- vanillate + O2 + NADH + H+ 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is vanillate:oxygen oxidoreductase (demethylating). Other names in common use include 4-hydroxy-3-methoxybenzoate demethylase, and vanillate demethylase. This enzyme participates in 2,4-dichlorobenzoate degradation.
- IUBMB entry for 188.8.131.52
- BRENDA references for 184.108.40.206 (Recommended.)
- PubMed references for 220.127.116.11
- PubMed Central references for 18.104.22.168
- Google Scholar references for 22.214.171.124
- Brunel F, Davison J (1988). "Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase". J. Bacteriol. 170: 4924&ndash, 30. PMID 3170489.
- Priefert H, Rabenhorst J, Steinbuchel A (1997). "Molecular characterization of genes of Pseudomonas sp. strain HR199 involved in bioconversion of vanillin to protocatechuate". J. Bacteriol. 179: 2595&ndash, 607. PMID 9098058.
Gene Ontology (GO) codes