: in solution, salts dissociate into distinct reactive species (ions such as H^{+}, Fe^{3+}, or Cl^{}). Normality accounts for any discrepancy between the concentrations of the various ionic species in a solution. For example, in a salt such as MgCl_{2}, there are two moles of Cl^{} for every mole of Mg^{+}, so the concentration of Cl^{} is said to be 2 N (read: "two normal"), compared to Mg^{+} which is 1 N (read: "one normal"). Further examples are given below.
Definition:
A normal is one gram equivalent of a solute per liter of solution. The definition of a gram equivalent varies depending on the type of chemical reaction that is discussed  it can refer to acids, bases, redox species, and ions that will precipitate.
Usage:
It is critical to note that normality measures a single ion which takes part in an overall solute. For example, one could determine the normality of hydroxide or sodium in an aqueous solution of sodium hydroxide, but the normality of sodium hydroxide itself has no meaning. Nevertheless it is often used to describe solutions of acids or bases, in those cases it is implied that the normality refers to the H^{+} or OH^{−} ion. For example, 2 Normal sulfuric acid (H_{2}SO_{4}), means that the normality of H^{+} ions is 2, or that the molarity of the sulfuric acid is 1. Similarly for 1 Molar H_{3}PO_{4} the normality is 3 as it contains three H^{+} ions.
Specific cases:
As ions in solution can react through different pathways, there are three common definitions for normality as a measure of reactive species in solution:
 In acidbase chemistry, normality is used to express the concentration of protons or hydroxide ions in the solution. Here, the normality differs from the molarity by an integer value  each solute can produce n equivalents of reactive species when dissolved. For example: 1 M aqueous Ca(OH)_{2} is 2 N (normal) in hydroxide.
 In redox reactions, normality measures the quantity of oxidizing or reducing agent that can accept or furnish one mole of electrons. Here, the normality scales from the molarity, most commonly, by a fractional value. Calculating the normality of redox species in solution can be challenging.
 In precipitation reactions, normality measures the concentration of ions which will precipitate in a given reaction. Here, the normality scales from the molarity again by an integer value.
Practical uses:
The measure of normality is extremely useful for titrations  given two species that are known to react with a known ratio, one simply needs to scale the volumes of solutions with known normalities to get a complete reaction with the following equation:
N_{a}V_{a}=N_{b}V_{b}
However, normality cannot reliably represent an unambiguous measure of the concentration of a solution. Since the measure of normality depends on the reaction that the solute participates in, the same concentration of solute can possess two different normalities for two different reactions. For example, Mg^{2+} is 2 N with respect to a Cl^{} ion, but it is only 1 N with respect to an O^{2} ion.
Accordingly, normality is no longer used to represent the concentration of a solution as such. Instead, a solution should be labeled according to its molarity, and it is then possible to calculate the normality for a particular titration using the equation above. NIST has also stipulated that this unit is obsolete and recommends discontinuing its use.
Formal
The formal (F) is yet another measure of concentration similar to molarity. Formal concentrations are sometimes used when solving chemical equilibrium problems. It is calculated based on the formula weights of chemicals per liter of solution. The difference between formal and molar concentrations is that the formal concentration indicates moles of the original chemical formula in solution, without regard for the species that actually exist in solution. Molar concentration, on the other hand, is the concentration of species in solution.
For example: if one dissolves sodium carbonate (Na_{2}CO_{3}) in a litre of water, the compound dissociates into the Na^{+} and CO_{3}^{2} ions. Some of the CO_{3}^{2} reacts with the water to form HCO_{3}^{} and H_{2}CO_{3}. If the pH of the solution is low, there is practically no Na_{2}CO_{3} left in the solution. So, although we have added 1 mol of Na_{2}CO_{3} to the solution, it does not contain 1 M of that substance. (Rather, it contains a molarity based on the other constituents of the solution.) However, it was once said that such solutions contain 1 F of Na_{2}CO_{3}.
"Partsper" notation
The partsper notation is used in some areas of science and engineering because it does not require conversion from weights or volumes to more chemically relevant units such as normality or molarity. It describes the amount of one substance in another. It is the ratio of the amount of the substance of interest to the amount of that substance plus the amount of the substance it is in.
 Parts per hundred (denoted by '%' [the per cent symbol], and very rarely 'pph')  denotes the amount of a given substance in a total amount of 100 regardless of the units of measure as long as they are the same. e.g. 1 gram per 100 gram. 1 part in 10^{2}.
 Parts per thousand (denoted by '‰' [the per mille symbol], and occasionally 'ppt', though this should be avoided) denotes the amount of a given substance in a total amount of 1000 regardless of the units of measure as long as they are the same. e.g. 1 milligram per gram, or 1 gram per kilogram. 1 part in 10^{3}.
 Parts per million ('ppm') denotes the amount of a given substance in a total amount of 1,000,000 regardless of the units of measure used as long as they are the same. e.g. 1 milligram per kilogram. 1 part in 10^{6}.
 Parts per billion ('ppb') denotes the amount of a given substance in a total amount of 1,000,000,000 regardless of the units of measure as long as they are the same. e.g. 1 milligram per tonne. 1 part in 10^{9}.
 Parts per trillion ('ppt') denotes the amount of a given substance in a total amount of 1,000,000,000,000 regardless of the units of measure as long as they are the same. e.g. 1 milligram per kilotonne. 1 part in 10^{12}.
 Parts per quadrillion ('ppq') denotes the amount of a given substance in a total amount of 1,000,000,000,000,000 regardless of the units of measure as long as they are the same. e.g. 1 milligram per megatonne. 1 part in 10^{15}. There are currently no analytical techniques that can measure ppq concentrations.
According to the U.S. National Institute of Standards and Technology (NIST) Guide for the Use of the International System of Units (SI), "the languagedependent terms part per million, part per billion, and part per trillion ... are not acceptable for use with the SI to express the values of quantities."^{[3]}
Notes for clarity:
The notation is used for convenience and the units of measure must be denoted for clarity though this is frequently not the case even in technical publications.
In atmospheric chemistry and in air pollution regulations, the parts per notation is commonly expressed with a v following, such as ppmv, to indicate parts per million by volume. This works fine for gas concentrations (e.g., ppmv of carbon dioxide in the ambient air) but, for concentrations of nongaseous substances such as aerosols, cloud droplets, and particulate matter in the ambient air, the concentrations are commonly expressed as μg/m³ or mg/m³ (e.g., μg or mg of particulates per cubic metre of ambient air). This expression eliminates the need to take into account the impact of temperature and pressure on the density and hence weight of the gas.
The usage is generally quite fixed inside most specific branches of science, leading some researchers to believe that their own usage (mass/mass, volume/volume or others) is the only correct one. This, in turn, leads them not to specify their usage in their research, and others may therefore misinterpret their results. For example, electrochemists often use volume/volume, while chemical engineers may use mass/mass as well as volume/volume. Many academic papers of otherwise excellent level fail to specify their usage of the partper notation. The difference between expressing concentrations as mass/mass or volume/volume is quite significant when dealing with gases and it is very important to specify which is being used. It is quite simple, for example, to distinguish ppm by volume from ppm by mass or weight by using ppmv or ppmw.
Table of concentration measures
Measurement  Notation  Generic formula  Typical units 

atomic percentage (A)  at.%  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\rm number~of~atoms~of~dopant \times 100}{\rm number~of~atoms~of~solution} \right )  % 
atomic percentage (B)  at.%  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\rm number~of~atoms~of~dopant \times 100}{\rm number~of~substitutable~atoms~of~solution} \right )  % 
Mass percentage    Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{grams\ solute} \times 100}{\mathrm{grams\ solution}} \right )  % 
Massvolume percentage    Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{grams\ solute} \times 100}{\mathrm{milliliters\ solution}} \right )  % though strictly %g/mL 
Volumevolume percentage    Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{millilitres\ solute} \times 100}{\mathrm{milliliters\ solution}} \right )  % 
Molarity  M  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{moles\ solute}}{\mathrm{liters\ solution}} \right )  mol/L (or M or mol/dm^{3}) 
Molinity    Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{moles\ solute}}{\mathrm{kilograms\ solution}} \right )  mol/kg 
Molality  m  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{moles\ solute}}{\mathrm{kilograms\ solvent}} \right )  mol/kg (or m**) 
Molar fraction  Χ (chi)  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{moles\ solute}}{\mathrm{moles\ solution}} \right )  (decimal) 
Formal  F  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{moles\ undissolved\ solute}}{\mathrm{liters\ solution}} \right )  mol/L (or F) 
Normality  N  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{gram\ equivalents}}{\mathrm{liters\ solution}} \right )  N 
Parts per hundred  % (or pph)  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{dekagrams\ solute}}{\mathrm{kilograms\ solution}} \right )  da.g/kg 
Parts per thousand  ‰ (or ppt*)  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{grams\ solute}}{\mathrm{kilograms\ solution}} \right )  g/kg 
Parts per million  ppm  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{milligrams\ solute}}{\mathrm{kilograms\ solution}} \right )  mg/kg 
Parts per billion  ppb  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{micrograms\ solute}}{\mathrm{kilograms\ solution}} \right )  µg/kg 
Parts per trillion  ppt*  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{nanograms\ solute}}{\mathrm{kilograms\ solution}} \right )  ng/kg 
Parts per quadrillion  ppq  Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): \left ( \frac{\mathrm{picograms\ solute}}{\mathrm{kilograms\ solution}} \right )  pg/kg 
* Although 'ppt' is usually used to denote 'parts per trillion', it is on occasion used for 'parts per thousand'. Sometimes 'ppt' is also used as an abbreviation for precipitate.
** Obsolete unit symbols.
See also
References
 ↑ "NIST Guide to SI Units". Retrieved 20070903.
 ↑ "NIST Guide to SI Units". Retrieved 20071217.
 ↑ "NIST Guide to SI Units  Rules and Style Conventions for Expressing Values of Quantities". Retrieved 20070903.
 Note (1) : The table above is described in terms of solvents and solutes; however the units given often also apply to other types of mixture.
 Note (2) : The use of billion, trillion, and quadrillion above follows the short scale usage of these words.
cs:Koncentrace (chemie) de:Stoffkonzentration hu:Koncentráció io:Koncentro it:Concentrazione he:ריכוז (כימיה) mk:Концентрација nl:Concentratie (oplossing) no:Konsentrasjon nn:Konsentrasjon simple:Concentration sk:Koncentrácia sl:Koncentracija fi:Konsentraatio sv:Koncentration