Nicotine dehydrogenase

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In enzymology, a nicotine dehydrogenase (EC is an enzyme that catalyzes the chemical reaction

(S)-nicotine + acceptor + H2O (S)-6-hydroxynicotine + reduced acceptor

The 3 substrates of this enzyme are (S)-nicotine, acceptor, and H2O, whereas its two products are (S)-6-hydroxynicotine and reduced acceptor.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with other acceptors. The systematic name of this enzyme class is nicotine:acceptor 6-oxidoreductase (hydroxylating). Other names in common use include nicotine oxidase, D-nicotine oxidase, nicotine:(acceptor) 6-oxidoreductase (hydroxylating), and L-nicotine oxidase. It has 2 cofactors: metal, and FMN.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
  • PubMed references for
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  • Google Scholar references for
  • BEHRMAN EJ, STANIER RY (1957). "The bacterial oxidation of nicotinic acid". J. Biol. Chem. 228: 923&ndash, 45. PMID 13475371.
  • Decker K, Bleeg H (1965). "Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans". Biochim. Biophys. Acta. 105: 313&ndash, 24. PMID 5849820.
  • Hochstein LI, Dalton BP (1967). "The purification and properties of nicotine oxidase". Biochim. Biophys. Acta. 139: 56&ndash, 68. PMID 4962139.
  • HOCHSTEIN LI, RITTENBERG SC (1959). "The bacterial oxidation of nicotine. II. The isolation of the first oxidative product and its identification as (1)-6-hydroxynicotine". J. Biol. Chem. 234: 156&ndash, 60. PMID 13610912.

External links

The CAS registry number for this enzyme class is 37256-31-8.

Gene Ontology (GO) codes