View source for MALSU1

{{Infobox_gene}}
'''MALSU1''' is a [[gene]] on [[chromosome 7]] in humans that encodes the [[protein]] '''MALSU1'''.<ref name="entrez">{{cite web | title = Entrez Protein: LOC_115416| url =https://www.ncbi.nlm.nih.gov/protein/19923977| accessdate = }}</ref>  This protein localizes to mitochondria and is probably involved in mitochondrial translation or the [[biogenesis]] of the large subunit of the [[mitochondrial ribosome]].

== Protein ==

'''MALSU1''' is a member of the DUF143<ref name="DUF143">{{cite web | url =https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=119897| title =  DUF 143 |date = | format = | work = |publisher = | pages = | language = | archiveurl = | archivedate = | quote = | accessdate =}}</ref> family (= [[domain of unknown function]] [https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=119897 143], {{Pfam|PF02410}}) which is highly conserved in both [[prokaryote]]s and [[eukaryotes]] but not [[archaea]].<ref name="urlBLINK">{{cite web | url = https://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=19923977&amp;ordinalpos=1&amp;itool=EntrezSystem2.PEntrez.Sequence.Sequence_SingleReportPanel.Sequence_SingleReportDisplayBar | title =  Pre-computed BLAST results for LOC115416 [Homo sapiens] | date = | format = | work = BLINK ("BLAST Link") | publisher = National Center for Biotechnology Information, United States National Institutes of Health | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = }}</ref>  Examples of mammalian conservation are given below using the ALIGN tool from the [[San Diego Supercomputer Center]] Biology Workbench.<ref name="SDSC Bioworkbench">{{cite web | url = http://workbench.sdsc.edu/ | title =  SDSC Biology Workbench | date = | format = | work =  | publisher =  | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = }}</ref>  Percentages indicate the identity shared by the human protein and the respective mammalian protein.  Accession numbers are from the NCBI database.<br />
{| class="wikitable" border="1"
|-
! Species
! Accession #
! Identity
|-
| ''[[Macaca mulatta]]''
| XP_001098609
| 93.20%
|-
| ''[[Sus scrofa]]''
| NP_001092054
| 85.50%
|-
| ''[[Bos taurus]]''
| NP_001068866
| 88.50%
|-
| ''[[Canis familiaris]]''
| XP_853850
| 77.30%
|-
| ''[[Rattus norvegicus]]''
| NP_001100063
| 81.60%
|-
| [[Equus caballus]]
| XP_001497879
| 90.70%
|}

There are no known or predicted paralogs in ''[[Homo sapiens]]''. That is, MALSU1 is a single-copy gene.

The domain is from position 93 to 194 on the human protein and comprises 43.2% of the sequence.  This conserved domain is also present in the plant gene '''[[iojap]]''', a pattern-striping gene in maize.<ref name="iojap definition">{{cite web | title = InterPro: IPR004394 Iojap-related protein|url =http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004394| accessdate = }}</ref><ref name="CDD Conserved Domain Database">{{cite journal |vauthors=Marchler-Bauer A, Anderson JB, Derbyshire MK, DeWeese-Scott C, Gonzales NR, Gwadz M, Hao L, He S, Hurwitz DI, Jackson JD, Ke Z, Krylov D, Lanczycki CJ, Liebert CA, Liu C, Lu F, Lu S, Marchler GH, Mullokandov M, Song JS, Thanki N, Yamashita RA, Yin JJ, Zhang D, Bryant SH | title = CDD: a conserved domain database for interactive domain family analysis. |journal = Nucleic Acid Residues| volume = 35 | issue = D237-40 | pages = D237–40|date=November 2006 | pmid = 17135202 | doi = 10.1093/nar/gkl951| url = | issn = | pmc = 1751546 }}</ref> However, since its function has been solved at least in bacteria, it is no longer a "domain of unknown function".

===Protein function===
[[File:RsfS mechanism.png|thumb|Mechanism of ribosomal subunit dissociation by RsfS (= RsfA)]]While the function of the protein in mitochondria is not conclusive<ref name="urlMitoProt">{{cite web | url = http://ihg2.helmholtz-muenchen.de/cgi-bin/paolo/mitofilter?seq=MGPGGRVARLLAPLMWRRAVSSVAGSAVGAEPGLRLLAVQRLPVGAAFCRACQTPNFVRGLHSEPGLEER%0D%0AAEGTVNEGRPESDAADHTGPKFDIDMMVSLLRQENARDICVIQVPPEMRYTDYFVIVSGTSTRHLHAMAF%0D%0AYVVKMYKHLKCKRDPHVKIEGKDTDDWLCVDFGSMVIHLMLPETREIYELEKLWTLRSYDDQLAQIAPET%0D%0AVPEDFILGIEDDTSSVTPVELKCE%0D%0A&seqname= | title =  MitoProt II - v1.101 | date = | format = | work = MitoProt II - v1.101 prediction | publisher = Institut für Humangenetik, Technische Universität, München | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = }}</ref><ref name="pmid8944766">{{cite journal |vauthors=Claros MG, Vincens P | title = Computational method to predict mitochondrially imported proteins and their targeting sequences | journal = European Journal of Biochemistry / FEBS | volume = 241 | issue = 3 | pages = 779–86 |date=November 1996 | pmid = 8944766 | doi = 10.1111/j.1432-1033.1996.00779.x | url = | issn = }}</ref> its bacterial homolog has been shown to silence [[prokaryotic translation]] by blocking the two [[ribosomal]] [[Protein subunit|subunit]]s from joining, hence it was called '''RsfS''' (= ribosomal silencing factor in starvation or stationary phase, a synonym of RsfA<ref name="rsfs">{{Cite journal 
| last1 = Häuser | first1 = R. 
| last2 = Pech | first2 = M. 
| last3 = Kijek | first3 = J. 
| last4 = Yamamoto | first4 = H. 
| last5 = Titz | first5 = B. R. 
| last6 = Naeve | first6 = F. 
| last7 = Tovchigrechko | first7 = A. 
| last8 = Yamamoto | first8 = K. 
| last9 = Szaflarski | first9 = W. 
| last10 = Takeuchi | first10 = N. 
| last11 = Stellberger | first11 = T. 
| last12 = Diefenbacher | first12 = M. E. 
| last13 = Nierhaus | first13 = K. H. 
| last14 = Uetz | first14 = P. 
| editor1-last = Hughes 
| editor1-first = Diarmaid 
| title = RsfA (YbeB) Proteins Are Conserved Ribosomal Silencing Factors 
| doi = 10.1371/journal.pgen.1002815 
| journal = PLoS Genetics 
| volume = 8 
| issue = 7 
| pages = e1002815 
| year = 2012 
| pmid = 22829778 
| pmc =3400551 
}}</ref>).

'''Protein-protein interactions'''. RsfS has been shown via a yeast two-hybrid screen to interact with ribosomal protein L14 in four bacterial species as well as in mitochondria.<ref name=rsfs/> MALSU1 was shown to interact with CHMP protein<ref name="pmid16730941">{{cite journal |vauthors=Tsang HT, Connell JW, Brown SE, Thompson A, Reid E, Sanderson CM | title = A systematic analysis of human CHMP protein interactions: additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex | journal = Genomics | volume = 88 | issue = 3 | pages = 333–46 |date=September 2006 | pmid = 16730941 | doi = 10.1016/j.ygeno.2006.04.003 | url = | issn = }}</ref> which is part of the [[ESCRT-III]] complex (Endosomal Sorting Complex Required for Transport). DUF143 has also been shown to interact with UFD1, tRNA synthetases class II, and Cytidylyltransferase in various architectures.<ref name="Pfam">{{cite web | title = Sanger Institute Domain Organization ''PF02410'' | url =http://pfam.sanger.ac.uk/family?acc=PF02410| accessdate = }}</ref>

=== Properties ===
<!-- Image with unknown copyright status removed: [[image:DUF143 structural representation.png|thumb|left|A graphical representation of DUF143. {{deletable image-caption}}]] -->
[[Bioinformatics]] predicted the following properties for LOC_115416:
* '''Molecular Weight''':  26.2 KDal
* '''Isoelectric Point''':  5.155

== Gene ==

'''C7ORF30''' is located on chromosome 7 in humans and runs from 23,305,465 to 23,315,705.<ref name="Gene Loc">{{cite web | title = UCSD Genome Browser| url =http://genome.ucsc.edu/cgi-bin/hgTracks?position=chr7:23305465-23315705&db=hg18&ss=../trash/hgSs/hgSs_genome_7cc7_76f200.pslx+../trash/hgSs/hgSs_genome_7cc7_76f200.fa&hgsid=132001649| accessdate = }}</ref>  There are four predicted exons in the human gene with conservation occurring across most mammalian species.  There is no conclusive data regarding whether the gene is ubiquitously expressed in human tissues, but [[expressed sequence tag]] databases show that it is expressed in many tissues.<ref name="Unigene">{{cite web | title = NCBI Unigene Expression Data| url =https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?UGID=141058&TAXID=9606&SEARCH=| accessdate = }}</ref>

=== Neighboring Genes ===

'''MALSU1''' is neighbored by [[GPNMB]] upstream and [[IGF2BP3]] downstream, however the latter gene is transcribed on the opposite strand running from the 3' to the 5' end.  There is some slight overlap of the untranslated regions of C7ORF30 and IGF2BP3 whereas the distance between C7ORF30 and GPNMB is 24,211 base pairs. 
<!-- Image with unknown copyright status removed: [[image:C7ORF30 Neighboring Genes.jpg|center|Genes surrounding C7ORF30.]]       -->

== References ==
{{Reflist}}

==External links==
* {{UCSC gene info|MALSU1}}