Prolamin box gene transcriptions

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Editor-In-Chief: Henry A. Hoff

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A field of maize (corn) is shown. Credit: Löwe 48.{{free media}}

"The BPBF [barley prolamin-box (P-box) binding factor] expressed in bacteria as a GST-fusion binds a P-box 5′-TGTAAAG-3′ containing oligonucleotide derived from the promoter region of anHor2gene."[1]

"The primary structure of hordein [barley prolamins] polypeptides is closely related to that of prolamins from other grass species from the Pooideae subfamily, such as wheat and rye (Shewry & Tatham 1990;Shewry et al. 1995)."[1]

Consensus sequences

"The close evolutionary relationship is also manifested by the conservation of a putative regulatory element in their gene promoters, the endosperm box (Forde et al. 1985;Kreis et al. 1985). This conserved region consists of two motifs, a 7 bp element (5′TGTAAAG3′) termed the Prolamin Box (P-box) or endosperm motif (EM) followed at a distance of up to 8 nucleotides by the GCN4-like motif (GLM) which has the 5′(G/A)TGA(G/C)TCA(T/C)3′ consensus sequence (reviewed by Müller et al. 1995)."[1]

"The main cis-element present in their promoters is an endosperm-specific box [19,20], which consists of two motifs: a GLM (GCN4-like motif) (5′ G(A)TGA(G) GTCAT 3′) that shares homology with yeast GCN4 [21], and a 7 bp P-box (Prolamin box) (5′TGTAAAG3′) [22–24]."[2]

A1BG sampling

For the Basic programs (starting with SuccessablesProl.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand in the negative direction (from ZSCAN22 to A1BG) is SuccessablesProl1--.bas, looking for 3'-TG(A/T)AAAG-5', 1, 3'-TGTAAAG-5', 2884,
  2. negative strand in the positive direction (from ZNF497 to A1BG) is SuccessablesProl1-+.bas, looking for 3'-TG(A/T)AAAG-5', 1, 3'-TGAAAAG-5', 489,
  3. positive strand in the negative direction is SuccessablesProl1+-.bas, looking for 3'-TG(A/T)AAAG-5', 1, 3'-TGAAAAG-5', 1627,
  4. positive strand in the positive direction is SuccessablesProl1++.bas, looking for 3'-TG(A/T)AAAG-5', 0,
  5. complement, negative strand, negative direction is SuccessablesProl1c--.bas, looking for 3'-AC(A/T)TTTC-5', 1, 3'-ACTTTTC-5', 1627,
  6. complement, negative strand, positive direction is SuccessablesProl1c-+.bas, looking for 3'-AC(A/T)TTTC-5', 0,
  7. complement, positive strand, negative direction is SuccessablesProl1c+-.bas, looking for 3'-AC(A/T)TTTC-5', 1, 3'-ACATTTC-5', 2884,
  8. complement, positive strand, positive direction is SuccessablesProl1c++.bas, looking for 3'-AC(A/T)TTTC-5', 1, 3'-ACTTTTC-5', 489,
  9. inverse complement, negative strand, negative direction is SuccessablesProl1ci--.bas, looking for 3'-CTTT(A/T)CA-5', 0,
  10. inverse complement, negative strand, positive direction is SuccessablesProl1ci-+.bas, looking for 3'-CTTT(A/T)CA-5', 0,
  11. inverse complement, positive strand, negative direction is SuccessablesProl1ci+-.bas, looking for 3'-CTTT(A/T)CA-5', 0,
  12. inverse complement, positive strand, positive direction is SuccessablesProl1ci++.bas, looking for 3'-CTTT(A/T)CA-5', 0,
  13. inverse, negative strand, negative direction, is SuccessablesProl1i--.bas, looking for 3'-GAAA(A/T)GT-5', 0,
  14. inverse, negative strand, positive direction, is SuccessablesProl1i-+.bas, looking for 3'-GAAA(A/T)GT-5', 0,
  15. inverse, positive strand, negative direction, is SuccessablesProl1i+-.bas, looking for 3'-GAAA(A/T)GT-5', 0,
  16. inverse, positive strand, positive direction, is SuccessablesProl1i++.bas, looking for 3'-GAAA(A/T)GT-5', 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

Initial content for this page in some instances came from Wikiversity.

See also

References

  1. 1.0 1.1 1.2 Montaña Mena, Jesus Vicente-Carbajosa, Robert J. Schmidt and Pilar Carbonero (1998). "An endosperm-specific DOF protein from barley, highly conserved in wheat, binds to and activates transcription from the prolamin-box of a native B-hordein promoter in barley endosperm". The Plant Journal. 16 (1): 53–62. doi:10.1046/j.1365-313x.1998.00275.x. Retrieved 2017-02-19. Unknown parameter |month= ignored (help)
  2. Veronica Ruta, Chiara Longo, Andrea Lepri, Veronica De Angelis, Sara Occhigrossi, Paolo Costantino and Paola Vittorioso (8 February 2020). "The DOF Transcription Factors in Seed and Seedling Development". Plants. 9 (2): 218. doi:10.3390/plants9020218. Retrieved 7 January 2021.

External links

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