Polynucleotide 5'-phosphatase

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In enzymology, a polynucleotide 5'-phosphatase (EC is an enzyme that catalyzes the chemical reaction

a 5'-phosphopolynucleotide + H2O a polynucleotide + phosphate

Thus, the two substrates of this enzyme are 5'-phosphopolynucleotide and H2O, whereas its two products are polynucleotide and phosphate.

This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is polynucleotide 5'-phosphohydrolase. This enzyme is also called 5'-polynucleotidase.

Structural studies

As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1D8H, 1D8I, 1I9S, 1I9T, and 1YN9.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
  • PubMed references for
  • PubMed Central references for
  • Google Scholar references for
  • Becker A, Hurwitz J (1967). "The enzymatic cleavage of phosphate termini from polynucleotides". J. Biol. Chem. 242: 936&ndash, 50. PMID 4289819.

External links

The CAS registry number for this enzyme class is 37288-17-8.

Gene Ontology (GO) codes