Ureaplasma urealyticum laboratory findings

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]Associate Editor(s)-in-Chief: Fatimo Biobaku M.B.B.S [2]

Overview

Microbial culture method and/or PCR-based techniques are the two reliable methods for making a diagnosis of Ureaplasma colonization/infection. PCR-based techniques are more sensitive than culture

Laboratory Findings

Colonization and infection with Ureaplasma species are diagnosed based on culture results and/or PCR.[1][2][3][4][5][6][7]

Microscopy

Culture method: Ureaplasma species have been cultured from different sites of the body of infants such as the blood, CSF, nasopharynx, endotracheal secretions, gastric aspirates, pleural fluid, lung and brain tissue. Ureaplasma species are also the most common organism isolated from infected amniotic fluid and placenta. Culture from sites such as the urogenital tract, rectum, and joint aspirate, has been documented. Conventional bacteriologic culture methods cannot identify these species and special culture for Mycoplasma is often used.

Molecular-based test

PCR-based method: This is a rapid and more sensitive technique for detection of Ureaplasma species compared to microbial culture method.

References

  1. Yoneda N, Yoneda S, Niimi H, Ueno T, Hayashi S, Ito M; et al. (2016). "Polymicrobial Amniotic Fluid Infection with Mycoplasma/Ureaplasma and Other Bacteria Induces Severe Intra-Amniotic Inflammation Associated with Poor Perinatal Prognosis in Preterm Labor". Am J Reprod Immunol. 75 (2): 112–25. doi:10.1111/aji.12456. PMID 26668114.
  2. Ollikainen J, Hiekkaniemi H, Korppi M, Katila ML, Heinonen K (1993). "Ureaplasma urealyticum cultured from brain tissue of preterm twins who died of intraventricular hemorrhage". Scand J Infect Dis. 25 (4): 529–31. PMID 8248756.
  3. Ollikainen J, Hiekkaniemi H, Korppi M, Sarkkinen H, Heinonen K (1993). "Ureaplasma urealyticum infection associated with acute respiratory insufficiency and death in premature infants". J Pediatr. 122 (5 Pt 1): 756–60. PMID 8496757.

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