Nucleoplasm: Difference between revisions
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The subcellular distribution of a substance to or within the nucleus is often referred to as '''nuclear localization'''.<ref name=Nieva>{{ cite journal |author=Nieva C, Gwoźdź T, Dutko-Gwoźdź J, Wiedenmann J, Spindler-Barth M, Wieczorek E, Dobrucki J, Duś D, Henrich V, Ożyhar A, Spindler KD |title=Ultraspiracle promotes the nuclear localization of ecdysteroid receptor in mammalian cells |year=2005 |journal=Biol Chem. |volume=386 |issue=5 |pages=463-70 |doi=10.1515/BC.2005.055 }}</ref> | The subcellular distribution of a substance to or within the nucleus is often referred to as '''nuclear localization'''.<ref name=Nieva>{{ cite journal |author=Nieva C, Gwoźdź T, Dutko-Gwoźdź J, Wiedenmann J, Spindler-Barth M, Wieczorek E, Dobrucki J, Duś D, Henrich V, Ożyhar A, Spindler KD |title=Ultraspiracle promotes the nuclear localization of ecdysteroid receptor in mammalian cells |year=2005 |journal=Biol Chem. |volume=386 |issue=5 |pages=463-70 |doi=10.1515/BC.2005.055 }}</ref> | ||
[[NPM1|Nucleophosmin]] has been found to require ATP and/or CTP for translocation (taking approximately 2-4 hrs) from nucleoli into the nucleoplasm as verified by immunofluorescent localization.<ref name=Wu>{{ cite journal |author=Wu MH, Lam CY, Yung BY |title=Translocation of nucleophosmin from nucleoli to nucleoplasm requires ATP |journal=Biochem J. |year=1995 |month=Feb |volume=305 |issue=Pt 3 |pages=987-92 |pmid=1136355 }}</ref> The redistribution of nucleophosmin into the nucleoplasm was considered uniform, occurs during [[interphase]] | [[NPM1|Nucleophosmin]] has been found to require ATP and/or CTP for translocation (taking approximately 2-4 hrs) from nucleoli into the nucleoplasm as verified by immunofluorescent localization.<ref name=Wu>{{ cite journal |author=Wu MH, Lam CY, Yung BY |title=Translocation of nucleophosmin from nucleoli to nucleoplasm requires ATP |journal=Biochem J. |year=1995 |month=Feb |volume=305 |issue=Pt 3 |pages=987-92 |pmid=1136355 }}</ref> The redistribution of nucleophosmin into the nucleoplasm was considered uniform, occurs during [[interphase]] or [[Cellular differentiation|differentiation]], and is a normal cellular process.<ref name=Wu/> | ||
=Binding to the nucleoplasm= | =Binding to the nucleoplasm= |
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Editor-In-Chief: Henry A. Hoff
Overview
Similar to the cytoplasm of a cell, the nucleus contains nucleoplasm or nuclear sap. The nucleoplasm is one of the types of protoplasm, and it is often enveloped by the nuclear membrane or nuclear envelope. The nucleoplasm is a highly viscous liquid that surrounds the chromosomes and nucleoli. Many substances such as nucleotides (necessary for purposes such as the replication of DNA) and enzymes (which direct activities that take place in the nucleus) are dissolved in the nucleoplasm. A network of fibers known as the nuclear matrix can also be found in the nucleoplasm. The nucleoplasm is partly made up of nucleohyaloplasm and nucleosol. The nucleoplasm is also colorless.
Introduction
A plasm is a formative or formed material; i.e., something molded. Usually a formed material keeps it shape once the mold is removed. Should something be glassy or transparent it can be said to be hyaloid. Whether a fluid is molded or liquid is often a matter of viscosity and whether a fluid is transparent, translucent, or opaque is often a matter of absorption or scattering.
Visibility of the nucleoplasm
Factors that can increase the visibility of the nucleoplasm include the state of lateral aggregation of deoxyribonucleic acid and the presence of fibrils.[1] In E. coli, for example, the nucleoplasm has a formed shape that can be altered by fixation.[1]
Nuclear localization
The subcellular distribution of a substance to or within the nucleus is often referred to as nuclear localization.[2]
Nucleophosmin has been found to require ATP and/or CTP for translocation (taking approximately 2-4 hrs) from nucleoli into the nucleoplasm as verified by immunofluorescent localization.[3] The redistribution of nucleophosmin into the nucleoplasm was considered uniform, occurs during interphase or differentiation, and is a normal cellular process.[3]
Binding to the nucleoplasm
As a molded material, the nucleoplasm can serve as a target for binding molecules. For example, thyroid hormones have been found to bind to it.[4]
References
- ↑ 1.0 1.1 Woldringh CL, Nanninga N (1976). "Organization of the nucleoplasm in Escherichia coli visualized by phase-contrast light microscopy, freeze fracturing, and thin sectioning". J Bacteriol. 127 (3): 1455–64. PMID 232941. Unknown parameter
|month=
ignored (help) - ↑ Nieva C, Gwoźdź T, Dutko-Gwoźdź J, Wiedenmann J, Spindler-Barth M, Wieczorek E, Dobrucki J, Duś D, Henrich V, Ożyhar A, Spindler KD (2005). "Ultraspiracle promotes the nuclear localization of ecdysteroid receptor in mammalian cells". Biol Chem. 386 (5): 463–70. doi:10.1515/BC.2005.055.
- ↑ 3.0 3.1 Wu MH, Lam CY, Yung BY (1995). "Translocation of nucleophosmin from nucleoli to nucleoplasm requires ATP". Biochem J. 305 (Pt 3): 987–92. PMID 1136355. Unknown parameter
|month=
ignored (help) - ↑ Adylova AT, Turakulov IaKh (1997). "Specific binding of triiodothyronine to the nucleoplasm". Biull Eksp Biol Med. 123 (2): 190–2. doi:10.1007/BF02766449. PMID 9280501. Unknown parameter
|month=
ignored (help)
bg:Кариоплазма
cs:Karyoplazma
de:Karyoplasma
it:Nucleoplasma
sr:Нуклеоплазма