Phi-X174 phage

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Template:Taxobox group ii entryTemplate:Taxobox familia entryTemplate:Taxobox genus entryTemplate:Taxobox species entryTemplate:Taxobox end placementTemplate:Taxobox end The Phi-X174 phage was the first organism to have its DNA-based genome sequenced by Fred Sanger and his team in 1977[1]. In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of Bacteriophage PhiX-174 DNA[2]. This phage has a very small amount of DNA. It has 11 genes in 5386 bases (it is single stranded) in a circular topology. Several of them expressing similar function in two groups. The GC content is 44% and 95% of nucleotides are coding genes.

See also

References

  1. Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M., Nucleotide sequence of bacteriophage phi X174 DNA, Nature. 1977 Feb 24;265(5596):687-95
  2. Fiers, W., and R. L. Sinsheimer, The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure, J. Mol. Biol. 5:424-434, 1962

External links


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