Rox1p gene transcriptions

Associate Editor(s)-in-Chief: Henry A. Hoff

"All promoters recognized by pol-II may require one or more UASs for regulated gene expression [36,37]."^[1]

Human genes

Gene expressions

"Inducible promoters can initiate a dramatic change in the transcriptional level of their corresponding genes upon the presence or absence of specific stimuli. In S. cerevisiae, these stimuli, or inducers, range from carbon sources, including glucose, galactose, sucrose, maltose, glycerol, acetate and ethanol, to environmental factors such as the temperature, pH, stress and light, to others, such as metabolites, amino acids, metal ions and hormones [9,10,11]."^[1]

An inducer for the Rox1 transcription factor is Heme which functions for a Heme-dependent repressor of hypoxic genes.^[1]

Interactions

Consensus sequences

"The upstream activating sequence (UAS) is located upstream of the core promoter and serves as a binding site for specific transcription activators."^[1]

UAS for the Rox1p transcription factor is RRRTAACAAGAG.^[1]

Binding site for

Complement copies

Inverse copies

Complement-inverse copies

Enhancer activity

Promoter occurrences

The UAS for Rox1p occurs in the S. cerevisiae HEM13, COX5B, ANB1, and CYC7 promoters.^[1]

Hypotheses

A1BG has no regulatory elements in either promoter.
A1BG is not transcribed by a regulatory element.
No regulatory element participates in the transcription of A1BG.

Samplings

Copying a responsive elements consensus sequence GGGTAA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence (A/G)(A/G)(A/G)TAACAAGAG (starting with SuccessablesROX.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

negative strand, negative direction, looking for AAAAAAAA, 0.
positive strand, negative direction, looking for AAAAAAAA, 0.
positive strand, positive direction, looking for AAAAAAAA, 0.
negative strand, positive direction, looking for AAAAAAAA, 0.
complement, negative strand, negative direction, looking for TTTTTTTT, 0.
complement, positive strand, negative direction, looking for TTTTTTTT, 0.
complement, positive strand, positive direction, looking for TTTTTTTT, 0.
complement, negative strand, positive direction, looking for TTTTTTTT, 0.
inverse complement, negative strand, negative direction, looking for TTTTTTTT, 0.
inverse complement, positive strand, negative direction, looking for TTTTTTTT, 0.
inverse complement, positive strand, positive direction, looking for TTTTTTTT, 0.
inverse complement, negative strand, positive direction, looking for TTTTTTTT, 0.
inverse negative strand, negative direction, looking for AAAAAAAA, 0.
inverse positive strand, negative direction, looking for AAAAAAAA, 0.
inverse positive strand, positive direction, looking for AAAAAAAA, 0.
inverse negative strand, positive direction, looking for AAAAAAAA, 0.

ROX UTRs

ROX core promoters

ROX proximal promoters

ROX distal promoters

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

References

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

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