DNA replication factor CDT1: Difference between revisions

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<!-- The PBB_Controls template provides controls for Protein Box Bot, please see Template:PBB_Controls for details. -->
{{Infobox_gene}}
{{PBB_Controls
'''CDT1''' (Chromatin licensing and DNA replication factor 1) is a [[protein]] that in humans is encoded by the ''CDT1'' [[gene]].<ref name="pmid11896191">{{cite journal | vauthors = Rialland M, Sola F, Santocanale C | title = Essential role of human CDT1 in DNA replication and chromatin licensing | journal = J Cell Sci | volume = 115 | issue = Pt 7 | pages = 1435–40 |date=March 2002 | pmid = 11896191 | pmc =  | doi =  }}</ref><ref name="pmid11555648">{{cite journal | vauthors = Nishitani H, Taraviras S, Lygerou Z, Nishimoto T | title = The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase | journal = J Biol Chem | volume = 276 | issue = 48 | pages = 44905–11 |date=November 2001 | pmid = 11555648 | pmc =  | doi = 10.1074/jbc.M105406200 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: CDT1 chromatin licensing and DNA replication factor 1| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=81620| accessdate = }}</ref><ref>{{Cite web|url=https://ghr.nlm.nih.gov/gene/CDT1|title=CDT1 gene|last=Reference|first=Genetics Home|website=Genetics Home Reference|language=en|access-date=2018-07-19}}</ref> It is a licensing factor that functions to limit DNA from replicating more than once per cell cycle.
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<!-- The GNF_Protein_box is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
== Role in pre-replication complexes ==
{{GNF_Protein_box
The protein encoded by this gene is a key [[licensing factor]] in the assembly of [[pre-replication complex]]es (pre-RC), which occurs during the G1 phase of the cell cycle. In the assembly of pre-RCs, origin recognition complexes (ORC1-6) recognize and bind to DNA replication origins. CDT1, along with the protein [[Cdc6|CDC6]], are then recruited to the forming pre-RC, followed by minichromosome maintenance complexes (MCM2-7).<ref>{{Cite book|url=https://www.worldcat.org/oclc/1001961209|title=Hematology : basic principles and practice|last=|first=|publisher=|others=Hoffman, Ronald, 1945-, Benz, Edward J., Jr.,, Silberstein, Leslie E.,, Heslop, Helen,, Weitz, Jeffrey I.,, Anastasi, John,|year=|isbn=9780323509398|edition= Seventh|location=Philadelphia, PA|pages=Chapter 17, 176–185|oclc=1001961209}}</ref>
| image =
| image_source =
| PDB =
| Name = Chromatin licensing and DNA replication factor 1
| HGNCid = 24576
| Symbol = CDT1
| AltSymbols =; DUP; RIS2
| OMIM = 605525
| ECnumber = 
| Homologene = 32650
| MGIid = 1914427
| GeneAtlas_image1 = PBB_GE_CDT1_209832_s_at_tn.png
| Function = {{GNF_GO|id=GO:0003677 |text = DNA binding}} {{GNF_GO|id=GO:0005515 |text = protein binding}}
| Component = {{GNF_GO|id=GO:0005634 |text = nucleus}} {{GNF_GO|id=GO:0005657 |text = replication fork}}
| Process = {{GNF_GO|id=GO:0000076 |text = DNA replication checkpoint}} {{GNF_GO|id=GO:0006260 |text = DNA replication}} {{GNF_GO|id=GO:0007049 |text = cell cycle}} {{GNF_GO|id=GO:0007090 |text = regulation of S phase of mitotic cell cycle}} {{GNF_GO|id=GO:0030174 |text = regulation of DNA replication initiation}}
| Orthologs = {{GNF_Ortholog_box
    | Hs_EntrezGene = 81620
    | Hs_Ensembl = ENSG00000167513
    | Hs_RefseqProtein = NP_112190
    | Hs_RefseqmRNA = NM_030928
    | Hs_GenLoc_db = 
    | Hs_GenLoc_chr = 16
    | Hs_GenLoc_start = 87397687
    | Hs_GenLoc_end = 87403166
    | Hs_Uniprot = Q9H211
    | Mm_EntrezGene = 67177
    | Mm_Ensembl = ENSMUSG00000006585
    | Mm_RefseqmRNA = NM_026014
    | Mm_RefseqProtein = NP_080290
    | Mm_GenLoc_db = 
    | Mm_GenLoc_chr = 8
    | Mm_GenLoc_start = 125454116
    | Mm_GenLoc_end = 125459644
    | Mm_Uniprot = Q8R4E9
  }}
}}
'''Chromatin licensing and DNA replication factor 1''', also known as '''CDT1''', is a human [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: CDT1 chromatin licensing and DNA replication factor 1| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=81620| accessdate = }}</ref>


<!-- The PBB_Summary template is automatically maintained by Protein Box Bot. See Template:PBB_Controls to Stop updates. -->
The activity of CDT1 during the [[cell cycle]] is tightly regulated during the S phase by the protein [[geminin]], which inhibits its, and by SCF<sup>SKP2</sup>, which ubiquinates the protein to tag it for proteasomal degradation.<ref name="pmid11125146">{{cite journal|vauthors=Wohlschlegel JA, Dwyer BT, Dhar SK, Cvetic C, Walter JC, Dutta A|date=December 2000|title=Inhibition of eukaryotic DNA replication by geminin binding to Cdt1|url=|journal=Science|volume=290|issue=5500|pages=2309–12|doi=10.1126/science.290.5500.2309|pmid=11125146}}</ref> This regulation is important in preventing relicensing, thus ensuring that DNA is only replicated once per cell cycle.
{{PBB_Summary
 
| section_title =  
==Orthologs==
| summary_text =  
CDT1 belongs to a family of replication proteins conserved from yeast to humans. Examples of [[homology (biology)#Orthology|orthologs]] in other species include:
}}
* ''[[Schizosaccharomyces pombe|S. pombe]]'' – CDT1 (CDC10-dependent transcript 1)<ref name="pmid8313888">{{cite journal | vauthors = Hofmann JF, Beach D | title = cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis | journal = EMBO J. | volume = 13 | issue = 2 | pages = 425–34 |date=January 1994 | pmid = 8313888 | pmc = 394824 | url = | issn = }}</ref>
* ''[[Drosophila melanogaster]]'' – 'double parked' or Dup<ref name="pmid16531405">{{cite journal | vauthors = Nakajima H, Watanabe N, Shibata F, Kitamura T, Ikeda Y, Handa M | title = N-terminal region of CCAAT/enhancer-binding protein epsilon is critical for cell cycle arrest, apoptosis, and functional maturation during myeloid differentiation | journal = J. Biol. Chem. | volume = 281 | issue = 20 | pages = 14494–502 |date=May 2006 | pmid = 16531405 | doi = 10.1074/jbc.M600575200 | url =  }}</ref>
* ''[[African clawed frog|Xenopus laevis]]'' - CDT1<ref name="pmid10766247">{{cite journal | vauthors = Maiorano D, Moreau J, Méchali M | title = XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis. | journal = Nature | volume = 404 | pages = 622–5 |date=April 2000 | pmid = 10766247 | url = | issue = 6778 | doi = 10.1038/35007104 }}</ref>
 
== Interactions ==
DNA replication factor CDT1 has been shown to [[Protein-protein interaction|interact]] with [[SKP2]].<ref name="pmid12840033">{{cite journal | vauthors = Li X, Zhao Q, Liao R, Sun P, Wu X | title = The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation | journal = J. Biol. Chem. | volume = 278 | issue = 33 | pages = 30854–8 | year = 2003 | pmid = 12840033 | doi = 10.1074/jbc.C300251200 }}</ref> Cdt1 is recruited by the [[origin recognition complex]] in origin licensing. Null-mutations for CDT1 are lethal in yeast; the spores undergo mitosis without DNA replication. The overexpression of CDT1 causes rereplication in H. sapiens, which activates the CHK1 pathway, preventing entry into mitosis.<ref name="pmid15591064">{{cite journal | vauthors = Machida YJ, Dutta A | title = Cellular checkpoint mechanisms monitoring proper initiation of DNA replication | journal = J. Biol. Chem. | volume = 280 | issue = 8 | pages = 6253–6 | year = 2005 | pmid = 15591064 | doi = 10.1074/jbc.R400037200 }}</ref>


==References==
==References==
{{reflist|2}}
{{Reflist}}
 
==Further reading==
==Further reading==
{{refbegin | 2}}
{{refbegin | 2}}
{{PBB_Further_reading
*{{cite journal  | vauthors=Xouri G, Dimaki M, Bastiaens PI, Lygerou Z |title=Cdt1 interactions in the licensing process: a model for dynamic spatiotemporal control of licensing. |journal=Cell Cycle |volume=6 |issue= 13 |pages= 1549–52 |year= 2007 |pmid= 17598984 |doi=10.4161/cc.6.13.4455 }}
| citations =
*{{cite journal  | vauthors=Andersson B, Wentland MA, Ricafrente JY |title=A "double adaptor" method for improved shotgun library construction. |journal=Anal. Biochem. |volume=236 |issue= 1 |pages= 107–13 |year= 1996 |pmid= 8619474 |doi= 10.1006/abio.1996.0138 |display-authors=etal}}
*{{cite journal  | author=Xouri G, Dimaki M, Bastiaens PI, Lygerou Z |title=Cdt1 interactions in the licensing process: a model for dynamic spatiotemporal control of licensing. |journal=Cell Cycle |volume=6 |issue= 13 |pages= 1549-52 |year= 2007 |pmid= 17598984 |doi=  }}
*{{cite journal  | vauthors=Yu W, Andersson B, Worley KC |title=Large-scale concatenation cDNA sequencing. |journal=Genome Res. |volume=7 |issue= 4 |pages= 353–8 |year= 1997 |pmid= 9110174 |doi=  10.1101/gr.7.4.353| pmc=139146  |display-authors=etal}}
*{{cite journal  | author=Andersson B, Wentland MA, Ricafrente JY, ''et al.'' |title=A "double adaptor" method for improved shotgun library construction. |journal=Anal. Biochem. |volume=236 |issue= 1 |pages= 107-13 |year= 1996 |pmid= 8619474 |doi= 10.1006/abio.1996.0138 }}
*{{cite journal  | vauthors=Nishitani H, Lygerou Z, Nishimoto T, Nurse P |title=The Cdt1 protein is required to license DNA for replication in fission yeast. |journal=Nature |volume=404 |issue= 6778 |pages= 625–8 |year= 2000 |pmid= 10766248 |doi= 10.1038/35007110 }}
*{{cite journal  | author=Yu W, Andersson B, Worley KC, ''et al.'' |title=Large-scale concatenation cDNA sequencing. |journal=Genome Res. |volume=7 |issue= 4 |pages= 353-8 |year= 1997 |pmid= 9110174 |doi=  }}
*{{cite journal  | vauthors=Whittaker AJ, Royzman I, Orr-Weaver TL |title=Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. |journal=Genes Dev. |volume=14 |issue= 14 |pages= 1765–76 |year= 2000 |pmid= 10898791 |doi= | pmc=316778 }}
*{{cite journal  | author=Nishitani H, Lygerou Z, Nishimoto T, Nurse P |title=The Cdt1 protein is required to license DNA for replication in fission yeast. |journal=Nature |volume=404 |issue= 6778 |pages= 625-8 |year= 2000 |pmid= 10766248 |doi= 10.1038/35007110 }}
*{{cite journal  | vauthors=Wohlschlegel JA, Dwyer BT, Dhar SK |title=Inhibition of eukaryotic DNA replication by geminin binding to Cdt1. |journal=Science |volume=290 |issue= 5500 |pages= 2309–12 |year= 2001 |pmid= 11125146 |doi= 10.1126/science.290.5500.2309 |display-authors=etal}}
*{{cite journal  | author=Whittaker AJ, Royzman I, Orr-Weaver TL |title=Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. |journal=Genes Dev. |volume=14 |issue= 14 |pages= 1765-76 |year= 2000 |pmid= 10898791 |doi=  }}
*{{cite journal  | vauthors=Yanagi K, Mizuno T, You Z, Hanaoka F |title=Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity. |journal=J. Biol. Chem. |volume=277 |issue= 43 |pages= 40871–80 |year= 2002 |pmid= 12192004 |doi= 10.1074/jbc.M206202200 }}
*{{cite journal  | author=Wohlschlegel JA, Dwyer BT, Dhar SK, ''et al.'' |title=Inhibition of eukaryotic DNA replication by geminin binding to Cdt1. |journal=Science |volume=290 |issue= 5500 |pages= 2309-12 |year= 2001 |pmid= 11125146 |doi= 10.1126/science.290.5500.2309 }}
*{{cite journal  | vauthors=Bermejo R, Vilaboa N, Calés C |title=Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization. |journal=Mol. Biol. Cell |volume=13 |issue= 11 |pages= 3989–4000 |year= 2003 |pmid= 12429841 |doi= 10.1091/mbc.E02-04-0217 | pmc=133609 }}
*{{cite journal  | author=Nishitani H, Taraviras S, Lygerou Z, Nishimoto T |title=The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase. |journal=J. Biol. Chem. |volume=276 |issue= 48 |pages= 44905-11 |year= 2002 |pmid= 11555648 |doi= 10.1074/jbc.M105406200 }}
*{{cite journal  | vauthors=Strausberg RL, Feingold EA, Grouse LH |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899–903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899  | pmc=139241 |display-authors=etal}}
*{{cite journal  | author=Rialland M, Sola F, Santocanale C |title=Essential role of human CDT1 in DNA replication and chromatin licensing. |journal=J. Cell. Sci. |volume=115 |issue= Pt 7 |pages= 1435-40 |year= 2002 |pmid= 11896191 |doi=  }}
*{{cite journal  | vauthors=Li X, Zhao Q, Liao R |title=The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation. |journal=J. Biol. Chem. |volume=278 |issue= 33 |pages= 30854–8 |year= 2003 |pmid= 12840033 |doi= 10.1074/jbc.C300251200 |display-authors=etal}}
*{{cite journal  | author=Yanagi K, Mizuno T, You Z, Hanaoka F |title=Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity. |journal=J. Biol. Chem. |volume=277 |issue= 43 |pages= 40871-80 |year= 2002 |pmid= 12192004 |doi= 10.1074/jbc.M206202200 }}
*{{cite journal  | vauthors=Cook JG, Chasse DA, Nevins JR |title=The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells. |journal=J. Biol. Chem. |volume=279 |issue= 10 |pages= 9625–33 |year= 2004 |pmid= 14672932 |doi= 10.1074/jbc.M311933200 }}
*{{cite journal  | author=Bermejo R, Vilaboa N, Calés C |title=Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization. |journal=Mol. Biol. Cell |volume=13 |issue= 11 |pages= 3989-4000 |year= 2003 |pmid= 12429841 |doi= 10.1091/mbc.E02-04-0217 }}
*{{cite journal  | vauthors=Sugimoto N, Tatsumi Y, Tsurumi T |title=Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding. |journal=J. Biol. Chem. |volume=279 |issue= 19 |pages= 19691–7 |year= 2004 |pmid= 14993212 |doi= 10.1074/jbc.M313175200 |display-authors=etal}}
*{{cite journal  | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899-903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 }}
*{{cite journal  | vauthors=Liu E, Li X, Yan F |title=Cyclin-dependent kinases phosphorylate human Cdt1 and induce its degradation. |journal=J. Biol. Chem. |volume=279 |issue= 17 |pages= 17283–8 |year= 2004 |pmid= 15004027 |doi= 10.1074/jbc.C300549200 |display-authors=etal}}
*{{cite journal  | author=Li X, Zhao Q, Liao R, ''et al.'' |title=The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation. |journal=J. Biol. Chem. |volume=278 |issue= 33 |pages= 30854-8 |year= 2003 |pmid= 12840033 |doi= 10.1074/jbc.C300251200 }}
*{{cite journal  | vauthors=Ramachandran N, Hainsworth E, Bhullar B |title=Self-assembling protein microarrays. |journal=Science |volume=305 |issue= 5680 |pages= 86–90 |year= 2004 |pmid= 15232106 |doi= 10.1126/science.1097639 |display-authors=etal}}
*{{cite journal  | author=Cook JG, Chasse DA, Nevins JR |title=The regulated association of Cdt1 with minichromosome maintenance proteins and Cdc6 in mammalian cells. |journal=J. Biol. Chem. |volume=279 |issue= 10 |pages= 9625-33 |year= 2004 |pmid= 14672932 |doi= 10.1074/jbc.M311933200 }}
*{{cite journal  | vauthors=Ballabeni A, Melixetian M, Zamponi R |title=Human geminin promotes pre-RC formation and DNA replication by stabilizing CDT1 in mitosis. |journal=EMBO J. |volume=23 |issue= 15 |pages= 3122–32 |year= 2005 |pmid= 15257290 |doi= 10.1038/sj.emboj.7600314  | pmc=514931 |display-authors=etal}}
*{{cite journal  | author=Sugimoto N, Tatsumi Y, Tsurumi T, ''et al.'' |title=Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding. |journal=J. Biol. Chem. |volume=279 |issue= 19 |pages= 19691-7 |year= 2004 |pmid= 14993212 |doi= 10.1074/jbc.M313175200 }}
*{{cite journal  | vauthors=Saxena S, Yuan P, Dhar SK |title=A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. |journal=Mol. Cell |volume=15 |issue= 2 |pages= 245–58 |year= 2004 |pmid= 15260975 |doi= 10.1016/j.molcel.2004.06.045 |display-authors=etal}}
*{{cite journal  | author=Liu E, Li X, Yan F, ''et al.'' |title=Cyclin-dependent kinases phosphorylate human Cdt1 and induce its degradation. |journal=J. Biol. Chem. |volume=279 |issue= 17 |pages= 17283-8 |year= 2004 |pmid= 15004027 |doi= 10.1074/jbc.C300549200 }}
*{{cite journal  | vauthors=Kulartz M, Knippers R |title=The replicative regulator protein geminin on chromatin in the HeLa cell cycle. |journal=J. Biol. Chem. |volume=279 |issue= 40 |pages= 41686–94 |year= 2004 |pmid= 15284237 |doi= 10.1074/jbc.M405798200 }}
*{{cite journal  | author=Ramachandran N, Hainsworth E, Bhullar B, ''et al.'' |title=Self-assembling protein microarrays. |journal=Science |volume=305 |issue= 5680 |pages= 86-90 |year= 2004 |pmid= 15232106 |doi= 10.1126/science.1097639 }}
*{{cite journal  | vauthors=Beausoleil SA, Jedrychowski M, Schwartz D |title=Large-scale characterization of HeLa cell nuclear phosphoproteins. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=101 |issue= 33 |pages= 12130–5 |year= 2004 |pmid= 15302935 |doi= 10.1073/pnas.0404720101 | pmc=514446 |display-authors=etal}}
*{{cite journal  | author=Ballabeni A, Melixetian M, Zamponi R, ''et al.'' |title=Human geminin promotes pre-RC formation and DNA replication by stabilizing CDT1 in mitosis. |journal=EMBO J. |volume=23 |issue= 15 |pages= 3122-32 |year= 2005 |pmid= 15257290 |doi= 10.1038/sj.emboj.7600314 }}
*{{cite journal  | author=Saxena S, Yuan P, Dhar SK, ''et al.'' |title=A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. |journal=Mol. Cell |volume=15 |issue= 2 |pages= 245-58 |year= 2004 |pmid= 15260975 |doi= 10.1016/j.molcel.2004.06.045 }}
*{{cite journal | author=Kulartz M, Knippers R |title=The replicative regulator protein geminin on chromatin in the HeLa cell cycle. |journal=J. Biol. Chem. |volume=279 |issue= 40 |pages= 41686-94 |year= 2004 |pmid= 15284237 |doi= 10.1074/jbc.M405798200 }}
*{{cite journal  | author=Beausoleil SA, Jedrychowski M, Schwartz D, ''et al.'' |title=Large-scale characterization of HeLa cell nuclear phosphoproteins. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=101 |issue= 33 |pages= 12130-5 |year= 2004 |pmid= 15302935 |doi= 10.1073/pnas.0404720101 }}
}}
{{refend}}
{{refend}}


{{protein-stub}}
{{DNA replication}}


[[Category:Proteins]]
[[Category:Proteins]]
[[Category:DNA replication]]
[[Category:DNA replication]]
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Latest revision as of 09:25, 10 January 2019

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Identifiers
Aliases
External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez

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Ensembl

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UniProt

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RefSeq (mRNA)

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RefSeq (protein)

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Location (UCSC)n/an/a
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CDT1 (Chromatin licensing and DNA replication factor 1) is a protein that in humans is encoded by the CDT1 gene.[1][2][3][4] It is a licensing factor that functions to limit DNA from replicating more than once per cell cycle.

Role in pre-replication complexes

The protein encoded by this gene is a key licensing factor in the assembly of pre-replication complexes (pre-RC), which occurs during the G1 phase of the cell cycle. In the assembly of pre-RCs, origin recognition complexes (ORC1-6) recognize and bind to DNA replication origins. CDT1, along with the protein CDC6, are then recruited to the forming pre-RC, followed by minichromosome maintenance complexes (MCM2-7).[5]

The activity of CDT1 during the cell cycle is tightly regulated during the S phase by the protein geminin, which inhibits its, and by SCFSKP2, which ubiquinates the protein to tag it for proteasomal degradation.[6] This regulation is important in preventing relicensing, thus ensuring that DNA is only replicated once per cell cycle.

Orthologs

CDT1 belongs to a family of replication proteins conserved from yeast to humans. Examples of orthologs in other species include:

Interactions

DNA replication factor CDT1 has been shown to interact with SKP2.[10] Cdt1 is recruited by the origin recognition complex in origin licensing. Null-mutations for CDT1 are lethal in yeast; the spores undergo mitosis without DNA replication. The overexpression of CDT1 causes rereplication in H. sapiens, which activates the CHK1 pathway, preventing entry into mitosis.[11]

References

  1. ↑ Rialland M, Sola F, Santocanale C (March 2002). "Essential role of human CDT1 in DNA replication and chromatin licensing". J Cell Sci. 115 (Pt 7): 1435–40. PMID 11896191.
  2. ↑ Nishitani H, Taraviras S, Lygerou Z, Nishimoto T (November 2001). "The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase". J Biol Chem. 276 (48): 44905–11. doi:10.1074/jbc.M105406200. PMID 11555648.
  3. ↑ "Entrez Gene: CDT1 chromatin licensing and DNA replication factor 1".
  4. ↑ Reference, Genetics Home. "CDT1 gene". Genetics Home Reference. Retrieved 2018-07-19.
  5. ↑ Hematology : basic principles and practice. Hoffman, Ronald, 1945-, Benz, Edward J., Jr.,, Silberstein, Leslie E.,, Heslop, Helen,, Weitz, Jeffrey I.,, Anastasi, John, (Seventh ed.). Philadelphia, PA. pp. Chapter 17, 176–185. ISBN 9780323509398. OCLC 1001961209.
  6. ↑ Wohlschlegel JA, Dwyer BT, Dhar SK, Cvetic C, Walter JC, Dutta A (December 2000). "Inhibition of eukaryotic DNA replication by geminin binding to Cdt1". Science. 290 (5500): 2309–12. doi:10.1126/science.290.5500.2309. PMID 11125146.
  7. ↑ Hofmann JF, Beach D (January 1994). "cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis". EMBO J. 13 (2): 425–34. PMC 394824. PMID 8313888.
  8. ↑ Nakajima H, Watanabe N, Shibata F, Kitamura T, Ikeda Y, Handa M (May 2006). "N-terminal region of CCAAT/enhancer-binding protein epsilon is critical for cell cycle arrest, apoptosis, and functional maturation during myeloid differentiation". J. Biol. Chem. 281 (20): 14494–502. doi:10.1074/jbc.M600575200. PMID 16531405.
  9. ↑ Maiorano D, Moreau J, Méchali M (April 2000). "XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis". Nature. 404 (6778): 622–5. doi:10.1038/35007104. PMID 10766247.
  10. ↑ Li X, Zhao Q, Liao R, Sun P, Wu X (2003). "The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation". J. Biol. Chem. 278 (33): 30854–8. doi:10.1074/jbc.C300251200. PMID 12840033.
  11. ↑ Machida YJ, Dutta A (2005). "Cellular checkpoint mechanisms monitoring proper initiation of DNA replication". J. Biol. Chem. 280 (8): 6253–6. doi:10.1074/jbc.R400037200. PMID 15591064.

Further reading


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