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Invertase (EC ) (systematic name: beta-fructofuranosidase) is a sucrase enzyme. It catalyzes the hydrolysis (breakdown) of sucrose (table sugar) to fructose and glucose, usually in the form of inverted sugar syrup.

For industrial use, invertase is usually derived from yeast. It is also synthesized by bees, who use it to make honey from nectar.

Invertase is expensive, so when fructose is required, it may be preferable to make it from glucose using glucose isomerase.

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