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'''Cleavage and polyadenylation specificity factor subunit 1''' is a [[protein]] that in humans is encoded by the ''CPSF1'' [[gene]].<ref name="pmid7651824">{{cite journal | vauthors = Jenny A, Keller W | title = Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor | journal = Nucleic Acids Res | volume = 23 | issue = 14 | pages = 2629–35 |date=Sep 1995 | pmid = 7651824 | pmc = 307085 | doi =10.1093/nar/23.14.2629  }}</ref><ref name="pmid7590244">{{cite journal | vauthors = Murthy KG, Manley JL | title = The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation | journal = Genes Dev | volume = 9 | issue = 21 | pages = 2672–83 |date=Dec 1995 | pmid = 7590244 | pmc =  | doi =10.1101/gad.9.21.2672  }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: CPSF1 cleavage and polyadenylation specific factor 1, 160kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=29894| accessdate = }}</ref>
'''Cleavage and polyadenylation specificity factor subunit 1''' is a [[protein]] that in humans is encoded by the ''CPSF1'' [[gene]].<ref name="pmid7651824">{{cite journal | vauthors = Jenny A, Keller W | title = Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor | journal = Nucleic Acids Res | volume = 23 | issue = 14 | pages = 2629–35 |date=Sep 1995 | pmid = 7651824 | pmc = 307085 | doi =10.1093/nar/23.14.2629  }}</ref><ref name="pmid7590244">{{cite journal | vauthors = Murthy KG, Manley JL | title = The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation | journal = Genes Dev | volume = 9 | issue = 21 | pages = 2672–83 |date=Dec 1995 | pmid = 7590244 | pmc =  | doi =10.1101/gad.9.21.2672  }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: CPSF1 cleavage and polyadenylation specific factor 1, 160kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=29894| accessdate = }}</ref>


In most cases eukaryotic pre-messenger(m)RNA 3 prime ends are processed in two coordinated steps.  First there is a site-specific cleavage by an endonuclease and then the addition of a poly(A) tail at the 3 prime end of the 5 prime cleavage product.  Cleavage requires four multisubunit complexes, namely cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), cleavage factors Im and IIm (CFIm and CFIIm), along with a single subunit poly(A)polymerase (PAP). CPSF1 is the largest component of the CPSF complex composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1, and located in the nucleus.  CPSF1 recognizes the AAUAAA sequence leading to [[Polyadenylation|addition of poly(A)]] to the 3-prime end of the mRNA.<ref>{{cite journal|vauthors=Yang Q, Doublié S|title=Structural biology of poly(A) site definition|journal=Wiley Interdiscip Rev RNA|year=2011|volume=2|pages=723–747|pmid=21823232|doi=10.1002/wrna.88|issue=5|pmc=3153407}}</ref>
In most cases eukaryotic [[precursor mRNA|pre-messenger(m)RNA]] 3 prime ends are processed in two coordinated steps.  First there is a site-specific cleavage by an endonuclease and then the addition of a poly(A) tail at the 3 prime end of the 5 prime cleavage product.  Cleavage requires four multisubunit complexes, namely cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), cleavage factors Im and IIm (CFIm and CFIIm), along with a single subunit poly(A)polymerase (PAP). CPSF1 is the largest component of the CPSF complex composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1, and located in the nucleus.  CPSF1 recognizes the AAUAAA sequence leading to [[Polyadenylation|addition of poly(A)]] to the 3-prime end of the mRNA.<ref>{{cite journal|vauthors=Yang Q, Doublié S|title=Structural biology of poly(A) site definition|journal=Wiley Interdiscip Rev RNA|year=2011|volume=2|pages=723–747|pmid=21823232|doi=10.1002/wrna.88|issue=5|pmc=3153407}}</ref>


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==References==
==References==
{{reflist}}
{{reflist}}
==External links==
* {{UCSC gene info|CPSF1}}


==Further reading==
==Further reading==
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{{refend}}
{{refend}}


==External links==
* {{UCSC gene info|CPSF1}}
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Latest revision as of 08:43, 10 January 2019

VALUE_ERROR (nil)
Identifiers
Aliases
External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

n/a

n/a

RefSeq (protein)

n/a

n/a

Location (UCSC)n/an/a
PubMed searchn/an/a
Wikidata
View/Edit Human

Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene.[1][2][3]

In most cases eukaryotic pre-messenger(m)RNA 3 prime ends are processed in two coordinated steps. First there is a site-specific cleavage by an endonuclease and then the addition of a poly(A) tail at the 3 prime end of the 5 prime cleavage product. Cleavage requires four multisubunit complexes, namely cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), cleavage factors Im and IIm (CFIm and CFIIm), along with a single subunit poly(A)polymerase (PAP). CPSF1 is the largest component of the CPSF complex composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1, and located in the nucleus. CPSF1 recognizes the AAUAAA sequence leading to addition of poly(A) to the 3-prime end of the mRNA.[4]


References

  1. Jenny A, Keller W (Sep 1995). "Cloning of cDNAs encoding the 160 kDa subunit of the bovine cleavage and polyadenylation specificity factor". Nucleic Acids Res. 23 (14): 2629–35. doi:10.1093/nar/23.14.2629. PMC 307085. PMID 7651824.
  2. Murthy KG, Manley JL (Dec 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes Dev. 9 (21): 2672–83. doi:10.1101/gad.9.21.2672. PMID 7590244.
  3. "Entrez Gene: CPSF1 cleavage and polyadenylation specific factor 1, 160kDa".
  4. Yang Q, Doublié S (2011). "Structural biology of poly(A) site definition". Wiley Interdiscip Rev RNA. 2 (5): 723–747. doi:10.1002/wrna.88. PMC 3153407. PMID 21823232.

Further reading

External links