Mycoplasma pneumonia laboratory findings: Difference between revisions
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| Culture||"*Provides clinical isolates for genotyping and antimicrobial susceptibilities testing | | Culture||"*Provides clinical isolates for genotyping and antimicrobial susceptibilities testing | ||
*100% specificity when a positive result is obtained | |||
|- | |- | ||
| *Commercially available SP4 media"|| | |||
*Time-consuming | |||
| *Commercially available SP4 media"||*Time-consuming | |||
*Requires specialized expertise | *Requires specialized expertise | ||
*High potential for false negatives | *High potential for false negatives | ||
*Slow growth||*Reference laboratories only, not for routine diagnostics | *Slow growth||*Reference laboratories only, not for routine diagnostics | ||
|- | |- | ||
| Serology||*Commercially available kits | | Serology|| | ||
*Commercially available kits | |||
*Quantification possible||*Lacks specificity | *Quantification possible||*Lacks specificity | ||
*Need for acute and convalescent paired sera | *Need for acute and convalescent paired sera | ||
*Test results turn-around time not optimal for treatment decisions | *Test results turn-around time not optimal for treatment decisions | ||
*Cumbersome and time-sensitive sampling | *Cumbersome and time-sensitive sampling | ||
*Not FDA approved||*'''Clinical Service''': Sera provided to a clinical laboratory testing service for EIA testing | *Not FDA approved|| | ||
*'''Clinical Service''': Sera provided to a clinical laboratory testing service for EIA testing | |||
|- | |- | ||
| Molecular||*Commercially available kits | | Molecular|| | ||
*Commercially available kits | |||
*High sensitivity and specificity | *High sensitivity and specificity | ||
*Rapid detection | *Rapid detection | ||
*Results can be obtained in time to guide treatment decisions | *Results can be obtained in time to guide treatment decisions | ||
*Useful for strain typing||*Expensive | *Useful for strain typing|| | ||
*Expensive | |||
*Requires specialized expertise and equipment | *Requires specialized expertise and equipment | ||
*Not standardized | *Not standardized | ||
*Lack of clinical and comparative validation | *Lack of clinical and comparative validation | ||
*Limited FDA approval||*'''Clinical Service:''' NP, OP, or sputum provided to a clinical laboratory testing service for qPCR testing | *Limited FDA approval|| | ||
*'''Clinical Service:''' NP, OP, or sputum provided to a clinical laboratory testing service for qPCR testing | |||
*'''CDC''': Multiplex qPCR used for ''M. pneumoniae'' detection in NP, OP, sputum tissue, or CSF | *'''CDC''': Multiplex qPCR used for ''M. pneumoniae'' detection in NP, OP, sputum tissue, or CSF | ||
*'''Commercial / FDA Approved''': 1) FilmArray (BioFire Inc) is a nested multiplex PCR in a closed "lab-in-a-pouch" format, 2) Illumigene (Meridian Biosciences, Inc) is a loop-mediated isothermal amplification (LAMP) assay | *'''Commercial / FDA Approved''': 1) FilmArray (BioFire Inc) is a nested multiplex PCR in a closed "lab-in-a-pouch" format, 2) Illumigene (Meridian Biosciences, Inc) is a loop-mediated isothermal amplification (LAMP) assay |
Revision as of 00:29, 8 February 2016
Mycoplasma pneumonia Microchapters |
Diagnosis |
Treatment |
Case Studies |
Mycoplasma pneumonia laboratory findings On the Web |
American Roentgen Ray Society Images of Mycoplasma pneumonia laboratory findings |
Risk calculators and risk factors for Mycoplasma pneumonia laboratory findings |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Overview
Laboratory Findings
- Mycoplasma pneumonia is diagnosed by either culture, serology, or molecular methods.
- The following table demonstrates the advantages and disadvantages of each diagnostic method for Mycoplasma pneumonia:
Method | Advantages | Disadvantages | Test Setting |
Culture | "*Provides clinical isolates for genotyping and antimicrobial susceptibilities testing
| ||
*Commercially available SP4 media" |
| ||
Serology |
| ||
Molecular |
|