Donovanosis laboratory findings: Difference between revisions
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==Laboratory Findings== | ==Laboratory Findings== | ||
===Microscopy=== | |||
*Identification of Donovan bodies in tissue smears has proven sufficient for a strong diagnosis of donovanosis. Donovan bodies are sufficiently unique from other etiologic agents that parasitize macrophages.<ref name="Richens">{{cite journal| author=Richens J| title=The diagnosis and treatment of donovanosis (granuloma inguinale). | journal=Genitourin Med | year= 1991 | volume= 67 | issue= 6 | pages= 441-52 | pmid=1774048 | doi= | pmc=PMC1194766 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=1774048 }} </ref> | |||
*Slides can be created using two methods:<ref name=" O'Farrell">{{cite journal| author=O'Farrell N| title=Donovanosis. | journal=Sex Transm Infect | year= 2002 | volume= 78 | issue= 6 | pages= 452-7 | pmid=12473810 | doi= | pmc=PMC1758360 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12473810 }} </ref> | |||
:*Tissue smear: after cleaning [[ulcer]] surface with saline, a cotton swab is rolled over the ulcer and then rolled over a slide. | |||
:*Tissue biopsy: tissue can be obtained from lesion with punch biopsy, forceps, or scalpel and then crushed between two slides. The lesion is often friable but local anesthetic may be necessary. | |||
*Slides are then stained with Giemsa, Leishman's, or Wright's stain to reveal intracellular Donovan bodies within [[monocytes]] that may or may not be capsulated.<ref name=" O'Farrell"></ref> | |||
:*Haematoloxylin and eosin are poor stains. | |||
==References== | ==References== | ||
Revision as of 16:28, 3 March 2016
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Kalsang Dolma, M.B.B.S.[2]; Nate Michalak, B.A.
Overview
Laboratory Findings
Microscopy
- Identification of Donovan bodies in tissue smears has proven sufficient for a strong diagnosis of donovanosis. Donovan bodies are sufficiently unique from other etiologic agents that parasitize macrophages.[1]
- Slides can be created using two methods:[2]
- Tissue smear: after cleaning ulcer surface with saline, a cotton swab is rolled over the ulcer and then rolled over a slide.
- Tissue biopsy: tissue can be obtained from lesion with punch biopsy, forceps, or scalpel and then crushed between two slides. The lesion is often friable but local anesthetic may be necessary.
- Slides are then stained with Giemsa, Leishman's, or Wright's stain to reveal intracellular Donovan bodies within monocytes that may or may not be capsulated.[2]
- Haematoloxylin and eosin are poor stains.