Xylene cyanol

File:Agarose gel dyes.jpg

Xylene cyanol (XC) is most commonly used as an agarose gel electrophoresis size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than bromophenol blue (670g/mol) because of its charge (1 net negative charge).

• 10'000 bp at 0.7%
• 4000 bp at 1.5%
• 750 bp at 2.0%
• 200 bp at 3.0%

order information

• CAS 2650-17-1

• e.g. Sigma Xylene Cyanol FF X4126 10g at around €50

see also

• agarose gel loading buffer
• agarose gel electrophoresis
• Cresol Red - faster than XC
• Bromophenol blue - faster than XC

external links

• Xylene cyanol in the wikipedia