Tris

You don't need to be Editor-In-Chief to add or edit content to WikiDoc. You can begin to add to or edit text on this WikiDoc page by clicking on the edit button at the top of this page. Next enter or edit the information that you would like to appear here. Once you are done editing, scroll down and click the Save page button at the bottom of the page.

Tris
Image:Tris.png
IUPAC name	2-Amino-2-(hydroxymethyl)propane-1,3-diol
Other names	TRIS, Tris, Tris base, Tris buffer, TrizmaTM, Trisamine, THAM, Tromethamine, Trometamol, Tromethane
Identifiers
CAS number	77-86-1
RTECS number	TY2900000
SMILES	C(C(CO)(CO)N)O
Properties
Molecular formula	C4H11NO3
Molar mass	121.135 g/mol
Appearance	White crystalline powder
Density	? g/l, solid (25°C)
Melting point	>170°C (443 K)
Boiling point	219°C (492 K)
Solubility in water	67 g/100 ml (20°C)
Acidity (pKa)	8.3
Hazards
MSDS	External MSDS
Main hazards	Irritant
NFPA 704	0 2 0
R-phrases	R36, R37, R38.
S-phrases	S26, S36.
Flash point	Non-flammable
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references

Tris is an abbreviation of the trivial name (trishydroxymethylaminomethane) for 2-amino-2-hydroxymethyl-1,3-propanediol. It is widely used as a component of buffer solutions, such as in TAE and TBE buffers used in biochemistry, with an effective pH range between 6.5 and 9.7. Tris has labile protons with a pKa of 8.30 (at 20 °C; this declines approximately 0.03 units per degree Celsius rise in temperature). Tris is often used when working with nucleic acids. Tris is an effective buffer for slightly basic solutions, which keeps DNA deprotonated and soluble in water.

Tris is commonly combined with EDTA to make TE buffer for stabilization and storage of DNA. EDTA binds to divalent cations, particularly magnesium (Mg²⁺). These ions are necessary co-factors for many enzymes; Magnesium is a co-factor for many DNA-modifying enzymes.

Tris is toxic to mammalian cells, and reacts strongly with pH electrodes. It is a primary amine, and can thus react with aldehydes.

Uses

Tris is used as an intermediate for the preparation of surface active agents, vulcanization accelerators, and pharmaceuticals, and used as a titrimetric standard.

References

This article does not cite any references or sources. ({{subst:MONTHOFYEAR}} {{subst:YEAR}}) Please help improve this article by adding citations to reliable sources. Unverifiable material may be challenged and removed.

[[Category:Articles lacking sources from {{subst:MONTHOFYEAR}} {{subst:YEAR}}]]

External links

• Using Tris Buffer in Biochemistry and Molecular Biology Experimentsde:TRIS