Dihydrofolate synthase

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In enzymology, a dihydrofolate synthase (EC 6.3.2.12) is an enzyme that catalyzes the chemical reaction

ATP + 7,8-dihydropteroate + L-glutamate ADP + phosphate + 7,8-dihydropteroylglutamate

The 3 substrates of this enzyme are ATP, 7,8-dihydropteroate, and L-glutamate, whereas its 3 products are ADP, phosphate, and 7,8-dihydropteroylglutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is 7,8-dihydropteroate:L-glutamate ligase (ADP-forming). Other names in common use include dihydrofolate synthetase, 7,8-dihydrofolate synthetase, H2-folate synthetase, 7,8-dihydropteroate:L-glutamate ligase (ADP), dihydrofolate synthetase-folylpolyglutamate synthetase, folylpoly-(gamma-glutamate) synthetase-dihydrofolate synthase, FHFS, FHFS/FPGS, dihydropteroate:L-glutamate ligase (ADP-forming), and DHFS. This enzyme participates in folate biosynthesis.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1W78, 1W7K, and 2BMB.

References

  • IUBMB entry for 6.3.2.12
  • BRENDA references for 6.3.2.12 (Recommended.)
  • PubMed references for 6.3.2.12
  • PubMed Central references for 6.3.2.12
  • Google Scholar references for 6.3.2.12
  • GRIFFIN MJ, BROWN GM (1964). "THE BIOSYNTHESIS OF FOLIC ACID. III. ENZYMATIC FORMATION OF DIHYDROFOLIC ACID FROM DIHYDROPTEROIC ACID AND OF TETRAHYDROPTEROYLPOLYGLUTAMIC ACID COMPOUNDS FROM TETRAHYDROFOLIC ACID". J. Biol. Chem. 239: 310&ndash, 6. PMID 14114858.
  • Bognar AL, Osborne C, Shane B, Singer SC, Ferone R (1985). "Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product". J. Biol. Chem. 260: 5625&ndash, 30. PMID 2985605.
  • R, Rebeille F (2001). "Tetrahydrofolate biosynthesis in plants: molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana". Proc. Natl. Acad. Sci. U. S. A. 98: 15360&ndash, 5. PMID 11752472.
  • Cherest H, Thomas D, Surdin-Kerjan Y (2000). "Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae". J. Biol. Chem. 275: 14056&ndash, 63. PMID 10799479.
  • Cossins EA, Chen L (1997). "Folates and one-carbon metabolism in plants and fungi". Phytochemistry. 45: 437&ndash, 52. PMID 9190084.

External links

The CAS registry number for this enzyme class is 37318-62-0.

Gene Ontology (GO) codes

Template:Ligase-stub


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