H&E stain

(Redirected from Hematoxylin and eosin stain)
Jump to navigation Jump to search

WikiDoc Resources for H&E stain

Articles

Most recent articles on H&E stain

Most cited articles on H&E stain

Review articles on H&E stain

Articles on H&E stain in N Eng J Med, Lancet, BMJ

Media

Powerpoint slides on H&E stain

Images of H&E stain

Photos of H&E stain

Podcasts & MP3s on H&E stain

Videos on H&E stain

Evidence Based Medicine

Cochrane Collaboration on H&E stain

Bandolier on H&E stain

TRIP on H&E stain

Clinical Trials

Ongoing Trials on H&E stain at Clinical Trials.gov

Trial results on H&E stain

Clinical Trials on H&E stain at Google

Guidelines / Policies / Govt

US National Guidelines Clearinghouse on H&E stain

NICE Guidance on H&E stain

NHS PRODIGY Guidance

FDA on H&E stain

CDC on H&E stain

Books

Books on H&E stain

News

H&E stain in the news

Be alerted to news on H&E stain

News trends on H&E stain

Commentary

Blogs on H&E stain

Definitions

Definitions of H&E stain

Patient Resources / Community

Patient resources on H&E stain

Discussion groups on H&E stain

Patient Handouts on H&E stain

Directions to Hospitals Treating H&E stain

Risk calculators and risk factors for H&E stain

Healthcare Provider Resources

Symptoms of H&E stain

Causes & Risk Factors for H&E stain

Diagnostic studies for H&E stain

Treatment of H&E stain

Continuing Medical Education (CME)

CME Programs on H&E stain

International

H&E stain en Espanol

H&E stain en Francais

Business

H&E stain in the Marketplace

Patents on H&E stain

Experimental / Informatics

List of terms related to H&E stain


H&E stained lung tissue sample from an end-stage emphysema patient. RBCs are red, nuclei are blue-purple, and other cellular and extracellular material is pink.

H&E stain, or hematoxylin and eosin stain, is a popular staining method in histology. It is the most widely used stain in medical diagnosis; for example when a pathologist looks at a biopsy of a suspected cancer, the histological section is likely to be stained with H&E and termed H&E section, H+E section, or HE section.

The staining method involves application of the basic dye hematoxylin, which colors basophilic structures with blue-purple hue, and alcohol-based acidic eosin Y, which colors eosinophilic structures bright pink.

The basophilic structures are usually the ones containing nucleic acids, such as the ribosomes and the chromatin-rich cell nucleus, and the cytoplasmatic regions rich in RNA.

The eosinophilic structures are generally composed of intracellular or extracellular protein. The Lewy bodies and Mallory bodies are examples of eosinophilic structures. Most of the cytoplasm is eosinophilic. Red blood cells are stained intensely red.

The structures do not have to be acidic or basic to be called basophilic and eosinophilic. The terminology is based on the affinity to the dyes.

Other colors, e.g. yellow and brown, can be present in the sample; they are caused by intrinsic pigments, e.g. melanin.

Some structures do not stain well. Basal laminae need to be stained by PAS stain or some silver stains, if they have to be well visible. Reticular fibers also require silver stain. Hydrophobic structures also tend to remain clear; these are usually rich in fats, eg. adipocytes, myelin around neuron axons, and Golgi apparatus membranes.

See also

External Links


it:Colorazione con ematossilina eosina


Template:WikiDoc Sources