Methylenetetrahydrofolate dehydrogenase (NADP+)

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In enzymology, a methylenetetrahydrofolate dehydrogenase (NADP+) (EC is an enzyme that catalyzes the chemical reaction

5,10-methylenetetrahydrofolate + NADP+ 5,10-methenyltetrahydrofolate + NADPH + H+

Thus, the two substrates of this enzyme are 5,10-methylenetetrahydrofolate and NADP+, whereas its 3 products are 5,10-methenyltetrahydrofolate, NADPH, and H+.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:NADP+ oxidoreductase. Other names in common use include N5,N10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate:NADP oxidoreductase, 5,10-methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase, and methylenetetrahydrofolate dehydrogenase (NADP). This enzyme participates in glyoxylate and dicarboxylate metabolism and one carbon pool by folate.

Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1A4I, 1DIA, 1DIB, 1DIG, 1LU9, 1LUA, 2C2X, and 2C2Y.


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  • HATEFI Y, OSBORN MJ, KAY LD, HUENNEKENS FM (1957). "Hydroxymethyl tetrahydrofolic dehydrogenase". J. Biol. Chem. 227: 637&ndash, 47. PMID 13462986.
  • OSBORN MJ, HUENNEKENS FM (1957). "Participation of anhydroleucovorin in the hydroxymethyl tetrahydrofolic dehydrogenase system". Biochim. Biophys. Acta. 26: 646&ndash, 7. PMID 13499428.
  • RAMASASTRI BV, BLAKLEY RL (1962). "5,10-Methylenetetrahydrofolic dehydrogenase from bakers' yeast. I Partial purification and some properties". J. Biol. Chem. 237: 1982&ndash, 8. PMID 14490085.
  • Yeh YC, Greenberg DM (1965). "Purification and properties of N5, N10-Methylenetetra-hydrofolate dehydrogenase of calf thymus". Biochim. Biophys. Acta. 105: 279&ndash, 91. PMID 4379024.

External links

The CAS registry number for this enzyme class is 9029-14-5.

Gene Ontology (GO) codes