D-loop

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In molecular biology, a displacement loop or D-loop is a DNA structure where the two strands of a double-stranded DNA molecule are separated for a stretch and held apart by a third strand of DNA. The third strand has a base sequence which is complementary to one of the main strands and pairs with it, thus displacing the other main strand in the region. Within that region the structure is thus a form of triple-stranded DNA. A diagram in the paper introducing the term illustrated the D-loop with a shape resembling a capital "D", where the displaced strand formed the loop of the "D".[1]

(An unrelated use of the term D-loop, but not displacement loop, is for the loop of RNA that forms the end of the D arm of a transfer RNA molecule.)

D-loops occur in a number of particular situations, including in DNA repair, in telomeres, and as a semi-stable structure in mitochondrial circular DNA molecules.

In mitochondria

Researchers at Cal Tech discovered in 1971 that the circular mitochondrial DNA from growing cells included a short segment of three strands which they called a displacement loop.[1] They found the third strand was a replicated segment of the heavy strand (or H-strand) of the molecule, which it displaced, and was hydrogen bonded to the light strand (or L-strand). Since then, it has been shown that the third strand is the initial segment generated by a replication of the heavy strand that has been arrested shortly after initiation and is often maintained for some period in that state.[2] The D-loop occurs in the main non-coding area of the mitochondrial DNA molecule, a segment called the control region or D-loop region.

Replication of the mitochondrial DNA can occur in two different ways, both starting in the D-loop region.[3] One way continues replication of the heavy strand through a substantial part (e.g. two-thirds) of the circular molecule, and then replication of the light strand begins. The more recently reported mode starts at a different origin within the D-loop region and uses coupled-strand replication with simultaneous synthesis of both strands.[3][4]

Certain bases within the D-loop region are conserved, but large parts are highly variable and the region has proven to be useful for the study of the evolutionary history of vertebrates.[5] The region contains promoters for the transcription of RNA from the two strands of mitochondrial DNA immediately adjacent to the D-loop structure that is associated with initiation of DNA replication.[6]

The function of the D-loop which occurs in mitochondrial DNA is not yet clear, but recent research suggests that it participates in the organization of the mitochondrial nucleoid.[7][8]

In telomeres

In 1999 it was reported that telomeres, which cap the end of chromosomes, terminate in a lariat-like structure termed a t-loop.[9] This is a loop of both strands of the chromosome which are joined to an earlier point in the double-stranded DNA by the 3' strand end invading the strand pair to form a D-loop; a special protein stabilizes the joint. The t-loop which is completed by the D-loop splice protects the end of the chromosome from damage.[10]

In DNA repair

When a double-stranded DNA molecule has suffered a break in both strands, one repair mechanism available in diploid eukaryotic cells is homologous recombination repair. This makes use of the intact chromosome homologous to the broken one as a template to bring the two double-stranded pieces into correct alignment for rejoining. Early in this process, one strand of one piece is matched to a strand of the intact chromosome and that strand is used to form a D-loop at that point, displacing the intact chromosome's other strand. Various ligation and synthesis steps follow to effect the rejoining.[11]

In humans, the protein RAD51 is central to the homologous search and formation of the D-loop. In the bacterium Escherichia coli, a similar function is performed by the protein RecA.[12]

See also

References

  1. 1.0 1.1 Kasamatsu, H et al. (1971), "A novel closed-circular mitochondrial DNA with properties of a replicating intermediate", Proc Natl Acad Sci USA' (1971 Sep) 68(9):2252-7. PMID 5289384
  2. Doda, JN et al. (1981), "Elongation of displacement-loop strands in human and mouse mitochondrial DNA is arrested near specific template sequences", Proc Natl Acad Sci USA (1981 Oct) 78(10):6116-20. PMID 6273850
  3. 3.0 3.1 Fish, J et al. (2004), "Discovery of a major D-loop replication origin reveals two modes of human mtDNA synthesis", Science (2004 Dec 17) 306(5704):2098-101. PMID 15604407
  4. Holt IJ et al. (2000), "Coupled leading- and lagging-strand synthesis of mammalian mitochondrial DNA". Cell 100:515–524. PMID 10721989
  5. Larizza A et al. (2002), "Lineage Specificity of the Evolutionary Dynamics of the mtDNA D-Loop Region in Rodents", J Mol Evol. (2002 Feb) 54(2):145-55. PMID 11821908
  6. Chang, DD et al. (1985), "Priming of human mitochondrial DNA replication occurs at the light-strand promoter", Proc Natl Acad Sci USA (1985 Jan) 82(2):351-5. PMID 2982153
  7. He, Jiuya et al. (2007), "The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization", J Cell Biol. 2007 January 15; 176(2): 141–146. doi:10.1083/jcb.200609158
  8. Leslie, Mitch (2007), "Thrown for a D-loop", J Cell Biol. 2007 January 15; 176(2): 129. doi:10.1083/jcb.1762iti3
  9. Griffith, JD et al. (1999), "Mammalian telomeres end in a large duplex loop", Cell (1999 May 14) 97(4):503-14. PMID 10338214
  10. Greider, CW (1999), "Telomeres do D-loop-T-loop", Cell (1999 May 14) 97(4):419-22. PMID 10338204
  11. Hartl, Daniel L. and Jones, Elizabeth W. (2005), Genetics: Analysis of Genes and Genomes (Jones & Bartlett Publishers), p. 251.
  12. Shibata, T et al. (2001), "Homologous genetic recombination as an intrinsic dynamic property of a DNA structure induced by RecA/Rad51-family proteins: a possible advantage of DNA over RNA as genomic material", Proc Natl Acad Sci USA (2001 Jul 17) 98(15):8425-32. PMID 11459985



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