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==Overview==
 
There are no pathognomonic laboratory findings for Chikungunya virus infection. Laboratory abnormalities include mild [[thrombocytopenia]] (>100,000/mm<sup>3</sup>), [[neutropenia]], [[lymphopenia]], and elevated [[liver function test]]s. [[ESR|Erythrocyte sedimentation rate]] and [[CRP|C-reactive protein]] are usually elevated.<ref name=PAHO2011>{{cite book | last = | first = | title = Preparedness and response for Chikungunya virus introduction in the Americas | publisher = Pan American Health Organization CDC, Center for Disease Control and Prevention | location = Washington, DC | year = 2011 | isbn = 978-92-75-11632-6 }}</ref>


==Laboratory Findings==
==Laboratory Findings==
Common laboratory tests for chikungunya include [[RT-PCR]], virus isolation, and serological tests.


*Virus isolation provides the most definitive diagnosis, but takes one to two weeks for completion and must be carried out in [[biosafety level 3]] laboratories.<ref name="WHO diagnosis">{{cite web |url=http://www.searo.who.int/EN/Section10/Section2246_12902.htm |title=WHO&nbsp;— Laboratory Diagnosis of Chikungunya Fevers |work= |accessdate=2008-07-11}}</ref> The technique involves exposing specific [[cell line]]s to samples from whole blood and identifying chikungunya virus-specific responses.
Laboratory abnormalities include the followings:<ref name=PAHO2011>{{cite book | last = | first = | title = Preparedness and response for Chikungunya virus introduction in the Americas | publisher = Pan American Health Organization CDC, Center for Disease Control and Prevention | location = Washington, DC | year = 2011 | isbn = 978-92-75-11632-6 }}</ref>


*RT-PCR using [[nested primer]] pairs is used to amplify several chikungunya-specific [[gene]]s from whole blood. Results can be determined in one to two days.
* Mild [[thrombocytopenia]] (>100,000/mm<sup>3</sup>)
* [[Leukopenia]] (including [[neutropenia]] and [[lymphopenia]])
* Elevated [[AST]] and/or [[ALT]]
* Elevated [[ESR|erythrocyte sedimentation rate (ESR)]]  
* Elevated [[CRP|C-reactive protein (CRP)]]
 
==References==


*Serological diagnosis requires a larger amount of blood than the other methods, and uses an [[ELISA]] assay to measure chikungunya-specific [[IgM]] levels. Results require two to three days, and [[false positive]]s can occur with infection via other related viruses, such as [[o'nyong'nyong virus]] and [[Semliki Forest virus]].
{{reflist|2}}


==External Links==


==References==
* [http://www.cdc.gov/Chikungunya/index.html CDC Chikungunya virus]
{{Reflist|2}}


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Latest revision as of 17:23, 18 September 2017

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Alejandro Lemor, M.D. [2], Alonso Alvarado, M.D. [3]

Overview

There are no pathognomonic laboratory findings for Chikungunya virus infection. Laboratory abnormalities include mild thrombocytopenia (>100,000/mm3), neutropenia, lymphopenia, and elevated liver function tests. Erythrocyte sedimentation rate and C-reactive protein are usually elevated.[1]

Laboratory Findings

Laboratory abnormalities include the followings:[1]

References

  1. 1.0 1.1 Preparedness and response for Chikungunya virus introduction in the Americas. Washington, DC: Pan American Health Organization CDC, Center for Disease Control and Prevention. 2011. ISBN 978-92-75-11632-6.

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