https://www.wikidoc.org/index.php?title=Masson%27s_trichrome_stain&feed=atom&action=historyMasson's trichrome stain - Revision history2024-03-28T10:56:22ZRevision history for this page on the wikiMediaWiki 1.40.0https://www.wikidoc.org/index.php?title=Masson%27s_trichrome_stain&diff=679151&oldid=prevWikiBot: Robot: Automated text replacement (-{{SIB}} + & -{{EH}} + & -{{EJ}} + & -{{Editor Help}} + & -{{Editor Join}} +)2012-08-09T17:12:42Z<p>Robot: Automated text replacement (-{{SIB}} + & -{{EH}} + & -{{EJ}} + & -{{Editor Help}} + & -{{Editor Join}} +)</p>
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'''Masson's trichrome''' is a three-color [[staining (biology)|staining]] protocol used in [[histology]]. The recipes evolved from the original Masson's formulation to different specific applications, but all are suited for distinguishing [[cell (biology)|cells]] from surrounding [[connective tissue]].<br />
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Most recipes produce red [[keratin]] and [[muscle fiber]]s, blue or green [[collagen]] and [[bone]], light red or pink [[cytoplasm]], and dark brown to black [[cell nucleus|cell nuclei]].<br />
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The trichrome is applied by immersion of the fixated sample into [[Weigert's iron hematoxylin]], and then three different solutions, labeled A, B, and C:<br />
* Weigert's [[hematoxylin]] is a sequence of three solutions: [[ferric chloride]] in diluted [[hydrochloric acid]], [[hematoxylin]] in 95% [[ethanol]], and [[potassium ferricyanide]] solution alkalized by [[sodium borate]]. It is used to stain the nuclei.<br />
* Solution A, also called '''plasma stain''', contains [[fuchsin|acid fuchsin]], [[Ponceau 2R|Xylidine Ponceau]], [[glacial acetic acid]], and [[distilled water]]. Other red acid dyes can be used, eg. the [[Biebrich scarlet]] in Lillie's trichrome.<br />
* Solution B contains [[phosphomolybdic acid]] in distilled water.<br />
* Solution C, also called '''fibre stain''', contains [[Light Green SF yellowish]], or alternatively [[Fast Green FCF]]. It is used to stain collagen. If blue is preferred to green, [[methyl blue]], [[water blue]] or [[aniline blue]] can be substituted.<br />
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A common variant is [[Lillie's trichrome]]. It is often errorneously called Masson's trichrome. It differs in the dyes used, their concentrations, and the immersion times.<br />
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==External links==<br />
* [http://stainsfile.info/StainsFile/stain/conektv/masson.htm Stainsfile: Masson's Trichrome]<br />
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[[Category:Staining]]<br />
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[[pt:Corante tricromo de Masson]]<br />
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