Isosporiasis laboratory findings: Difference between revisions
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==Laboratory | ==Laboratory Findings== | ||
Microscopic demonstration of the large, typically shaped | Microscopic demonstration of the large, typically shaped [[oocyst]]s, is the basis for diagnosis. Because the oocysts may be passed in small amounts and intermittently, repeated stool examinations and concentration procedures are recommended. | ||
If stool examinations are negative, examination of duodenal specimens by biopsy or string test (Enterotest®) may be needed. | If stool examinations are negative, examination of duodenal specimens by [[biopsy]] or string test (Enterotest®) may be needed. | ||
The oocysts can be visualized on wet mounts by microscopy with bright-field, differential interference contrast (DIC), and UV fluorescence. They can also be stained by modified acid-fast stain. | The oocysts can be visualized on wet mounts by microscopy with bright-field, differential interference contrast (DIC), and UV fluorescence. They can also be stained by modified [[acid-fast stain]]. | ||
[[Image:Oocysts of Isospora belli.jpg|left|Oocysts of Isospora belli]] | [[Image:Oocysts of Isospora belli.jpg|left|Oocysts of Isospora belli]] | ||
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'''A''', '''B''', '''C''': Oocysts of Isospora belli. The oocysts are large (25 to 30 µm) and have a typical ellipsoidal shape. When excreted, they are immature and contain one sporoblast ('''A''', '''B'''). The oocyst matures after excretion: the single sporoblast divides in two sporoblasts ('''C'''), which develop cyst walls, becoming sporocysts, which eventually contain four sporozoites each. Images contributed by Georgia Division of Public Health. | '''A''', '''B''', '''C''': Oocysts of [[Isospora belli]]. The oocysts are large (25 to 30 µm) and have a typical ellipsoidal shape. When excreted, they are immature and contain one sporoblast ('''A''', '''B'''). The oocyst matures after excretion: the single sporoblast divides in two sporoblasts ('''C'''), which develop cyst walls, becoming sporocysts, which eventually contain four sporozoites each. Images contributed by Georgia Division of Public Health. | ||
[[Image:Comparison coccidian.jpg|left|Comparison of coccidian parasites]] | [[Image:Comparison coccidian.jpg|left|Comparison of coccidian parasites]] | ||
Revision as of 22:14, 12 December 2012
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
Laboratory Findings
Microscopic demonstration of the large, typically shaped oocysts, is the basis for diagnosis. Because the oocysts may be passed in small amounts and intermittently, repeated stool examinations and concentration procedures are recommended. If stool examinations are negative, examination of duodenal specimens by biopsy or string test (Enterotest®) may be needed. The oocysts can be visualized on wet mounts by microscopy with bright-field, differential interference contrast (DIC), and UV fluorescence. They can also be stained by modified acid-fast stain.
A, B, C: Oocysts of Isospora belli. The oocysts are large (25 to 30 µm) and have a typical ellipsoidal shape. When excreted, they are immature and contain one sporoblast (A, B). The oocyst matures after excretion: the single sporoblast divides in two sporoblasts (C), which develop cyst walls, becoming sporocysts, which eventually contain four sporozoites each. Images contributed by Georgia Division of Public Health.
Oocysts of Isospora belli can also be stained with acid-fast stains, and can be visualized by UV fluorescence on wet mounts, as illustrated in Figure 2. Three coccidian parasites that most commonly infect humans, seen in acid-fast stained smears (2A, 2C, 2F), bright-field differential interference contrast (2B, 2D, 2G) and UV fluorescence (2E, 2H, C. parvum oocysts do not autofluoresce).