Enterovirus 68 laboratory findings: Difference between revisions
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===PCR=== | ===PCR=== | ||
*Enterovirus 68 is diagnosed by reverse transcription | *Enterovirus 68 is diagnosed by [[reverse transcription polymerase chain reaction]] ([[RT-PCR]]) testing. | ||
*PCR assay is conducted targeting the 5'-nontranslated region, followed by partial sequencing of the structural protein genes, VP4-VP2, VP1, or both. This gives definitive enterovirus type-specific information. <ref name="MMWR">{{cite web| url=http://www.cdc.gov/mmwr/preview/mmwrhtml/mm6038a1.htm| title=Clusters of Acute Respiratory Illness Associated with Human Enterovirus 68 --- Asia, Europe, and United States, 2008--2010}}</ref> | *[[PCR]] assay is conducted targeting the 5'-nontranslated region, followed by partial sequencing of the structural protein genes, VP4-VP2, VP1, or both. This gives definitive enterovirus type-specific information. <ref name="MMWR">{{cite web| url=http://www.cdc.gov/mmwr/preview/mmwrhtml/mm6038a1.htm| title=Clusters of Acute Respiratory Illness Associated with Human Enterovirus 68 --- Asia, Europe, and United States, 2008--2010}}</ref> | ||
==References== | ==References== | ||
Revision as of 15:20, 8 September 2014
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]
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Overview
Laboratory Findings
- Routine tests such as a complete blood count may not show any abnormalities.
- Leukocytosis with an increased number of lymphocytes can be observed among some patients. [1]
- Infection with non-polio enteroviruses can be confirmed by:[2]
- Isolating or identifying the virus in cell culture
- Polymerase chain reaction (PCR) assay.
- Non-polio enteroviruses can be detected in stool or rectal swabs and respiratory specimens (including from the throat). Depending on the symptoms other specimen types, such as cerebrospinal fluid, blister fluid, and blood, can be collected for testing.
- A positive laboratory test for non-polio enteroviruses from certain specimens, such as rectal or respiratory swab, does not necessarily mean the virus is the cause of infection. Non-polio enteroviruses can be shed for an extended period of time after the symptoms have resolved.
PCR
- Enterovirus 68 is diagnosed by reverse transcription polymerase chain reaction (RT-PCR) testing.
- PCR assay is conducted targeting the 5'-nontranslated region, followed by partial sequencing of the structural protein genes, VP4-VP2, VP1, or both. This gives definitive enterovirus type-specific information. [3]
References
- ↑ Lu, Q.-B.; Wo, Y.; Wang, H.-Y.; Wei, M.-T.; Zhang, L.; Yang, H.; Liu, E.-M.; Li, T.-Y.; Zhao, Z.-T.; Liu, W.; Cao, W.-C. (2013). "Detection of enterovirus 68 as one of the commonest types of enterovirus found in patients with acute respiratory tract infection in China". Journal of Medical Microbiology. 63 (Pt_3): 408–414. doi:10.1099/jmm.0.068247-0. ISSN 0022-2615.
- ↑ "CDC Non-Polio Enterovirus Laboratory Testing".
- ↑ "Clusters of Acute Respiratory Illness Associated with Human Enterovirus 68 --- Asia, Europe, and United States, 2008--2010".