** In addition to its well-known [[Immunosuppression|immunosuppressive]] capabilities, the one-time administration of [[cyclosporine]] at the time of percutaneous coronary intervention (PCI) has been found to deliver a 40 percent reduction in [[infarct]] size in a small group proof of concept study of human patients with [[reperfusion injury]] published in The New England Journal of Medicine in 2008.[[File:Myocardial Treatment In IRI.jpg|thumb|Treatment OF IRI]][[Cyclosporine]] has been confirmed in studies to inhibit the actions of [[Cyclophilin|cyclophilin D]], a protein which is induced by excessive [[Intracellular calcium-sensing proteins|intracellular calcium]] flow to interact with other pore components and help open the [[Mitochondrial membrane transport protein|MPT pore]]. Inhibiting [[Cyclophilin|cyclophilin D]] has been shown to prevent the opening of the MPT pore and protect the [[Mitochondrion|mitochondria]] and cellular energy production from excessive [[calcium]] inflows.
** In addition to its well-known [[Immunosuppression|immunosuppressive]] capabilities, the one-time administration of [[cyclosporine]] at the time of [[percutaneous coronary intervention]] (PCI) has been found to deliver a 40 percent reduction in [[infarct]] size in a small group proof of concept study of human patients with [[reperfusion injury]] published in The New England Journal of Medicine in 2008.[[File:Myocardial Treatment In IRI.jpg|thumb|Treatment OF IRI]][[Cyclosporine]] has been confirmed in studies to inhibit the actions of [[Cyclophilin|cyclophilin D]], a protein which is induced by excessive [[Intracellular calcium-sensing proteins|intracellular calcium]] flow to interact with other pore components and help open the [[Mitochondrial membrane transport protein|MPT pore]]. Inhibiting [[Cyclophilin|cyclophilin D]] has been shown to prevent the opening of the MPT pore and protect the [[Mitochondrion|mitochondria]] and cellular energy production from excessive [[calcium]] inflows.
**[[Reperfusion]] leads to biochemical imbalances within the cell that lead to [[cell death]] and increased [[Infarct|infarct size]]. More specifically, [[calcium]] overload and excessive production of [[Reactive oxygen species|reactive oxygen]] species in the first few minutes after [[reperfusion]] set off a cascade of biochemical changes that result in the opening of the so-called [[Mitochondrial permeability transition|mitochondrial permeability transition pore]] (MPT pore) in the [[mitochondrial membrane]] of [[Heart|cardiac cells]].
**[[Reperfusion]] leads to biochemical imbalances within the cell that lead to [[cell death]] and increased [[Infarct|infarct size]]. More specifically, [[calcium]] overload and excessive production of [[Reactive oxygen species|reactive oxygen]] species in the first few minutes after [[reperfusion]] set off a cascade of biochemical changes that result in the opening of the so-called [[Mitochondrial permeability transition|mitochondrial permeability transition pore]] (MPT pore) in the [[mitochondrial membrane]] of [[Heart|cardiac cells]].
** The opening of the MPT pore leads to the inrush of water into the [[mitochondria]], resulting in mitochondrial dysfunction and collapse. Upon collapse, the [[calcium]] is then released to overwhelm the next mitochondria in a cascading series of events that cause [[Mitochondrion|mitochondria]]<nowiki/>l energy production supporting the cell to be reduced or stopped completely. The cessation of [[energy]] production results in [[cellular death]]. Protecting mitochondria is a viable [[cardio]] protective strategy.
**<nowiki/>The opening of the MPT pore leads to the inrush of water into the [[mitochondria]], resulting in [[Mitochondrion|mitochondrial]] dysfunction and [[Collapse (medical)|collapse]]. Upon collapse, the [[calcium]] is then released to overwhelm the next [[Mitochondrion|mitochondria]] in a cascading series of events that cause [[Mitochondrion|mitochondria]]<nowiki/>l energy production supporting the cell to be reduced or stopped completely. The cessation of [[energy]] production results in [[cellular death]]. Protecting [[Mitochondrion|mitochondria]] is a viable [[cardio]] protective strategy.
**[[Cyclosporine]] is currently in a phase II/III (adaptive) clinical study in Europe to determine its ability to [[ameliorate]] [[neuronal]] cellular damage in traumatic [[brain injury]].
**[[Cyclosporine]] is currently in a phase II/III (adaptive) clinical study in Europe to determine its ability to [[ameliorate]] [[neuronal]] cellular damage in traumatic [[brain injury]].
*'''TRO40303'''
*'''TRO40303'''
** TRO40303 is a new [[cardio]] protective compound that was shown to inhibit the [[Mitochondrial membrane transport protein|MPT pore]] and reduce infarct size after [[ischemia]]-[[reperfusion]].
** TRO40303 is a new [[cardio]] protective compound that was shown to inhibit the [[Mitochondrial membrane transport protein|MP]]<nowiki/>[[Mitochondrial membrane transport protein|T pore]] and reduce [[infarct]] size after [[ischemia]]-[[reperfusion]].
*'''Stem cell therapy'''
*'''Stem cell therapy'''
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*'''Superoxide dismutase'''
*'''Superoxide dismutase'''
** Superoxide dismutase is an important antioxidant enzyme that transforms superoxide anions into water and hydrogen peroxide. Recent work has demonstrated important therapeutic effects on pre-clinical models of reperfusion damage following an ischemic stroke.
**[[Superoxide dismutase]] is an important [[antioxidant]] enzyme that transforms [[superoxide]] [[anions]] into water and [[hydrogen peroxide]]. Recent work has demonstrated important therapeutic effects on pre-clinical models of [[reperfusion]] damage following an [[ischemic stroke]].
*'''Metformin'''
*'''Metformin'''
** A series of 2009 studies published in the Journal of Cardiovascular Pharmacology indicate that metformin may prevent injury to cardiac reperfusion by inhibiting Mitochondrial Complex I and opening up MPT pore and in rats.
** A series of 2009 studies published in the Journal of [[Cardiovascular]] Pharmacology indicate that [[metformin]] may prevent injury to [[cardiac]] [[reperfusion]] by inhibiting Mitochondrial Complex I and opening up MPT pore and in rats.
*'''Cannabinoids'''
*'''Cannabinoids'''
** A research published in 2012 shows that the synthetic analog of phytocannabinoid tetrahydrocannabivarin (THCV), 8-Tetrahydrocannabivarin (THCV) and its 11-OH-8-THCV metabolite prevents hepatic ischemia/reperfusion injury by minimizing oxidative stress and inflammatory reactions through cannabinoid CB2 receptors, thereby lowering tissue damage and protective effects of inflammation. Pretreatment with a CB2 receptor antagonist, whereas a CB1 antagonist appeared to strengthen it, attenuated the defensive effects of somewhere else.
** A research published in 2012 shows that the synthetic analog of phytocannabinoid [[tetrahydrocannabivarin]] (THCV), 8-Tetrahydrocannabivarin (THCV) and its 11-OH-8-THCV metabolite prevents [[hepatic]] ischemia/reperfusion injury by minimizing [[oxidative stress]] and [[inflammatory]] reactions through cannabinoid CB2 receptors, thereby lowering [[tissue]] damage and protective effects of [[inflammation]]. Pretreatment with a [[Cannabinoid receptor|CB2]] receptor antagonist, whereas a [[CB1]] antagonist appeared to strengthen it, attenuated the defensive effects of somewhere else.
** An earlier study published in 2011 found that cannabidiol (CBD) also protects against hepatic ischemia/reperfusion injury by attenuating inflammatory signals and oxidative and nitrative stress response, resulting in cell death and tissue damage, but is independent of classic CB1 and CB2 receptors.
** An earlier study published in 2011 found that [[cannabidiol]] (CBD) also protects against [[hepatic]] ischemia/reperfusion injury by attenuating [[inflammatory]] signals and [[oxidative]] and nitrative stress response, resulting in [[cell]] death and [[tissue]] damage, but is independent of classic [[Cannabinoid receptor type 1|CB1]] and [[Cannabinoid receptor|CB]]2 receptors.
==Therapies Associated with Improved Clinical Outcomes==
==Therapies Associated with Improved Clinical Outcomes==
There are several preliminary studies in mice that seem to show that treatment with hydrogen sulfide ( H2S) could have a protective effect against reperfusion injury..
Cyclosporine
In addition to its well-known immunosuppressive capabilities, the one-time administration of cyclosporine at the time of percutaneous coronary intervention (PCI) has been found to deliver a 40 percent reduction in infarct size in a small group proof of concept study of human patients with reperfusion injury published in The New England Journal of Medicine in 2008.Treatment OF IRICyclosporine has been confirmed in studies to inhibit the actions of cyclophilin D, a protein which is induced by excessive intracellular calcium flow to interact with other pore components and help open the MPT pore. Inhibiting cyclophilin D has been shown to prevent the opening of the MPT pore and protect the mitochondria and cellular energy production from excessive calcium inflows.
The opening of the MPT pore leads to the inrush of water into the mitochondria, resulting in mitochondrial dysfunction and collapse. Upon collapse, the calcium is then released to overwhelm the next mitochondria in a cascading series of events that cause mitochondrial energy production supporting the cell to be reduced or stopped completely. The cessation of energy production results in cellular death. Protecting mitochondria is a viable cardio protective strategy.
Cyclosporine is currently in a phase II/III (adaptive) clinical study in Europe to determine its ability to ameliorateneuronal cellular damage in traumatic brain injury.
A series of 2009 studies published in the Journal of Cardiovascular Pharmacology indicate that metformin may prevent injury to cardiacreperfusion by inhibiting Mitochondrial Complex I and opening up MPT pore and in rats.
Cannabinoids
A research published in 2012 shows that the synthetic analog of phytocannabinoid tetrahydrocannabivarin (THCV), 8-Tetrahydrocannabivarin (THCV) and its 11-OH-8-THCV metabolite prevents hepatic ischemia/reperfusion injury by minimizing oxidative stress and inflammatory reactions through cannabinoid CB2 receptors, thereby lowering tissue damage and protective effects of inflammation. Pretreatment with a CB2 receptor antagonist, whereas a CB1 antagonist appeared to strengthen it, attenuated the defensive effects of somewhere else.
An earlier study published in 2011 found that cannabidiol (CBD) also protects against hepatic ischemia/reperfusion injury by attenuating inflammatory signals and oxidative and nitrative stress response, resulting in cell death and tissue damage, but is independent of classic CB1 and CB2 receptors.
Therapies Associated with Improved Clinical Outcomes
Medical treatment in IRI
Therapies that have been associated with improved clinical outcomes include:
Post conditioning (short repeated periods of vessel opening by repeatedly blowing the balloon up for short periods of time).
Mechanisms of protection include formation and release of several autacoids and cytokines, maintained acidosis during early repercussion, activation of protein kinases, and attenuation of opening of the mitochondrial permeability transition pore (MPTP)
One study in humans demonstrated an area under the curve (AUC) of creatine kinase (C) release over the first 3 days of reperfusion (as a surrogate for infarct size) was significantly reduced by 36% in the postconditioned versus control group
Infarct size reduction by PCI postconditioning persisted 6 months after AMI and resulted in a significant improvement in left ventricular (LV) function at 1 year
Inhibition of mitochondrial pore opening by cyclosporine.
Specifically, the study by Piot et al demonstrated that administration of cyclosporine at the time of reperfusion was associated with a reduction in infarct size
Infarct size was measured by the release of creatine kinase and delayed hyperenhancement on MRI
Patients with cardiac arrest, ventricular fibrillation, cardiogenic shock, stent thrombosis, previous acute myocardial infarction, or angina within 48 hours before infarction were not included in the study #*Occlusion of the culprit artery (TIMI flow 0) was part of the inclusion criteria.
Limitations to applying strategies that have demonstrated benefit in animal models is the fact that reperfusion therapy was administered prior to or at the time of reperfusion. In the management of STEMI patients, it is impossible to administer the agent before vessel occlusion (except during coronary artery bypass grafting). Given the time constraints and the goal of opening an occluded artery within 90 minutes, it is also difficult to administer experimental agents before reperfusion in STEMI.
Therapies Associated with Limited Success
Pharmacotherapies that have either failed or that have met with limited success in improving clinical outcomes include:
GIK (glucose-insulin-potassium infusion) (Studied in the Glucose-Insulin-Potassium Infusion in Patients With Acute Myocardial Infarction Without Signs of Heart Failure: The Glucose-Insulin-Potassium Study (GIPS)-II and other older studies
Sodium-hydrogen exchange inhibitors such as cariporide (Studied in the GUARDIAN and EXPIDITION trials)
Adenosine (Studied in the AMISTAD I and AMISTAD II trials as well as the ATTACC trial ). It should be noted that at high doses in anterior ST elevation MIs, adenosine was effective in the AMISTAD trial. Likewise, intracoronary administration of adenosine prior to primary PCI has been associated with improved echocardiographic and clinical outcomes in one small study.
Antibodies directed against leukocyte adhesion molecules such as CD 18 (Studied in the LIMIT AMI trial )
Oxygen free radical scavengers/anti-oxidants, including Erythropoietin, estrogen, heme-oxygenase 1, and hypoxia induced factor-1 (HIF-1).
Pexelizumab, a humanized monoclonal antibody that binds the C5 component of complement (Studied in the Pexelizumab for Acute ST-Elevation Myocardial Infarction in Patients Undergoing Primary Percutaneous Coronary Intervention (APEX AMI) trial )
KAI-9803, a delta-protein kinase C inhibitor (Studied in the Intracoronary KAI-9803 as an adjunct to primary percutaneous coronary intervention for acute ST-segment elevation myocardial infarction trial or DELTA AMI trial).
Human atrial natriuretic peptide (Studied in the Human atrial natriuretic peptide and nicorandil as adjuncts to reperfusion treatment for acute myocardial infarction (J-WIND): two randomized trials.)
FX06, an anti-inflammatory fibrin derivative that competes with fibrin fragments for binding with the vascular endothelial molecule VE-cadherin which deters migration of leukocytes across the endothelial cell monolayer (studied in the F.I.R.E. trial (Efficacy of FX06 in the Prevention of Myocardial Reperfusion Injury)
Magnesium, which was evaluated by the Fourth International Study of Infarct Survival (ISIS-4) and the MAGIC trial.
Hyperoxemia, the delivery of supersaturated oxygen after PCI (Studied in the AMIHOT II trial).
Bendavia studied in the EMBRACE STEMI trial
There are several explanations for why trials of experimental agents have failed in this area:
The therapy was administered after reperfusion and after reperfusion injury had set in
The greatest benefit is observed in anterior ST elevation myocardial infarctions (as demonstrated in the AMISTAD study), and inclusion of non anterior locations minimizes the potential benefit
There are uninhibited redundant pathways mediating reperfusion injury
