Rotavirus infection laboratory findings: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
|||
| Line 8: | Line 8: | ||
==Laboratory Findings== | ==Laboratory Findings== | ||
*Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).<ref name="pmid24379214">{{cite journal| author=Parashar UD, Nelson EA, Kang G| title=Diagnosis, management, and prevention of rotavirus gastroenteritis in children. | journal=BMJ | year= 2013 | volume= 347 | issue= | pages= f7204 | pmid=24379214 | doi=10.1136/bmj.f7204 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=24379214 }} </ref> | *Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).<ref name="pmid24379214">{{cite journal| author=Parashar UD, Nelson EA, Kang G| title=Diagnosis, management, and prevention of rotavirus gastroenteritis in children. | journal=BMJ | year= 2013 | volume= 347 | issue= | pages= f7204 | pmid=24379214 | doi=10.1136/bmj.f7204 | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=24379214 }} </ref> | ||
Other diagnostic techniques that can be used: | *Other diagnostic techniques that can be used: | ||
**Electron microscopy | **Electron microscopy | ||
**RNA electrophoresis | **RNA electrophoresis | ||
Revision as of 14:52, 14 May 2017
|
Rotavirus infection Microchapters |
|
Diagnosis |
|---|
|
Treatment |
|
Case Studies |
|
Rotavirus infection laboratory findings On the Web |
|
American Roentgen Ray Society Images of Rotavirus infection laboratory findings |
|
Risk calculators and risk factors for Rotavirus infection laboratory findings |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]Associate Editor(s)-in-Chief: Ahmed Elsaiey, MBBCH [2]
Overview
Rotavirus infection diagnosis is approachable through several diagnostic techniques. These techniques include electron microscopy, reverse transcriptase polymerase chain reaction and RNA electrophoresis. Stool examination for the virus is important to detect the extracted viruses. Also, ELISA and immunochromatographic assays can be used for antigen detection.
Laboratory Findings
- Specific diagnosis of the disease is made by identification of the virus in the patient's stool. The most widely used diagnostic laboratory method is antigen detection in the stool by an enzyme immunoassay (EIA) directed at an antigen common to all group A rotaviruses (i.e., those that are the principal cause of human disease).[1]
- Other diagnostic techniques that can be used:
- Electron microscopy
- RNA electrophoresis
- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
- Tissue culture
- The virus is usually excreted in the stool in the first phases of the infection so, stool examination is required to detect the virus particles. Polyacrylamid gel electrophoresis can be used to detect the virus in the patients stool.
- Antigen detecting tests can be used to diagnose the rotavirus infection. It includes:
- Enzyme Linked Immunosorbent assay (ELISA)
- Immunochromatographic assay
References
- ↑ Parashar UD, Nelson EA, Kang G (2013). "Diagnosis, management, and prevention of rotavirus gastroenteritis in children". BMJ. 347: f7204. doi:10.1136/bmj.f7204. PMID 24379214.