RFC5

Replication factor C (activator 1) 5, 36.5kDa
Identifiers
Symbols	RFC5 ; MGC1155; RFC36
External IDs	Template:OMIM5 HomoloGene: 6730
Gene ontology Molecular function Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Cellular component Template:GNF GO Template:GNF GO Biological process Template:GNF GO Template:GNF GO
RNA expression pattern
File:PBB GE RFC5 203209 at tn.png
File:PBB GE RFC5 203210 s at tn.png
File:PBB GE RFC5 213734 at tn.png
More reference expression data
Orthologs
Template:GNF Ortholog box
Species	Human	Mouse
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a

Replication factor C (activator 1) 5, 36.5kDa, also known as RFC5, is a human gene.

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Further reading

• Okumura K, Nogami M, Taguchi H; et al. (1995). "Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23". Genomics. 25 (1): 274–8. PMID 7774928.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• O'Donnell M, Onrust R, Dean FB; et al. (1993). "Homology in accessory proteins of replicative polymerases--E. coli to humans". Nucleic Acids Res. 21 (1): 1–3. PMID 8441605.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Uhlmann F, Cai J, Flores-Rozas H; et al. (1996). "In vitro reconstitution of human replication factor C from its five subunits". Proc. Natl. Acad. Sci. U.S.A. 93 (13): 6521–6. PMID 8692848.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Mossi R, Jónsson ZO, Allen BL; et al. (1997). "Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen". J. Biol. Chem. 272 (3): 1769–76. PMID 8999859.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Cujec TP, Cho H, Maldonado E; et al. (1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme". Mol. Cell. Biol. 17 (4): 1817–23. PMID 9121429.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Cai J, Gibbs E, Uhlmann F; et al. (1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme". J. Biol. Chem. 272 (30): 18974–81. PMID 9228079.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Loor G, Zhang SJ, Zhang P; et al. (1998). "Identification of DNA replication and cell cycle proteins that interact with PCNA". Nucleic Acids Res. 25 (24): 5041–6. PMID 9396813.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Ellison V, Stillman B (1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity". J. Biol. Chem. 273 (10): 5979–87. PMID 9488738.
• Cai J, Yao N, Gibbs E; et al. (1998). "ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits". Proc. Natl. Acad. Sci. U.S.A. 95 (20): 11607–12. PMID 9751713.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Zhang G, Gibbs E, Kelman Z; et al. (1999). "Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen". Proc. Natl. Acad. Sci. U.S.A. 96 (5): 1869–74. PMID 10051561.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A (2001). "Purification and characterization of human DNA damage checkpoint Rad complexes". Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11236–41. doi:10.1073/pnas.201373498. PMID 11572977.CS1 maint: Multiple names: authors list (link)
• Griffith JD, Lindsey-Boltz LA, Sancar A (2002). "Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy". J. Biol. Chem. 277 (18): 15233–6. doi:10.1074/jbc.C200129200. PMID 11907025.CS1 maint: Multiple names: authors list (link)
• Ohta S, Shiomi Y, Sugimoto K; et al. (2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Maruyama T, Farina A, Dey A; et al. (2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Mol. Cell. Biol. 22 (18): 6509–20. PMID 12192049.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Venclovas C, Colvin ME, Thelen MP (2003). "Molecular modeling-based analysis of interactions in the RFC-dependent clamp-loading process". Protein Sci. 11 (10): 2403–16. PMID 12237462.CS1 maint: Multiple names: authors list (link)
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Merkle CJ, Karnitz LM, Henry-Sánchez JT, Chen J (2003). "Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment". J. Biol. Chem. 278 (32): 30051–6. doi:10.1074/jbc.M211591200. PMID 12766176.CS1 maint: Multiple names: authors list (link)
• Bermudez VP, Maniwa Y, Tappin I; et al. (2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA". Proc. Natl. Acad. Sci. U.S.A. 100 (18): 10237–42. doi:10.1073/pnas.1434308100. PMID 12930902.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Zou L, Liu D, Elledge SJ (2004). "Replication protein A-mediated recruitment and activation of Rad17 complexes". Proc. Natl. Acad. Sci. U.S.A. 100 (24): 13827–32. doi:10.1073/pnas.2336100100. PMID 14605214.CS1 maint: Multiple names: authors list (link)
• Ota T, Suzuki Y, Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)