Taxadiene synthase

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In enzymology, a taxadiene synthase (EC 4.2.3.17) is an enzyme that catalyzes the chemical reaction

geranylgeranyl diphosphate taxa-4,11-diene + diphosphate

Hence, this enzyme has one substrate, geranylgeranyl diphosphate, and two products, taxa-4,11-diene and diphosphate.

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is geranylgeranyl-diphosphate diphosphate-lyase (cyclizing, taxa-4,11-diene-forming). Other names in common use include geranylgeranyl-diphosphate diphosphate-lyase (cyclizing,, and taxadiene-forming). This enzyme participates in diterpenoid biosynthesis.

References

  • IUBMB entry for 4.2.3.17
  • BRENDA references for 4.2.3.17 (Recommended.)
  • PubMed references for 4.2.3.17
  • PubMed Central references for 4.2.3.17
  • Google Scholar references for 4.2.3.17
  • Croteau R (1995). "Cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene is the committed step of taxol biosynthesis in Pacific yew". J. Biol. Chem. 270: 8686&ndash, 90. PMID 7721772.
  • Hezari M, Lewis NG, Croteau R (1995). "Purification and characterization of taxa-4(5),11(12)-diene synthase from Pacific yew (Taxus brevifolia) that catalyzes the first committed step of taxol biosynthesis". Arch. Biochem. Biophys. 322: 437&ndash, 44. PMID 7574719.
  • Lin X, Hezari M, Koepp AE, Floss HG, Croteau R (1996). "Mechanism of taxadiene synthase, a diterpene cyclase that catalyzes the first step of taxol biosynthesis in Pacific yew". Biochemistry. 35: 2968&ndash, 77. PMID 8608134.
  • Hezari M, Ketchum RE, Gibson DM, Croteau R (1997). "Taxol production and taxadiene synthase activity in Taxus canadensis cell suspension cultures". Arch. Biochem. Biophys. 337: 185&ndash, 90. PMID 9016812.
  • Coates RM, Williams RM, Croteau R (2000). "Intramolecular proton transfer in the cyclization of geranylgeranyl diphosphate to the taxadiene precursor of taxol catalyzed by recombinant taxadiene synthase". Chem. Biol. 7: 969&ndash, 77. PMID 11137819.

External links

The CAS registry number for this enzyme class is 169277-52-5.

Gene Ontology (GO) codes

Template:4.2-enzyme-stub



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