The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.[3]
↑Keyse SM, Emslie EA (Oct 1992). "Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase". Nature. 359 (6396): 644–7. doi:10.1038/359644a0. PMID1406996.
↑Martell KJ, Kwak S, Hakes DJ, Dixon JE, Trent JM (Jul 1994). "Chromosomal localization of four human VH1-like protein-tyrosine phosphatases". Genomics. 22 (2): 462–4. doi:10.1006/geno.1994.1411. PMID7806236.
↑Tanoue T, Yamamoto T, Maeda R, Nishida E (Jul 2001). "A Novel MAPK phosphatase MKP-7 acts preferentially on JNK/SAPK and p38 alpha and beta MAPKs". The Journal of Biological Chemistry. 276 (28): 26629–39. doi:10.1074/jbc.M101981200. PMID11359773.
↑ 5.05.15.2Slack DN, Seternes OM, Gabrielsen M, Keyse SM (May 2001). "Distinct binding determinants for ERK2/p38alpha and JNK map kinases mediate catalytic activation and substrate selectivity of map kinase phosphatase-1". The Journal of Biological Chemistry. 276 (19): 16491–500. doi:10.1074/jbc.M010966200. PMID11278799.
↑Calvisi DF, Pinna F, Meloni F, Ladu S, Pellegrino R, Sini M, Daino L, Simile MM, De Miglio MR, Virdis P, Frau M, Tomasi ML, Seddaiu MA, Muroni MR, Feo F, Pascale RM (Jun 2008). "Dual-specificity phosphatase 1 ubiquitination in extracellular signal-regulated kinase-mediated control of growth in human hepatocellular carcinoma". Cancer Research. 68 (11): 4192–200. doi:10.1158/0008-5472.CAN-07-6157. PMID18519678.
Further reading
Martell KJ, Angelotti T, Ullrich A (Feb 1998). "The "VH1-like" dual-specificity protein tyrosine phosphatases". Molecules and Cells. 8 (1): 2–11. PMID9571625.
Keyse SM (Apr 1998). "Protein phosphatases and the regulation of MAP kinase activity". Seminars in Cell & Developmental Biology. 9 (2): 143–52. doi:10.1006/scdb.1997.0219. PMID9599409.
Abraham SM, Clark AR (Dec 2006). "Dual-specificity phosphatase 1: a critical regulator of innate immune responses". Biochemical Society Transactions. 34 (Pt 6): 1018–23. doi:10.1042/BST0341018. PMID17073741. Check date values in: |year= / |date= mismatch (help)
Raingeaud J, Gupta S, Rogers JS, Dickens M, Han J, Ulevitch RJ, Davis RJ (Mar 1995). "Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine". The Journal of Biological Chemistry. 270 (13): 7420–6. doi:10.1074/jbc.270.13.7420. PMID7535770.
Kwak SP, Hakes DJ, Martell KJ, Dixon JE (Feb 1994). "Isolation and characterization of a human dual specificity protein-tyrosine phosphatase gene". The Journal of Biological Chemistry. 269 (5): 3596–604. PMID8106404.
Emslie EA, Jones TA, Sheer D, Keyse SM (May 1994). "The CL100 gene, which encodes a dual specificity (Tyr/Thr) MAP kinase phosphatase, is highly conserved and maps to human chromosome 5q34". Human Genetics. 93 (5): 513–6. doi:10.1007/BF00202814. PMID8168826.
Sun H, Charles CH, Lau LF, Tonks NK (Nov 1993). "MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo". Cell. 75 (3): 487–93. doi:10.1016/0092-8674(93)90383-2. PMID8221888.
Alessi DR, Smythe C, Keyse SM (Jul 1993). "The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts". Oncogene. 8 (7): 2015–20. PMID8390041.
Slack DN, Seternes OM, Gabrielsen M, Keyse SM (May 2001). "Distinct binding determinants for ERK2/p38alpha and JNK map kinases mediate catalytic activation and substrate selectivity of map kinase phosphatase-1". The Journal of Biological Chemistry. 276 (19): 16491–500. doi:10.1074/jbc.M010966200. PMID11278799.
Manzano RG, Montuenga LM, Dayton M, Dent P, Kinoshita I, Vicent S, Gardner GJ, Nguyen P, Choi YH, Trepel J, Auersperg N, Birrer MJ (Jun 2002). "CL100 expression is down-regulated in advanced epithelial ovarian cancer and its re-expression decreases its malignant potential". Oncogene. 21 (28): 4435–47. doi:10.1038/sj.onc.1205542. PMID12080474.
Imasato A, Desbois-Mouthon C, Han J, Kai H, Cato AC, Akira S, Li JD (Dec 2002). "Inhibition of p38 MAPK by glucocorticoids via induction of MAPK phosphatase-1 enhances nontypeable Haemophilus influenzae-induced expression of toll-like receptor 2". The Journal of Biological Chemistry. 277 (49): 47444–50. doi:10.1074/jbc.M208140200. PMID12356755. Check date values in: |year= / |date= mismatch (help)
Denkert C, Schmitt WD, Berger S, Reles A, Pest S, Siegert A, Lichtenegger W, Dietel M, Hauptmann S (Dec 2002). "Expression of mitogen-activated protein kinase phosphatase-1 (MKP-1) in primary human ovarian carcinoma". International Journal of Cancer. 102 (5): 507–13. doi:10.1002/ijc.10746. PMID12432554.
