Mycoplasma pneumonia laboratory findings: Difference between revisions
YazanDaaboul (talk | contribs) No edit summary |
YazanDaaboul (talk | contribs) No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
''Mycoplasma'' pneumonia is diagnosed by either culture, serology, or molecular methods. Other laboratory findings include elevated CRP and/or ESR, leucocytosis, or hemolytic anemia. | |||
==Laboratory Findings== | ==Laboratory Findings== | ||
*''Mycoplasma'' pneumonia is diagnosed by either culture, serology, or molecular methods. | *''Mycoplasma'' pneumonia is diagnosed by either culture, serology, or molecular methods. | ||
| Line 18: | Line 19: | ||
| | | | ||
*Provides clinical isolates for genotyping and antimicrobial susceptibilities testing | *Provides clinical isolates for genotyping and antimicrobial susceptibilities testing | ||
*100% specificity when a positive result is obtained | *100% specificity when a positive result is obtained<sup>a</sup> | ||
*Commercially available SP4 media | *Commercially available SP4 media | ||
| | | | ||
| Line 29: | Line 30: | ||
|- | |- | ||
| '''Serology''' | | '''Serology''' | ||
|*Commercially available kits | | | ||
*Commercially available kits | |||
*Quantification possible | *Quantification possible | ||
| | | | ||
| Line 37: | Line 39: | ||
*Cumbersome and time-sensitive sampling | *Cumbersome and time-sensitive sampling | ||
*Not FDA approved | *Not FDA approved | ||
|*'''Clinical Service''': Sera provided to a clinical laboratory testing service for EIA testing | | | ||
*'''Clinical Service''': Sera provided to a clinical laboratory testing service for EIA testing | |||
|- | |- | ||
| '''Molecular''' | | '''Molecular''' | ||
| Line 53: | Line 56: | ||
*Limited FDA approval | *Limited FDA approval | ||
| | | | ||
*'''Clinical Service:''' NP, OP, or sputum provided to a clinical laboratory testing service for qPCR testing | *'''Clinical Service:''' NP, OP, or sputum provided to a clinical laboratory testing service for qPCR testing<sup>b</sup> | ||
*'''CDC''': Multiplex qPCR used for ''M. pneumoniae'' detection in NP, OP, sputum tissue, or CSF | *'''CDC''': Multiplex qPCR used for ''M. pneumoniae'' detection in NP, OP, sputum tissue, or CSF<sup>c,d</sup> | ||
*'''Commercial / FDA Approved''': 1) FilmArray (BioFire Inc) is a nested multiplex PCR in a closed "lab-in-a-pouch" format, 2) Illumigene (Meridian Biosciences, Inc) is a loop-mediated isothermal amplification (LAMP) assay | *'''Commercial / FDA Approved''': 1) FilmArray (BioFire Inc) is a nested multiplex PCR in a closed "lab-in-a-pouch" format, 2) Illumigene (Meridian Biosciences, Inc) is a loop-mediated isothermal amplification (LAMP) assay | ||
|} | |} | ||
<br> | |||
<SMALL><sup>a</sup>Provided that appropriate additional procedures are used to identify the organism isolated to species level. | |||
<sup>b</sup> State and local departments of public health may offer these diagnostic tests for the detection of M. pneumoniae. | |||
<sup>c</sup> Various quality control measures in place for this assay including compliance with Clinical Laboratory Improvement Amendments standards and the use of proficiency testing through Quality Control for Molecular Diagnostics. | |||
<sup>d</sup>Other testing performed by the CDC only for special investigations include: using specialized SP4 media used for isolation and isolate typing, serology as a supportive measure, and molecular typing procedures such as macrolide susceptibility testing, P1 adhesion typing, and MLVA typing.<br> | |||
Abbreviations: SP4—modified Spiroplasma medium, EIA - enzyme immunoassay, qPCR—quantitative polymerase chain reaction, MLVA—multiple-locus variable number tandem repeat analysis, NP—nasopharyngeal, OP—oropharyngeal, CSF—cerebrospinal fluid | |||
<br> | |||
Table adapted from the Centers for Disease Control and Prevention (CDC).<ref name=CDC> Mycoplasma pneumoniae infection - Centers for Disease Control and Prevention (CDC) http://www.cdc.gov/pneumonia/atypical/mycoplasma/hcp/diagnostic-methods.html#modalIdString_CDCTable_0 Accessed on Feb 10 2016</ref> | |||
</SMALL> | |||
==Other Laboratory Findings== | |||
Other laboratory findings that may be present among patients with ''Mycoplasma'' pneumonia include: | |||
*Elevated [[CRP]] and/or [[ESR]] | |||
*[[Leucocyte]] count may be normal or elevated | |||
*[[Hemolytic anemia]] | |||
==References== | ==References== | ||
{{Reflist|2}} | {{Reflist|2}} | ||
| Line 63: | Line 80: | ||
[[Category:Disease]] | [[Category:Disease]] | ||
[[Category:Pulmonology]] | [[Category:Pulmonology]] | ||
{{WH}} | {{WH}} | ||
{{WS}} | {{WS}} | ||
Revision as of 00:41, 8 February 2016
|
Mycoplasma pneumonia Microchapters |
|
Diagnosis |
|
Treatment |
|
Case Studies |
|
Mycoplasma pneumonia laboratory findings On the Web |
|
American Roentgen Ray Society Images of Mycoplasma pneumonia laboratory findings |
|
Risk calculators and risk factors for Mycoplasma pneumonia laboratory findings |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Overview
Mycoplasma pneumonia is diagnosed by either culture, serology, or molecular methods. Other laboratory findings include elevated CRP and/or ESR, leucocytosis, or hemolytic anemia.
Laboratory Findings
- Mycoplasma pneumonia is diagnosed by either culture, serology, or molecular methods.
- The following table demonstrates the advantages and disadvantages of each diagnostic method for Mycoplasma pneumonia:
| Method | Advantages | Disadvantages | Test Setting |
| Culture |
|
|
|
| Serology |
|
|
|
| Molecular |
|
|
|
aProvided that appropriate additional procedures are used to identify the organism isolated to species level.
b State and local departments of public health may offer these diagnostic tests for the detection of M. pneumoniae.
c Various quality control measures in place for this assay including compliance with Clinical Laboratory Improvement Amendments standards and the use of proficiency testing through Quality Control for Molecular Diagnostics.
dOther testing performed by the CDC only for special investigations include: using specialized SP4 media used for isolation and isolate typing, serology as a supportive measure, and molecular typing procedures such as macrolide susceptibility testing, P1 adhesion typing, and MLVA typing.
Abbreviations: SP4—modified Spiroplasma medium, EIA - enzyme immunoassay, qPCR—quantitative polymerase chain reaction, MLVA—multiple-locus variable number tandem repeat analysis, NP—nasopharyngeal, OP—oropharyngeal, CSF—cerebrospinal fluid
Table adapted from the Centers for Disease Control and Prevention (CDC).[1]
Other Laboratory Findings
Other laboratory findings that may be present among patients with Mycoplasma pneumonia include:
- Elevated CRP and/or ESR
- Leucocyte count may be normal or elevated
- Hemolytic anemia
References
- ↑ Mycoplasma pneumoniae infection - Centers for Disease Control and Prevention (CDC) http://www.cdc.gov/pneumonia/atypical/mycoplasma/hcp/diagnostic-methods.html#modalIdString_CDCTable_0 Accessed on Feb 10 2016