Chronic myelogenous leukemia other diagnostic studies: Difference between revisions

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Reverse transcriptase (RT) PCR
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 Genomic PCR and Southern blot assay can determine the exact breakpoints of the fusion genes. Reverse transcriptase (RT) PCR and Northern blot analysis detect BCR-ABL transcripts at the RNA level. Western blot analysis or immunoprecipitation demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl (27).PMID:10428738 PMID:20221270
 Genomic PCR and Southern blot assay can determine the exact breakpoints of the fusion genes. Reverse transcriptase (RT) PCR and Northern blot analysis detect BCR-ABL transcripts at the RNA level. Western blot analysis or immunoprecipitation demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl (27).PMID:10428738 PMID:20221270
 Genomic PCR, Southern blot assay, Reverse transcriptase (RT) PCR, Northern blot analysis, Western blot analysis or immunoprecipitation can be helpful in the diagnosis of chronic myelogenous leukemia.


==Other diagnostic studies==
==Other diagnostic studies==
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:*Used to detect any metastasis to the [[cerebral spinal fluid]] (CSF)
:*Used to detect any metastasis to the [[cerebral spinal fluid]] (CSF)
*[[Lymph node biopsy]]
*[[Lymph node biopsy]]
:*Used to detect and/or determine the type of leukemic cells  
**Used to detect and/or determine the type of leukemic cells
*Genomic PCR
**can determine the exact breakpoints of the fusion genes
*Southern blot assay
**can determine the exact breakpoints of the fusion genes
*Reverse transcriptase (RT) PCR
**detect BCR-ABL transcripts at the RNA level
*Northern blot analysis
**detect BCR-ABL transcripts at the RNA level
*Western blot analysis
**demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl
*[[Immunoprecipitation]]
**demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl
:
:
:
==Gallery==
==Gallery==
<gallery widths="200px" class="center">
<gallery widths="200px" class="center">

Revision as of 18:20, 19 May 2018

Reverse transcriptase (RT) PCR


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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Mohamad Alkateb, MBBCh [2]

Overview

Other diagnostic studies for chronic myelogenous leukemia include bone marrow aspiration and biopsy, lumbar puncture, and lymph node biopsy.

cytogenetic studies for Ph and markers of karyotypic evolution. In patients who are Ph negative, molecular studies, such as Southern blot assay or polymerase chain reaction (PCR), are indicated to detect the BCR-ABL rearrangements.PMID:10428738

Cytogenetic analysis is the gold standard diagnostic test in chronic myelogenous leukemia.PMID:10428738

 Genomic PCR and Southern blot assay can determine the exact breakpoints of the fusion genes. Reverse transcriptase (RT) PCR and Northern blot analysis detect BCR-ABL transcripts at the RNA level. Western blot analysis or immunoprecipitation demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl (27).PMID:10428738 PMID:20221270

 Genomic PCR, Southern blot assay, Reverse transcriptase (RT) PCR, Northern blot analysis, Western blot analysis or immunoprecipitation can be helpful in the diagnosis of chronic myelogenous leukemia.

Other diagnostic studies

Other diagnostic studies for chronic myelogenous leukemia include:[1]

  • Used to detect and/or determine the type of leukemic cells
  • Lymph node biopsy
    • Used to detect and/or determine the type of leukemic cells
  • Genomic PCR
    • can determine the exact breakpoints of the fusion genes
  • Southern blot assay
    • can determine the exact breakpoints of the fusion genes
  • Reverse transcriptase (RT) PCR
    • detect BCR-ABL transcripts at the RNA level
  • Northern blot analysis
    • detect BCR-ABL transcripts at the RNA level
  • Western blot analysis
    • demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl
  • Immunoprecipitation
    • demonstrate the p210bcr-abl protein by using monoclonal antibodies against Bcr and Abl

Gallery

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