Peptidylglycine monooxygenase

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In enzymology, a peptidylglycine monooxygenase (EC is an enzyme that catalyzes the chemical reaction

peptidylglycine + ascorbate + O2 peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

The 3 substrates of this enzyme are peptidylglycine, ascorbate, and O2, whereas its 3 products are peptidyl(2-hydroxyglycine), dehydroascorbate, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with reduced ascorbate as one donor, and incorporation of one ato of oxygen into the other donor. The systematic name of this enzyme class is peptidylglycine,ascorbate:oxygen oxidoreductase (2-hydroxylating). Other names in common use include peptidylglycine 2-hydroxylase, peptidyl alpha-amidating enzyme, peptide-alpha-amide synthetase, synthase, peptide alpha-amide, peptide alpha-amidating enzyme, peptide alpha-amide synthase, peptidylglycine alpha-hydroxylase, peptidylglycine alpha-amidating monooxygenase, PAM-A, PAM-B, and PAM. It employs one cofactor, copper.

Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1OPM, 1PHM, 1SDW, 1YI9, 1YIP, 1YJK, 1YJL, and 3PHM.


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  • Bradbury AF, Finnie MD, Smyth DG (1982). "Mechanism of C-terminal amide formation by pituitary enzymes". Nature. 298: 686&ndash, 8. PMID 7099265.
  • Bradbury AF, Smyth DG (1987). "Enzyme-catalysed peptide amidation. Isolation of a stable intermediate formed by reaction of the amidating enzyme with an imino acid". Eur. J. Biochem. 169: 579&ndash, 84. PMID 3691506.
  • Glembotski CC (1985). "Further characterization of the peptidyl alpha-amidating enzyme in rat anterior pituitary secretory granules". Arch. Biochem. Biophys. 241: 673&ndash, 83. PMID 2994573.
  • Katopodis AG, Ping D, May SW (1990). "A novel enzyme from bovine neurointermediate pituitary catalyzes dealkylation of alpha-hydroxyglycine derivatives, thereby functioning sequentially with peptidylglycine alpha-amidating monooxygenase in peptide amidation". Biochemistry. 29: 6115&ndash, 20. PMID 2207061.
  • Murthy AS, Keutmann HT, Eipper BA (1987). "Further characterization of peptidylglycine alpha-amidating monooxygenase from bovine neurointermediate pituitary". Mol. Endocrinol. 1: 290&ndash, 9. PMID 3453894.
  • Murthy AS, Mains RE, Eipper BA (1986). "Purification and characterization of peptidylglycine alpha-amidating monooxygenase from bovine neurointermediate pituitary". J. Biol. Chem. 261: 1815&ndash, 22. PMID 3944110.

External links

The CAS registry number for this enzyme class is 90597-47-0.

Gene Ontology (GO) codes