Eosin: Difference between revisions

Revision as of 19:10, 6 April 2015

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

Eosin is a fluorescent acidic / negative compound that binds to and forms salts with basic, or eosinophilic, compounds containing positive charges (such as proteins that are basic / positive due to the presence of amino acid residues such as Arginine and Lysine) and stains them dark red or pink as a result of the actions of bromine on fluorescein. In addition to staining proteins in the cytoplasm, it can be used to stain collagen and muscle fibers for examination under the microscope. Structures that stain readily with eosin are termed eosinophilic.

Etymology

The name Eosin comes from Eos, the Ancient Greek word for 'dawn' and the name of the Ancient Greek goddess of the dawn.

Variants

There are actually two very closely related compounds commonly referred to as eosin. Most often used is Eosin Y (also known as eosin Y ws, eosin yellowish, Acid Red 87, C.I. 45380, bromoeosine, bromofluoresceic acid, D&C Red No. 22); it has a very slightly yellowish cast. The other eosin compound is eosin B (eosin bluish, Acid Red 91, C.I. 45400, Saffrosine, Eosin Scarlet, or imperial red); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is a matter of preference and tradition.

Eosin Y is a tetrabromo derivative of fluorescein.^[1] Eosin B is a dibromo dinitro derivative of fluorescein.^[2]

Use in histology

File:Histological sample of Trachea obtained at "Instituto Nacional de Cardiología Ignacio A. Chávez".JPG

Sample of a trachea coloured with hematoxylin and eosin.

Eosin is most often used as a counterstain to hematoxylin in H&E (haematoxylin and eosin) staining. H&E staining is one of the most commonly used techniques in histology. Tissue stained with haematoxylin and eosin shows cytoplasm stained pink-orange and nuclei stained darkly, either blue or purple. Eosin also stains red blood cells intensely red.

For staining, eosin Y is typically used in concentrations of 1 to 5 percent weight by volume, dissolved in water or ethanol.^[3] For prevention of mold growth in aqueous solutions, thymol is sometimes added.^[4] A small concentration (0.5 percent) of acetic acid usually gives a deeper red stain to the tissue.

It is listed as an IARC class 3 carcinogen.

References

Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974.

External links

Eosin Y - Applications information

Template:Antiseptics and disinfectants

[1] Template:SMILESCAS

[2] Template:SMILESCAS

[3] ttp://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/

[4] ttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC291425/pdf/aem00234-0146.pdf

[1]

[2]

[3]

[4]

@@ Line 1: / Line 1: @@
-{{SI}}
+__Notoc__
+{{CMG}}
+{{for|the eosin glazing process|Zsolnay}}
+[[Image:Eosin Y.svg|thumb|right|200px|[[Eosin Y]]]]
+[[Image:Eosin B.png|thumb|right|200px|[[Eosin B]]]]
-'''Eosin''' is a [[fluorescent]] red [[dye]] resulting from the action of [[bromine]] on [[fluorescein]]. It can be used to stain [[cytoplasm]], [[collagen]] and [[Muscle#Muscular Composition|muscle fibers]] for examination under the [[microscope]].
+'''Eosin''' is a [[fluorescent]] acidic / negative compound that binds to and forms salts with basic, or eosinophilic, compounds containing positive charges (such as proteins that are basic / positive due to the presence of amino acid residues such as Arginine and Lysine) and stains them dark red or pink as a result of the actions of [[bromine]] on [[fluorescein]]. In addition to staining proteins in the [[cytoplasm]], it can be used to stain [[collagen]] and [[Muscle#Muscular Composition|muscle fibers]] for examination under the [[microscope]].  Structures that stain readily with eosin are termed [[eosinophilic]].
-There are actually two very closely related compounds commonly referred to as eosin.  Most often used is '''eosin Y''' (also known as '''eosin Y ws''', '''eosin yellowish''', '''Acid Red 87''', '''C.I. 45380''', '''bromoeosine''', '''bromofluoresceic acid''', '''D&C Red No. 22'''); it has a very slightly yellowish cast. The other eosin compound is '''eosin B''' ('''eosin bluish''', '''Acid Red 91''', '''C.I. 45400''', '''Saffrosine''', '''Eosin Scarlet''', or '''imperial red'''); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is a matter of preference and tradition.
+==Etymology==
+The name Eosin comes from [[Eos]], the [[Ancient Greek]] word for 'dawn' and the name of the Ancient Greek goddess of the dawn.
-Eosin is most often used as a [[counterstain]] to [[haematoxylin]] in [[H&E stain|H&E (haematoxylin and eosin) staining]].  H&E staining is one of the most commonly used techniques in [[histology]]. [[biological tissue|Tissue]] stained with [[hematoxylin]] and eosin shows [[cytoplasm]] stained pink-orange and [[cell nucleus|nuclei]] stained darkly, either blue or purple. Eosin also stains [[erythrocyte|red blood cells]] intensely red. Eosin is an acidic dye and shows up in the basic parts of the cell, ie the cytoplasm. [[Hematoxylin]] however is a basic dye and shows up in the acidic part of the cell. For example the nucleus, where [[nucleic acids]] ([[DNA]] and [[RNA]]) are concentrated.
-Structures that stain readily with eosin are termed [[eosinophilic]].
-Eosin Y is a tetrabromo derivate of [[fluorescein]]. {{SMILESCAS|CAS=17372-87-1|SMILES=O=C5C(Br)=C2O C1=C(Br)C([O-]) =C(Br)C=C1C(C4=C (C([O-])=O)C=C C=C4)=C2C=C3Br}}
-Eosin B is a dibromo dinitro derivate of [[fluorescein]]. {{SMILESCAS|CAS=548-28-3|SMILES=O=C5C(Br)=C2O C1=C(Br)C([O-]) =C([N+]([O-])=O) C=C1C(C4=C(C([O-]) =O)C=CC=C4)=C2 C=C3[N+]([O-])=O}}
+==Variants==
+There are actually two very closely related compounds commonly referred to as eosin.  Most often used is '''[[Eosin Y]]''' (also known as '''eosin Y ws''', '''eosin yellowish''', '''Acid Red 87''', '''C.I. 45380''', '''bromoeosine''', '''bromofluoresceic acid''', '''D&C Red No. 22'''); it has a very slightly yellowish cast. The other eosin compound is '''[[eosin B]]''' ('''eosin bluish''', '''Acid Red 91''', '''C.I. 45400''', '''Saffrosine''', '''Eosin Scarlet''', or '''imperial red'''); it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is a matter of preference and tradition.
-==Recipes for [[Histology]]==
+[[Eosin Y]] is a tetrabromo derivative of [[fluorescein]].<ref>{{SMILESCAS|CAS=17372-87-1|SMILES=O=C5C(Br)=C2O C1=C(Br)C([O-]) =C(Br)C=C1C(C4=C (C([O-])=O)C=C C=C4)=C2C=C3Br}}</ref> [[Eosin B]] is a dibromo dinitro derivative of [[fluorescein]].<ref>{{SMILESCAS|CAS=548-28-3|SMILES=O=C5C(Br)=C2O C1=C(Br)C([O-]) =C([N+]([O-])=O) C=C1C(C4=C(C([O-]) =O)C=CC=C4)=C2 C=C3[N+]([O-])=O}}</ref>
-==='''5% Aqueous Eosin Y'''===
+==Use in histology==
-'''Formula:'''
+[[File:Histological sample of Trachea obtained at "Instituto Nacional de Cardiología Ignacio A. Chávez".JPG|thumb|Sample of a trachea coloured with [[hematoxylin]] and eosin.]]
-* 5 gm Eosin Y
+Eosin is most often used as a [[counterstain]] to [[hematoxylin]] in [[H&E stain|H&E (haematoxylin and eosin) staining]].  H&E staining is one of the most commonly used techniques in [[histology]]. [[biological tissue|Tissue]] stained with [[haematoxylin]] and eosin shows [[cytoplasm]] stained pink-orange and [[cell nucleus|nuclei]] stained darkly, either blue or purple. Eosin also stains [[erythrocyte|red blood cells]] intensely red.
-* 100 mL Distilled water
-Dissolve Eosin Y crystals in water by gentle heating. Cool and Filter. [[Thymol]] crystals may be added to prevent formation of molds.
+For staining, eosin Y is typically used in concentrations of 1 to 5 percent weight by volume, dissolved in water or [[ethanol]].<ref>http://protocolsonline.com/histology/dyes-and-stains/haematoxylin-eosin-he-staining/</ref> For prevention of mold growth in aqueous solutions, [[thymol]] is sometimes added.<ref>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC291425/pdf/aem00234-0146.pdf</ref> A small concentration (0.5 percent) of [[acetic acid]] usually gives a deeper red stain to the tissue.
-==='''Eosin, Stock Alcoholic Solution'''===
+It is listed as an [[International Agency for Research on Cancer|IARC]] [[List of IARC Group 3 carcinogens|class 3 carcinogen]].
-'''Formula:'''
-* 1 gm Eosin Y
-* 20 mL Distilled water
-* 95% Alcohol
-Dissolve Eosin Y in the water by gentle heating. Cool and add Alcohol.
-For use, one part of the stock solution is usually diluted with three parts of 80% Alcohol. Addition of 0.5 mL Glacial [[Acetic Acid]] (HOAc) for every 100 mL of stain will usually give a deeper red stain to the tissue.
-==Etymology==
-The name Eosin comes from Eos, the Greek word for 'dawn' and the name of the Greek Goddess of the Dawn.[http://www.m-w.com/dictionary/eosin]
 ==See also==
-* [[Hematoxylin]]
+* [[Haematoxylin]]
 * [[H&E stain]]
+* [[Romanowsky stain]]
-==External links==
+* [[Merbromin]]
-*[http://www.abbeycolor.com/eosin-y.php Eosin Y - Applications information]
 ==References==
 *Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974.
-== Acknowledgements ==
+{{reflist}}
-The content on this page was first contributed by: C. Michael Gibson, M.S., M.D.
-Initial content for this page in some instances may have came from [http://www.wikipedia.org Wikipedia] and http://www.askdrwiki.com
+==External links==
+*[http://www.abbeycolor.com/eosin-y.php Eosin Y - Applications information]
-'''List of contributors:'''
-== Suggested Reading and Key General References ==
-== Suggested Links and Web Resources ==
-== For Patients ==
-----
-[[Category:Pathology]]
-[[Category:Histology]]
-[[de:Eosin]]
+{{Antiseptics and disinfectants}}
-[[es:Eosina]]
-[[fr:Éosine]]
-[[it:Eosina]]
-[[hu:Eozin (festék)]]
-[[nl:Eosine]]
-[[pl:Eozyna]]
-[[pt:Eosina]]
-[[ru:Эозин]]
-[[sv:Eosin]]
-[[tr:Eozin]]
-{{WH}}
+[[Category:Fluorone dyes]]
-{{WikiDoc Sources}}
+[[Category:Staining dyes]]
+[[Category:Organobromides]]