Lowry protein assay
The Lowry protein assay, or Lowry Method, is a biochemical test to measure the amount of protein in a sample.
Copper(II) ion in alkaline solution reacts with protein to form complexes, which react with the Folin-phenol reagent, a mixture of phosphotungstic acid and phosphomolybdic acid in phenol. The product becomes reduced to molybdenum/tungsten blue and can be detected colorimetrically by absorbance at 750 nm. The higher the concentration of protein, the darker the solution.
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