Rotavirus infection laboratory findings: Difference between revisions

Jump to navigation Jump to search
m (Bot: Removing from Primary care)
 
(4 intermediate revisions by 3 users not shown)
Line 4: Line 4:


==Overview==  
==Overview==  
Rotavirus infection diagnosis is approachable through several diagnostic techniques. These techniques include electron microscopy, reverse transcriptase polymerase chain reaction and RNA electrophoresis. Stool examination for the virus is important to detect the extracted viruses. Also, ELISA and immunochromatographic assays can be used for antigen detection.  
Rotavirus infection diagnosis is approachable through several diagnostic techniques. These techniques include [[electron microscopy]], [[Reverse transcription polymerase chain reaction|reverse transcriptase polymerase chain reaction]] and [[RNA]] [[electrophoresis]]. [[Stool examination]] for the virus is important to detect the extracted viruses. Also, [[Enzyme linked immunosorbent assay (ELISA)|ELISA]] and immunochromatographic assays can be used for [[antigen]] detection.


==Laboratory Findings==
==Laboratory Findings==
*Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).<ref name="pmid24379214">{{cite journal| author=Parashar UD, Nelson EA, Kang G| title=Diagnosis, management, and prevention of rotavirus gastroenteritis in children. | journal=BMJ | year= 2013 | volume= 347 | issue=  | pages= f7204 | pmid=24379214 | doi=10.1136/bmj.f7204 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=24379214  }} </ref>
*Specific diagnosis of the [[disease]] is made by identification of the [[virus]] in the patient's [[Stools|stool]]. The most widely used diagnostic laboratory method is [[antigen]] detection in the stool by an [[enzyme immunoassay]] (EIA) directed at an [[antigen]] common to all group A rotaviruses (i.e., those that are the principal cause of human disease).<ref name="pmid24379214">{{cite journal| author=Parashar UD, Nelson EA, Kang G| title=Diagnosis, management, and prevention of rotavirus gastroenteritis in children. | journal=BMJ | year= 2013 | volume= 347 | issue=  | pages= f7204 | pmid=24379214 | doi=10.1136/bmj.f7204 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=24379214  }} </ref>
*Other diagnostic techniques that can be used:  
*Other diagnostic techniques that can be used:  
**Electron microscopy  
**[[Electron microscopy]]
**RNA electrophoresis
**[[RNA]] [[electrophoresis]]
**Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
**Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
**Tissue culture
**Tissue culture


*The virus is usually excreted in the stool in the first phases of the infection so, stool examination is required to detect the virus particles. Polyacrylamid gel electrophoresis can be used to detect the virus in the patients stool.  
*The [[virus]] is usually excreted in the [[stool]] in the first phases of the [[infection]] so, [[stool examination]] is required to detect the [[virus]] particles. [[Polyacrylamide gel]] [[electrophoresis]] can be used to detect the [[virus]] in the patients [[stool]].  


*Antigen detecting tests can be used to diagnose the rotavirus infection. It includes:  
*[[Antigen]] detecting tests can be used to diagnose the [[rotavirus infection]]. It includes:  
**Enzyme Linked Immunosorbent assay (ELISA)
**[[Enzyme linked immunosorbent assay (ELISA)|Enzyme Linked Immunosorbent Assay (ELISA)]]
**Immunochromatographic assay
**Immunochromatographic assay


Line 23: Line 23:
{{reflist|2}}
{{reflist|2}}


[[Category:Disease]]
[[Category:Emergency mdicine]]
[[Category:Up-To-Date]]
[[Category:Infectious disease]]
[[Category:Infectious disease]]
[[Category:Disease]]
[[Category:Gastroenterology]]
[[Category:Pediatrics]]

Latest revision as of 00:03, 30 July 2020

Rotavirus infection Microchapters

Home

Patient Information

Overview

Historical Perspective

Classification

Pathophysiology

Causes

Differentiating Rotavirus infection from other Diseases

Epidemiology and Demographics

Risk Factors

Screening

Natural History, Complications and Prognosis

Diagnosis

History and Symptoms

Physical Examination

Laboratory Findings

Xray

CT scan

MRI

Ultrasound

Other Imaging Findings

Other Diagnostic Studies

Treatment

Medical Therapy

Surgery

Primary Prevention

Secondary Prevention

Future or Investigational Therapies

Case Studies

Case #1

Rotavirus infection laboratory findings On the Web

Most recent articles

Most cited articles

Review articles

CME Programs

Powerpoint slides

Images

American Roentgen Ray Society Images of Rotavirus infection laboratory findings

All Images
X-rays
Echo & Ultrasound
CT Images
MRI

Ongoing Trials at Clinical Trials.gov

US National Guidelines Clearinghouse

NICE Guidance

FDA on Rotavirus infection laboratory findings

CDC on Rotavirus infection laboratory findings

Rotavirus infection laboratory findings in the news

Blogs on Rotavirus infection laboratory findings

Directions to Hospitals Treating Rotavirus infection

Risk calculators and risk factors for Rotavirus infection laboratory findings

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]Associate Editor(s)-in-Chief: Ahmed Elsaiey, MBBCH [2]

Overview

Rotavirus infection diagnosis is approachable through several diagnostic techniques. These techniques include electron microscopy, reverse transcriptase polymerase chain reaction and RNA electrophoresis. Stool examination for the virus is important to detect the extracted viruses. Also, ELISA and immunochromatographic assays can be used for antigen detection.

Laboratory Findings

  • Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).[1]
  • Other diagnostic techniques that can be used:

References

  1. Parashar UD, Nelson EA, Kang G (2013). "Diagnosis, management, and prevention of rotavirus gastroenteritis in children". BMJ. 347: f7204. doi:10.1136/bmj.f7204. PMID 24379214.