Monkeypox laboratory tests

Jump to navigation Jump to search

Monkeypox Microchapters

Home

Patient Info

Overview

Historical Perspective

Pathophysiology

Epidemiology & Demographics

Risk Factors

Screening

Causes of Monkeypox

Differentiating Monkeypox from other Diseases

Natural History, Complications & Prognosis

Diagnosis

History & Symptoms

Physical Examination

Lab Tests

Electrocardiogram

Chest X Ray

CT

MRI

Echocardiography or Ultrasound

Other Imaging Findings

Other Diagnostic Studies

Treatment

Medical Therapy

Surgery

Primary Prevention

Secondary Prevention

Cost-Effectiveness of Therapy

Future or Investigational Therapies

Monkeypox laboratory tests On the Web

Most recent articles

Most cited articles

Review articles

CME Programs

Powerpoint slides

Images

American Roentgen Ray Society Images of Monkeypox laboratory tests

All Images
X-rays
Echo & Ultrasound
CT Images
MRI

Ongoing Trials at Clinical Trials.gov

US National Guidelines Clearinghouse

NICE Guidance

FDA on Monkeypox laboratory tests

CDC on Monkeypox laboratory tests

Monkeypox laboratory tests in the news

Blogs on Monkeypox laboratory tests

Directions to Hospitals Treating Monkeypox

Risk calculators and risk factors for Monkeypox laboratory tests

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Bassel Almarie, M.D.

Overview

The laboratory findings of chickenpox include blood tests that can be done to identify the response to acute infection (IgM) or previous infection and subsequent immunity (IgG). Prenatal diagnosis of fetal varicella infection can be performed using ultrasound at 5 weeks following primary maternal infection. A PCR test of the mother's amniotic fluid can also be performed, though the risk of spontaneous abortion due to the amniocentesis procedure is higher than the risk of the baby developing fetal varicella syndrome.

Laboratory Findings

Molecular Methods

Polymerase chain reaction (PCR)

  • Confirmation of monkeypox virus (MPXV) infection is based on nucleic acid amplification testing, using real-time or conventional PCR, for detection of unique sequences of viral DNA. PCR can be used alone, or in combination with sequencing.
  • PCR kits detecting MPXV are under development but no commercial validated PCR kits are currently available widely.

DNA extraction

  • DNA can be extracted from samples using any standard extraction protocols or kits.
    • Sample lysis in DNA extraction inactivates live virus. Therefore, sample lysis should be performed under a biosafety cabinet.
    • For crust samples, DNA extraction kit for tissue samples should be used to insure appropriate sample lysis.

Molecular detection

  • In case a of monkeypox suspected case, swabs from lesions, crusts and vesicular fluids to be obtained and tested for MPXV using a real-time PCR. Positive findings should be reported to healthcare authorities, followed by distinction of clades: Congo Basin and West African.
  • Some protocols advise to detect OPXV via real-time PCR. Upon positive findings, additional testing for MPXV via real-time PCR to be performed. Confirmed cases of MPXV to be reported to healthcare authorities.


References