]> 2026-07-31T09:38:58+00:00 WBR0099 0 en 5'...GGC TAC GTA AAG AAG TCT...3' 5'...GGT ACG TGA AGA AGA TCT...3' This sequence comprises two mutations: One mutation from 5'...GGC T to GGT...3' reflects a one base pair deletion; while the other is a one base pair insertion after 5'...AAG AAG T...3' to 5'...AAG AAG AT...3'. The first mutation disrupts the frame of the sequence whereas the second mutation restores it. The above sequence thus reflects 5 missense mutations with no nonsense mutations. The series of two mutations is extremely unlikely and does not commonly explain the genetic disorder observed in Duchenne muscular dystrophy. 5'...GGC TAA GTG AAG AAG TCT...3' This mutation reflects the insertion of an inappropriate stop codon 5'...TAC to TAA...3'. mRNA transcription of this sequence will be: 5'...GGC UAA GUG AAG AAG UCU...3'. The second trinucleotide UAA is a stop codon, which does not correspond to any amino acid. It leads to the formation of a truncated dystrophin protein. 5'...GGC TAC GTG ATG AAG TCT...3' 5'...GGC TAC GTG AAG AAG AAG...(x20)...TCT...3' Genetics A 8-year-old boy is brought to the pediatrician's office by his mother for chronic weakness. She reports that her child started to have trouble running and rising from chairs at the age of 5. He has become progressively weaker over the past 3 years and now requires braces to walk. Physical examination is remarkable for proximal weakness of the upper and lower extremities. The pediatrician also notes atrophy of the gluteus maximus and accessory muscles of the pelvis, with unusually large calf muscles. The physician suspects a genetic disease and refers the patient to genetic testing. Which of the following mRNA sequences is most likely present in this patient? Normal DNA Sequence: <br>5'...GGC TAC GTG AAG AAG TCT...3' <br>3'...CCG ATG CAC TTC TTC AGA...5' C Musculoskeletal/Rheumatology 2020-10-27T23:20:38Z 2459150.472662 WBR0099