ZW10

VALUE_ERROR (nil)
Identifiers
Aliases
External IDs	GeneCards: [1]
Orthologs
Species	Human	Mouse
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a
Wikidata
View/Edit Human

Centromere/kinetochore protein zw10 homolog is a protein that in humans is encoded by the ZW10 gene. This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase.

Function

Zw10
Identifiers
Symbol	Zw10
Pfam	PF06248
Pfam clan	CL0295
InterPro	IPR009361
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Zeste white 10 (ZW10) was initially identified as a mitotic checkpoint protein involved in chromosome segregation, and then implicated in targeting cytoplasmic dynein and dynactin to mitotic kinetochores, but it is also important in non-dividing cells. These include cytoplasmic dynein targeting to Golgi and other membranes, and SNARE-mediated ER-Golgi trafficking. Dominant-negative ZW10, anti-ZW10 antibody, and ZW10 RNA interference (RNAi) cause Golgi dispersal. ZW10 RNAi also disperse endosomes and lysosomes.

Drosophila kinetochore components Rough deal (Rod) and Zw10 are required for the proper functioning of the metaphase checkpoint in flies. The eukaryotic spindle assembly checkpoint (SAC) monitors microtubule attachment to kinetochores and prevents anaphase onset until all kinetochores are aligned on the metaphase plate. It is an essential surveillance mechanism that ensures high fidelity chromosome segregation during mitosis. In higher eukaryotes, cytoplasmic dynein is involved in silencing the SAC by removing the checkpoint proteins Mad2 and the Rod-Zw10-Zwilch complex (RZZ) from aligned kinetochores.

Interactions

ZW10 has been shown to interact with RINT1

Further reading

• Starr DA, Williams BC, Hays TS, Goldberg ML (1998). "ZW10 helps recruit dynactin and dynein to the kinetochore". J. Cell Biol. 142 (3): 763–74. doi:10.1083/jcb.142.3.763. PMC 2148168. PMID 9700164.
• Starr DA, Saffery R, Li Z, et al. (2000). "HZwint-1, a novel human kinetochore component that interacts with HZW10". J. Cell Sci. 113 (11): 1939–50. PMID 10806105.
• Chan GK, Jablonski SA, Starr DA, et al. (2001). "Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores". Nat. Cell Biol. 2 (12): 944–7. doi:10.1038/35046598. PMID 11146660.
• Scaërou F, Starr DA, Piano F, et al. (2001). "The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore". J. Cell Sci. 114 (Pt 17): 3103–14. PMID 11590237.
• Gevaert K, Goethals M, Martens L, et al. (2004). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nat. Biotechnol. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801.
• Hirose H, Arasaki K, Dohmae N, et al. (2005). "Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi". EMBO J. 23 (6): 1267–78. doi:10.1038/sj.emboj.7600135. PMC 381410. PMID 15029241.
• Musio A, Mariani T, Montagna C, et al. (2004). "Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes". Gene. 331: 33–40. doi:10.1016/j.gene.2004.01.028. PMID 15094189.
• Nakajima K, Hirose H, Taniguchi M, et al. (2005). "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion". EMBO J. 23 (16): 3216–26. doi:10.1038/sj.emboj.7600333. PMC 514507. PMID 15272311.
• Wang H, Hu X, Ding X, et al. (2005). "Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling". J. Biol. Chem. 279 (52): 54590–8. doi:10.1074/jbc.M407588200. PMID 15485811.
• Kops GJ, Kim Y, Weaver BA, et al. (2005). "ZW10 links mitotic checkpoint signaling to the structural kinetochore". J. Cell Biol. 169 (1): 49–60. doi:10.1083/jcb.200411118. PMC 1351127. PMID 15824131.
• Varma D, Dujardin DL, Stehman SA, Vallee RB (2006). "Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function". J. Cell Biol. 172 (5): 655–62. doi:10.1083/jcb.200510120. PMC 2063698. PMID 16505164.
• Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
• Arasaki K, Taniguchi M, Tani K, Tagaya M (2006). "RINT-1 regulates the localization and entry of ZW10 to the syntaxin 18 complex". Mol. Biol. Cell. 17 (6): 2780–8. doi:10.1091/mbc.E05-10-0973. PMC 1474792. PMID 16571679.
• Lin YT, Chen Y, Wu G, Lee WH (2006). "Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control". Oncogene. 25 (52): 6901–14. doi:10.1038/sj.onc.1209687. PMID 16732327.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
• Arasaki K, Uemura T, Tani K, Tagaya M (2007). "Correlation of Golgi localization of ZW10 and centrosomal accumulation of dynactin". Biochem. Biophys. Res. Commun. 359 (3): 811–6. doi:10.1016/j.bbrc.2007.05.188. PMID 17560939.
• Sun Y, Shestakova A, Hunt L, et al. (2007). "Rab6 regulates both ZW10/RINT-1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis". Mol. Biol. Cell. 18 (10): 4129–42. doi:10.1091/mbc.E07-01-0080. PMC 1995728. PMID 17699596.