Streptomycin microbiology: Difference between revisions
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==Microbiology== | ==Microbiology== | ||
Streptomycin sulfate is a bactericidal antibiotic. It acts by interfering with normal protein synthesis. | Streptomycin sulfate is a [[bactericidal]] antibiotic. It acts by interfering with normal [[protein synthesis]]. | ||
Streptomycin has been shown to be active against most strains of the following organisms both in vitro and in clinical infection. (See INDICATIONS AND USAGE.): | Streptomycin has been shown to be active against most strains of the following organisms both in vitro and in clinical infection. (See INDICATIONS AND USAGE.): | ||
* [[Brucella]] ( | * [[Brucella]] ([[brucellosis]]) | ||
* [[ | * [[Calymmatobacterium granulomatis]] ([[donovanosis]], [[granuloma inguinale]]) | ||
* [[ | * [[Escherichia coli]] in [[urinary tract infection]]s | ||
* [[Klebsiella pneumoniae]] in urinary tract | * [[Proteus]] spp. in [[urinary tract infection]]s | ||
* [[Aerobacter aerogenes]] in [[urinary tract infection]]s | |||
* [[Klebsiella pneumoniae]] in [[urinary tract infection]]s | |||
* [[Enterococcus faecalis]] in [[urinary tract infection]]s | |||
* [[Francisella tularensis]] | * [[Francisella tularensis]] | ||
* [[Haemophilus ducreyi]] (chancroid) | |||
* [[Haemophilus ducreyi]] ([[chancroid]]) | |||
* [[Haemophilus influenzae]] (in respiratory, endocardial, and meningeal infections - concomitantly with another antibacterial agent) | * [[Haemophilus influenzae]] (in respiratory, endocardial, and meningeal infections - concomitantly with another antibacterial agent) | ||
* [[Klebsiella pneumoniae]] pneumonia (concomitantly with another antibacterial agent) | * [[Klebsiella pneumoniae]] pneumonia (concomitantly with another antibacterial agent) | ||
* [[Mycobacterium tuberculosis]] | * [[Mycobacterium tuberculosis]] | ||
* [[Pasteurella pestis]] | * [[Pasteurella pestis]] | ||
* [[Streptococcus viridans]] (in endocardial infections - concomitantly with penicillin) | * [[Streptococcus viridans]] (in endocardial infections - concomitantly with penicillin) | ||
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======Diffusion Techniques====== | ======Diffusion Techniques====== | ||
Quantitative methods that require measurement of zone diameters give the most precise estimate of the susceptibility of bacteria to antimicrobial agents. One such standard procedure 1 which has been recommended for use with disks to test susceptibility of organisms to streptomycin uses the 10 mcg streptomycin disk. Interpretation involves the correlation of the diameter obtained in the disk test with the minimum inhibitory concentration (MIC) for streptomycin. | Quantitative methods that require measurement of zone diameters give the most precise estimate of the susceptibility of bacteria to antimicrobial agents. One such standard procedure 1 which has been recommended for use with disks to test susceptibility of organisms to streptomycin uses the 10 mcg streptomycin disk. Interpretation involves the correlation of the diameter obtained in the disk test with the [[minimum inhibitory concentration]] (MIC) for streptomycin. | ||
Reports from the laboratory giving results of the standard single disk susceptibility test with a 10 mcg streptomycin disk should be interpreted according to the following criteria: | Reports from the laboratory giving results of the standard single disk susceptibility test with a 10 mcg streptomycin disk should be interpreted according to the following criteria: | ||
'''''Interpretive Criteria for Enterobacteriaceae''''' | '''''Interpretive Criteria for Enterobacteriaceae''''' | ||
{| | {| | ||
| [[File:STREPTOMYCIN02.jpg|400px|thumb]] | | [[File:STREPTOMYCIN02.jpg|400px|thumb]] | ||
Latest revision as of 17:54, 7 January 2014
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Microbiology
Streptomycin sulfate is a bactericidal antibiotic. It acts by interfering with normal protein synthesis.
Streptomycin has been shown to be active against most strains of the following organisms both in vitro and in clinical infection. (See INDICATIONS AND USAGE.):
- Proteus spp. in urinary tract infections
- Haemophilus influenzae (in respiratory, endocardial, and meningeal infections - concomitantly with another antibacterial agent)
- Klebsiella pneumoniae pneumonia (concomitantly with another antibacterial agent)
- Streptococcus viridans (in endocardial infections - concomitantly with penicillin)
- Enterococcus faecalis (in endocardial infections - concomitantly with penicillin)
Susceptibility Tests
Diffusion Techniques
Quantitative methods that require measurement of zone diameters give the most precise estimate of the susceptibility of bacteria to antimicrobial agents. One such standard procedure 1 which has been recommended for use with disks to test susceptibility of organisms to streptomycin uses the 10 mcg streptomycin disk. Interpretation involves the correlation of the diameter obtained in the disk test with the minimum inhibitory concentration (MIC) for streptomycin.
Reports from the laboratory giving results of the standard single disk susceptibility test with a 10 mcg streptomycin disk should be interpreted according to the following criteria:
Interpretive Criteria for Enterobacteriaceae
 |
A report of “Susceptible” indicates that the pathogen is likely to respond to monotherapy with streptomycin. A report of “Intermediate” indicates that the result be considered equivocal, and, if the organism is not fully susceptible to alternative clinically feasible drugs, the test should be repeated. This category provides a buffer zone which prevents small uncontrolled technical factors from causing major discrepancies in interpretations. A report of “Resistant” indicates that achievable drug concentrations are unlikely to be inhibitory and other therapy should be selected.
Standardized procedures require the use of laboratory control organisms. The 10 mcg streptomycin disk should give the following zone diameter:[1]
Acceptable Control Ranges
 |
Interpretive Criteria for Agents of Bioterrorism
 |
References
Adapted from the FDA Package Insert.